cDNA Clone SGK1

• Plasmid: pCMV SGK1
• Parent Plasmid: pCMV5
• Expressed: HA-SGK1
• Genbank: XP_037046
• Tag: HA
• Species: Human

Sequence of Insert

GAATTCAGATCCGCCACCATGTATCCATATGATGTTCCAGATTATGCTAC
GGTGAAAACTGAGGCTGCTAAGGGCACCCTCACTTACTCCAGGATGAGGG
GCATGGTGGCAATTCTCATCGCTTTCATGAAGCAGAGGAGGATGGGTCTG
AACGACTTTATTCAGAAGATTGCCAATAACTCCTATGCATGCAAACACCC
TGAAGTTCAGTCCATCTTGAAGATCTCCCAACCTCAGGAGCCTGAGCTTA
TGAATGCCAACCCTTCTCCTCCACCAAGTCCTTCTCAGCAAATCAACCTT
GGCCCGTCGTCCAATCCTCATGCTAAACCATCTGACTTTCACTTCTTGAA
AGTGATCGGAAAGGGCAGTTTTGGAAAGGTTCTTCTAGCAAGACACAAGG
CAGAAGAAGTGTTCTATGCAGTCAAAGTTTTACAGAAGAAAGCAATCCTG
AAAAAGAAAGAGGAGAAGCATATTATGTCGGAGCGGAATGTTCTGTTGAA
GAATGTGAAGCACCCTTTCCTGGTGGGCCTTCACTTCTCTTTCCAGACTG
CTGACAAATTGTACTTTGTCCTAGACTACATTAATGGTGGAGAGTTGTTC
TACCATCTCCAGAGGGAACGCTGCTTCCTGGAACCACGGGCTCGTTTCTA
TGCTGCTGAAATAGCCAGTGCCTTGGGCTACCTGCATTCACTGAACATCG
TTTATAGAGACTTAAAACCAGAGAATATTTTGCTAGATTCACAGGGACAC
ATTGTCCTTACTGATTTCGGACTCTGCAAGGAGAACATTGAACACAACAG
CACAACATCCACCTTCTGTGGCACGCCGGAGTATCTCGCACCTGAGGTGC
TTCATAAGCAGCCTTATGACAGGACTGTGGACTGGTGGTGCCTGGGAGCT
GTCTTGTATGAGATGCTGTATGGCCTGCCGCCTTTTTATAGCCGAAACAC
AGCTGAAATGTACGACAACATTCTGAACAAGCCTCTCCAGCTGAAACCAA
ATATTACAAATTCCGCAAGACACCTCCTGGAGGGCCTCCTGCAGAAGGAC
AGGACAAAGCGGCTCGGGGCCAAGGATGACTTCATGGAGATTAAGAGTCA
TGTCTTCTTCTCCTTAATTAACTGGGATGATCTCATTAATAAGAAGATTA
CTCCCCCTTTTAACCCAAATGTGAGTGGGCCCAACGAGCTACGGCACTTT
GACCCCGAGTTTACCGAAGAGCCTGTCCCCAACTCCATTGGCAAGTCCCC
TGACAGCGTCCTCGTCACAGCCAGCGTCAAGGAAGCTGCCGAGGCTTTCC
TAGGCTTTTCCTATGCGCCTCCCACGGACTCTTTCCTCTGAGTCGACTCT
AGAGGATCC

Amino Acid Sequence

MYPYDVPDYATVKTEAAKGTLTYSRMRGMVAILIAFMKQRRMGLNDFIQK
IANNSYACKHPEVQSILKISQPQEPELMNANPSPPPSPSQQINLGPSSNP
HAKPSDFHFLKVIGKGSFGKVLLARHKAEEVFYAVKVLQKKAILKKKEEK
HIMSERNVLLKNVKHPFLVGLHFSFQTADKLYFVLDYINGGELFYHLQRE
RCFLEPRARFYAAEIASALGYLHSLNIVYRDLKPENILLDSQGHIVLTDF
GLCKENIEHNSTTSTFCGTPEYLAPEVLHKQPYDRTVDWWCLGAVLYEML
YGLPPFYSRNTAEMYDNILNKPLQLKPNITNSARHLLEGLLQKDRTKRLG
AKDDFMEIKSHVFFSLINWDDLINKKITPPFNPNVSGPNELRHFDPEFTE
EPVPNSIGKSPDSVLVTASVKEAAEAFLGFSYAPPTDSFL*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU153
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal HA for mammalian expression