cDNA Clone SGK1

• Plasmid: pCMV-deltaN SGK1(S422D)
• Parent Plasmid: pCMV5
• Expressed: HA-SGK1(S422D)
• Genbank: XP_037046
• Tag: HA
• Species: Human

Sequence of Insert

GAATTCAGATCCGCCACCATGTATCCATATGATGTTCCAGATTATGCTAA
GATCTCCCAACCTCAGGAGCCTGAGCTTATGAATGCCAACCCTTCTCCTC
CACCAAGTCCTTCTCAGCAAATCAACCTTGGCCCGTCGTCCAATCCTCAT
GCTAAACCATCTGACTTTCACTTCTTGAAAGTGATCGGAAAGGGCAGTTT
TGGAAAGGTTCTTCTAGCAAGACACAAGGCAGAAGAAGTGTTCTATGCAG
TCAAAGTTTTACAGAAGAAAGCAATCCTGAAAAAGAAAGAGGAGAAGCAT
ATTATGTCGGAGCGGAATGTTCTGTTGAAGAATGTGAAGCACCCTTTCCT
GGTGGGCCTTCACTTCTCTTTCCAGACTGCTGACAAATTGTACTTTGTCC
TAGACTACATTAATGGTGGAGAGTTGTTCTACCATCTCCAGAGGGAACGC
TGCTTCCTGGAACCACGGGCTCGTTTCTATGCTGCTGAAATAGCCAGTGC
CTTGGGCTACCTGCATTCACTGAACATCGTTTATAGAGACTTAAAACCAG
AGAATATTTTGCTAGATTCACAGGGACACATTGTCCTTACTGATTTCGGA
CTCTGCAAGGAGAACATTGAACACAACAGCACAACATCCACCTTCTGTGG
CACGCCGGAGTATCTCGCACCTGAGGTGCTTCATAAGCAGCCTTATGACA
GGACTGTGGACTGGTGGTGCCTGGGAGCTGTCTTGTATGAGATGCTGTAT
GGCCTGCCGCCTTTTTATAGCCGAAACACAGCTGAAATGTACGACAACAT
TCTGAACAAGCCTCTCCAGCTGAAACCAAATATTACAAATTCCGCAAGAC
ACCTCCTGGAGGGCCTCCTGCAGAAGGACAGGACAAAGCGGCTCGGGGCC
AAGGATGACTTCATGGAGATTAAGAGTCATGTCTTCTTCTCCTTAATTAA
CTGGGATGATCTCATTAATAAGAAGATTACTCCCCCTTTTAACCCAAATG
TGAGTGGGCCCAACGACCTACGGCACTTTGACCCCGAGTTTACCGAAGAG
CCTGTCCCCAACTCCATTGGCAAGTCCCCTGACAGCGTCCTCGTCACAGC
CAGCGTCAAGGAAGCTGCCGAGGCTTTCCTAGGCTTTGACTATGCGCCTC
CCACGGACTCTTTCCTCTGAGTCGACGGTACCATCGATGCGGCCGCGTCG
ACTCTAGAGGATCC

Amino Acid Sequence

MYPYDVPDYAKISQPQEPELMNANPSPPPSPSQQINLGPSSNPHAKPSDF
HFLKVIGKGSFGKVLLARHKAEEVFYAVKVLQKKAILKKKEEKHIMSERN
VLLKNVKHPFLVGLHFSFQTADKLYFVLDYINGGELFYHLQRERCFLEPR
ARFYAAEIASALGYLHSLNIVYRDLKPENILLDSQGHIVLTDFGLCKENI
EHNSTTSTFCGTPEYLAPEVLHKQPYDRTVDWWCLGAVLYEMLYGLPPFY
SRNTAEMYDNILNKPLQLKPNITNSARHLLEGLLQKDRTKRLGAKDDFME
IKSHVFFSLINWDDLINKKITPPFNPNVSGPNDLRHFDPEFTEEPVPNSI
GKSPDSVLVTASVKEAAEAFLGFDYAPPTDSFL*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU166
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal HA for mammalian expression