cDNA Clone SGK1

• Plasmid: pEBG SGK1 (K127A)
• Parent Plasmid: pEBG2T
• Expressed: GST-SGK1(K127A)
• Genbank: XP_037046
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCGCCACCATGTATCCATATGATGTTCCAGATTATGCTACGGTGAA
AACTGAGGCTGCTAAGGGCACCCTCACTTACTCCAGGATGAGGGGCATGG
TGGCAATTCTCATCGCTTTCATGAAGCAGAGGAGGATGGGTCTGAACGAC
TTTATTCAGAAGATTGCCAATAACTCCTATGCATGCAAACACCCTGAAGT
TCAGTCCATCTTGAAGATCTCCCAACCTCAGGAGCCTGAGCTTATGAATG
CCAACCCTTCTCCTCCACCAAGTCCTTCTCAGCAAATCAACCTTGGCCCG
TCGTCCAATCCTCATGCTAAACCATCTGACTTTCACTTCTTGAAAGTGAT
CGGAAAGGGCAGTTTTGGAAAGGTTCTTCTAGCAAGACACAAGGCAGAAG
AAGTGTTCTATGCAGTCGCAGTTTTACAGAAGAAAGCAATCCTGAAAAAG
AAAGAGGAGAAGCATATTATGTCGGAGCGGAATGTTCTGTTGAAGAATGT
GAAGCACCCTTTCCTGGTGGGCCTTCACTTCTCTTTCCAGACTGCTGACA
AATTGTACTTTGTCCTAGACTACATTAATGGTGGAGAGTTGTTCTACCAT
CTCCAGAGGGAACGCTGCTTCCTGGAACCACGGGCTCGTTTCTATGCTGC
TGAAATAGCCAGTGCCTTGGGCTACCTGCATTCACTGAACATCGTTTATA
GAGACTTAAAACCAGAGAATATTTTGCTAGATTCACAGGGACACATTGTC
CTTACTGATTTCGGACTCTGCAAGGAGAACATTGAACACAACAGCACAAC
ATCCACCTTCTGTGGCACGCCGGAGTATCTCGCACCTGAGGTGCTTCATA
AGCAGCCTTATGACAGGACTGTGGACTGGTGGTGCCTGGGAGCTGTCTTG
TATGAGATGCTGTATGGCCTGCCGCCTTTTTATAGCCGAAACACAGCTGA
AATGTACGACAACATTCTGAACAAGCCTCTCCAGCTGAAACCAAATATTA
CAAATTCCGCAAGACACCTCCTGGAGGGCCTCCTGCAGAAGGACAGGACA
AAGCGGCTCGGGGCCAAGGATGACTTCATGGAGATTAAGAGTCATGTCTT
CTTCTCCTTAATTAACTGGGATGATCTCATTAATAAGAAGATTACTCCCC
CTTTTAACCCAAATGTGAGTGGGCCCAACGACCTACGGCACTTTGACCCC
GAGTTTACCGAAGAGCCTGTCCCCAACTCCATTGGCAAGTCCCCTGACAG
CGTCCTCGTCACAGCCAGCGTCAAGGAAGCTGCCGAGGCTTTCCTAGGCT
TTTCCTATGCGCCTCCCACGGACTCTTTCCTCTGAGTCGACTCTAGGGTA
CC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSATMYPYDVPDYATVKTEAAKGTLT
YSRMRGMVAILIAFMKQRRMGLNDFIQKIANNSYACKHPEVQSILKISQP
QEPELMNANPSPPPSPSQQINLGPSSNPHAKPSDFHFLKVIGKGSFGKVL
LARHKAEEVFYAVAVLQKKAILKKKEEKHIMSERNVLLKNVKHPFLVGLH
FSFQTADKLYFVLDYINGGELFYHLQRERCFLEPRARFYAAEIASALGYL
HSLNIVYRDLKPENILLDSQGHIVLTDFGLCKENIEHNSTTSTFCGTPEY
LAPEVLHKQPYDRTVDWWCLGAVLYEMLYGLPPFYSRNTAEMYDNILNKP
LQLKPNITNSARHLLEGLLQKDRTKRLGAKDDFMEIKSHVFFSLINWDDL
INKKITPPFNPNVSGPNDLRHFDPEFTEEPVPNSIGKSPDSVLVTASVKE
AAEAFLGFSYAPPTDSFL*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU125
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST/HA for mammalian expression. Kinase dead mutation.