cDNA Clone SGK1

• Plasmid: pEBG delta AA1-59 SGK1
• Parent Plasmid: pEBG2T
• Expressed: GST-delta 1-59 SGK1
• Genbank: O0014
• Tag: GST
• Species: Human

Sequence of Insert

AGATCTGATCTCCCAACCTCAGGAGCCTGAGCTTATGAATGCCAACCCTT
CTCCTCCACCAAGTCCTTCTCAGCAAATCAACCTTGGCCCGTCGTCCAAT
CCTCATGCTAAACCATCTGACTTTCACTTCTTGAAAGTGATCGGAAAGGG
CAGTTTTGGAAAGGTTCTTCTAGCAAGACACAAGGCAGAAGAAGTGTTCT
ATGCAGTCAAAGTTTTACAGAAGAAAGCAATCCTGAAAAAGAAAGAGGAG
AAGCATATTATGTCGGAGCGGAATGTTCTGTTGAAGAATGTGAAGCACCC
TTTCCTGGTGGGCCTTCACTTCTCTTTCCAGACTGCTGACAAATTGTACT
TTGTCCTAGACTACATTAATGGTGGAGAGTTGTTCTACCATCTCCAGAGG
GAACGCTGCTTCCTGGAACCACGGGCTCGTTTCTATGCTGCTGAAATAGC
CAGTGCCTTGGGCTACCTGCATTCACTGAACATCGTTTATAGAGACTTAA
AACCAGAGAATATTTTGCTAGATTCACAGGGACACATTGTCCTTACTGAC
TTCGGACTCTGCAAGGAGAACATTGAACACAACAGCACAACATCCACCTT
CTGTGGCACGCCGGAGTATCTCGCACCTGAGGTGCTTCATAAGCAGCCTT
ATGACAGGACTGTGGACTGGTGGTGCCTGGGAGCTGTCTTGTATGAGATG
CTGTATGGCCTGCCGCCTTTTTATAGCCGAAACACAGCTGAAATGTACGA
CAACATTCTGAACAAGCCTCTCCAGCTGAAACCAAATATTACAAATTCCG
CAAGACACCTCCTGGAGGGCCTCCTGCAGAAGGACAGGACAAAGCGGCTC
GGGGCCAAGGATGACTTCATGGAGATTAAGAGTCATGTCTTCTTCTCCTT
AATTAACTGGGATGATCTCATTAATAAGAAGATTACTCCCCCTTTTAACC
CAAATGTGAGTGGGCCCAACGACCTACGGCACTTTGACCCCGAGTTTACC
GAAGAGCCTGTCCCCAACTCCATTGGCAAGTCCCCTGACAGCGTCCTCGT
CACAGCCAGCGTCAAGGAAGCTGCCGAGGCTTTCCTAGGCTTTTCCTATG
CGCCTCCCACGGACTCTTTCCTCTGAGTCGACGGTACCATCGATGCGGCC
GC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSISQPQEPELMNANPSPPPSPSQQI
NLGPSSNPHAKPSDFHFLKVIGKGSFGKVLLARHKAEEVFYAVKVLQKKA
ILKKKEEKHIMSERNVLLKNVKHPFLVGLHFSFQTADKLYFVLDYINGGE
LFYHLQRERCFLEPRARFYAAEIASALGYLHSLNIVYRDLKPENILLDSQ
GHIVLTDFGLCKENIEHNSTTSTFCGTPEYLAPEVLHKQPYDRTVDWWCL
GAVLYEMLYGLPPFYSRNTAEMYDNILNKPLQLKPNITNSARHLLEGLLQ
KDRTKRLGAKDDFMEIKSHVFFSLINWDDLINKKITPPFNPNVSGPNDLR
HFDPEFTEEPVPNSIGKSPDSVLVTASVKEAAEAFLGFSYAPPTDSFL*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU142
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for mammalian expression; Calc Mol Weight 68530 Da