cDNA Clone SGK1

• Plasmid: pCMV5 HA SGK1 S422D
• Parent Plasmid: pCMV5
• Expressed: HA SGK1 S422D
• Genbank: XP_037046
• Tag: HA
• Species: Human

Sequence of Insert

GAATTCAGATCCGCCACCATGTATCCATATGATGTTCCAGATTATGCTAC
GGTGAAAACTGAGGCTGCTAAGGGCACCCTCACTTACTCCAGGATGAGGG
GCATGGTGGCAATTCTCATCGCTTTCATGAAGCAGAGGAGGATGGGTCTG
AACGACTTTATTCAGAAGATTGCCAATAACTCCTATGCATGCAAACACCC
TGAAGTTCAGTCCATCTTGAAGATCTCCCAACCTCAGGAGCCTGAGCTTA
TGAATGCCAACCCTTCTCCTCCACCAAGTCCTTCTCAGCAAATCAACCTT
GGCCCGTCGTCCAATCCTCATGCTAAACCATCTGACTTTCACTTCTTGAA
AGTGATCGGAAAGGGCAGTTTTGGAAAGGTTCTTCTAGCAAGACACAAGG
CAGAAGAAGTGTTCTATGCAGTCAAAGTTTTACAGAAGAAAGCAATCCTG
AAAAAGAAAGAGGAGAAGCATATTATGTCGGAGCGGAATGTTCTGTTGAA
GAATGTGAAGCACCCTTTCCTGGTGGGCCTTCACTTCTCTTTCCAGACTG
CTGACAAATTGTACTTTGTCCTAGACTACATTAATGGTGGAGAGTTGTTC
TACCATCTCCAGAGGGAACGCTGCTTCCTGGAACCACGGGCTCGTTTCTA
TGCTGCTGAAATAGCCAGTGCCTTGGGCTACCTGCATTCACTGAACATCG
TTTATAGAGACTTAAAACCAGAGAATATTTTGCTAGATTCACAGGGACAC
ATTGTCCTTACTGATTTCGGACTCTGCAAGGAGAACATTGAACACAACAG
CACAACATCCACCTTCTGTGGCACGCCGGAGTATCTCGCACCTGAGGTGC
TTCATAAGCAGCCTTATGACAGGACTGTGGACTGGTGGTGCCTGGGAGCT
GTCTTGTATGAGATGCTGTATGGCCTGCCGCCTTTTTATAGCCGAAACAC
AGCTGAAATGTACGACAACATTCTGAACAAGCCTCTCCAGCTGAAACCAA
ATATTACAAATTCCGCAAGACACCTCCTGGAGGGCCTCCTGCAGAAGGAC
AGGACAAAGCGGCTCGGGGCCAAGGATGACTTCATGGAGATTAAGAGTCA
TGTCTTCTTCTCCTTAATTAACTGGGATGATCTCATTAATAAGAAGATTA
CTCCCCCTTTTAACCCAAATGTGAGTGGGCCCAACGACCTACGGCACTTT
GACCCCGAGTTTACCGAAGAGCCTGTCCCCAACTCCATTGGCAAGTCCCC
TGACAGCGTCCTCGTCACAGCCAGCGTCAAGGAAGCTGCCGAGGCTTTCC
TAGGCTTTGACTATGCGCCTCCCACGGACTCTTTCCTCTGAGTCGAC

Amino Acid Sequence

MYPYDVPDYATVKTEAAKGTLTYSRMRGMVAILIAFMKQRRMGLNDFIQK
IANNSYACKHPEVQSILKISQPQEPELMNANPSPPPSPSQQINLGPSSNP
HAKPSDFHFLKVIGKGSFGKVLLARHKAEEVFYAVKVLQKKAILKKKEEK
HIMSERNVLLKNVKHPFLVGLHFSFQTADKLYFVLDYINGGELFYHLQRE
RCFLEPRARFYAAEIASALGYLHSLNIVYRDLKPENILLDSQGHIVLTDF
GLCKENIEHNSTTSTFCGTPEYLAPEVLHKQPYDRTVDWWCLGAVLYEML
YGLPPFYSRNTAEMYDNILNKPLQLKPNITNSARHLLEGLLQKDRTKRLG
AKDDFMEIKSHVFFSLINWDDLINKKITPPFNPNVSGPNELRHFDPEFTE
EPVPNSIGKSPDSVLVTASVKEAAEAFLGFDYAPPTDSFL*

• Vector Type: Mammalian
• Antibiotic: Amp
• Full Snapgene Map: pCMV5 HA SGK1 S422D56.21 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU158
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal HA for mammalian expression
• Low Temp: No