cDNA Clone Mint3

• Plasmid: pEBG Mint3
• Parent Plasmid: pEBG6P
• Expressed: GST-Mint3
• Genbank: NM_004886.3
• Tag: GST
• Species: Human

Sequence of Insert

CGGATCCCCACAATTTCCCGATCCCCTTCGGGGCCTCCAGCCATGGACTTGGAGGGGCCCAGGGACATTCTTGTGCCTTCGGAGGACCTCACCCCTGACAGCCAGTGGGACCCTATGCCAGGAGGCCCCGGCAGCCTCAGTCGGATGGAACTTGATGAGTCAAGCCTTCAGGAGCTGGTGCAGCAGTTTGAAGCTCTGCCAGGCGATCTGGTGGGCCCATCCCCAGGTGGAGCCCCCTGTCCCCTACACATTGCCACAGGCCATGGCCTGGCCTCCCAGGAGATTGCTGATGCCCACGGGCTCCTGTCTGCCGAAGCTGGCCGGGATGACCTGCTGGGCCTCTTGCACTGCGAGGAATGCCCGCCTTCCCAGACTGGTCCTGAAGAGCCTCTAGAGCCTGCCCCCCGACTGTTGCAGCCCCCTGAGGACCCAGATGAGGATTCTGACTCCCCAGAATGGGTGGAGGGGGCCTCTGCTGAGCAGGAGGGTAGCAGGAGCTCAAGCAGTTCCCCGGAACCCTGGCTGGAGACGGTGCCTCTAGTCACACCTGAAGAGCCACCCGCTGGTGCCCAGAGCCCCGAGACCCTGGCTTCCTACCCTGCCCCCCAGGAGGTGCCTGGTCCCTGTGACCATGAAGACCTCCTGGACGGTGTCATATTTGGGGCCAGGTACCTGGGGTCCACCCAGCTGGTGTCGGAACGGAACCCGCCCACCAGCACGCGCATGGCCCAGGCCCGGGAGGCCATGGACCGCGTCAAGGCCCCCGATGGGGAGACCCAGCCCATGACGGAGGTGGACCCGTTCGCCTCCACCAAGAGGATCAAGGTCTTGACAGCGGACTCCCAGGAGGCCATGATGGACCACGCCCTGCATACCATCTCCTACACAGCCGACATTGGCTGCGTGCTGGTGCTGATGGCGCGGCGGCGGCTGGCACGGAGGCCGGCACCCCAGGACCACGGCCGCCGCCTCTACAAGATGCTCTGCCACGTATTCTACGCAGAGGACGCCCAGCTCATCGCCCAGGCCATTGGCCAGGCCTTCGCCGCCGCCTACAGCCAGTTCCTACGGGAAAGCGGTATTGACCCCAGCCAGGTGGGCGTGCACCCGAGCCCAGGCGCCCGCCACCTCCATAATGGGGACCTGGACCACTTCTCCAACAGTGACAACTGCCGGGAGGTGCACCTCGAGAAGCGGCGAGGGGAGGGCCTGGGCGTGGCCCTGGTGGAGTCGGGCTGGGGCTCCCTGCTGCCCACAGCCGTCATCGCCAACCTGCTGCACGGGGGGCCTGCAGAGCGCTCGGGGGCCCTCAGCATCGGGGACCGCCTGACCGCCATCAACGGGACCAGCCTGGTGGGGCTGCCCCTGGCTGCGTGCCAGGCCGCTGTCCGCGAGACGAAGTCGCAGACGTCGGTGACACTCAGCATCGTCCACTGCCCTCCCGTCACCACCGCCATCATCCACCGGCCCCACGCCCGCGAGCAGCTGGGCTTCTGCGTGGAGGACGGCATCATCTGCAGCCTCCTCCGTGGTGGCATCGCCGAGCGTGGGGGCATCCGCGTCGGCCACCGCATCATTGAGATCAATGGGCAGAGTGTGGTGGCCACGCCACACGCCCGCATCATCGAGCTGCTCACCGAGGCCTATGGCGAGGTGCATATCAAGACGATGCCAGCTGCCACATATCGCCTCCTTACAGGCCAGGAGCAGCCCGTGTACCTGTGAGCGGCCGC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLVPRGSRIPTISRSPSGPPAMDLEGPRDILVPSEDLTPDSQWDPMPGGPGSLSRMELDESSLQELVQQFEALPGDLVGPSPGGAPCPLHIATGHGLASQEIADAHGLLSAEAGRDDLLGLLHCEECPPSQTGPEEPLEPAPRLLQPPEDPDEDSDSPEWVEGASAEQEGSRSSSSSPEPWLETVPLVTPEEPPAGAQSPETLASYPAPQEVPGPCDHEDLLDGVIFGARYLGSTQLVSERNPPTSTRMAQAREAMDRVKAPDGETQPMTEVDPFASTKRIKVLTADSQEAMMDHALHTISYTADIGCVLVLMARRRLARRPAPQDHGRRLYKMLCHVFYAEDAQLIAQAIGQAFAAAYSQFLRESGIDPSQVGVHPSPGARHLHNGDLDHFSNSDNCREVHLEKRRGEGLGVALVESGWGSLLPTAVIANLLHGGPAERSGALSIGDRLTAINGTSLVGLPLAACQAAVRETKSQTSVTLSIVHCPPVTTAIIHRPHAREQLGFCVEDGIICSLLRGGIAERGGIRVGHRIIEINGQSVVATPHARIIELLTEAYGEVHIKTMPAATYRLLTGQEQPVYL*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU214
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Starts at aa4 (Proline) of full length. Also has the following non silent mutation compared with the reference sequence stated L268P V270A. Mutation C376R is a natural occuring SNP. There are the following silent mutations G164 I300 A335 A432.