cDNA Clone USP48

• Plasmid: pCMV5-HA-1 USP48 v.3
• Parent Plasmid: pCMV5 HA1
• Expressed: HA-USP48 v.3
• Genbank: Matches IMAGE:9051848
• Tag: HA
• Species: Human

Sequence of Insert

GGATCCATGGCCCCGCGGCTGCAGCTGGAGAAGGCGGCCTGGCGCTGGGC
GGAGACGGTGCGGCCCGAGGAGGTGTCGCAGGAGCACATCGAGACCGCTT
ACCGCATCTGGCTGGAGCCCTGCATTCGCGGCGTGTGCAGACGAAACTGC
AAAGGAAATCCGAATTGCTTGGTTGGTATTGGTGAGCATATTTGGTTAGG
AGAAATAGATGAAAATAGTTTTCATAACATCGATGATCCCAACTGTGAGA
GGAGAAAAAAGAACTCATTTGTGGGCCTGACTAACCTTGGAGCCACTTGT
TATGTCAACACATTTCTTCAAGTGTGGTTTCTCAACTTGGAGCTTCGGCA
GGCACTCTACTTATGTCCAAGCACTTGTAGTGACTACATGCTGGGAGACG
GCATCCAAGAAGAAAAAGATTATGAGCCTCAAACAATTTGTGAGCATCTC
CAGTACTTGTTTGCCTTGTTGCAAAACAGTAATAGGCGATACATTGATCC
ATCAGGATTTGTTAAAGCCTTGGGCCTGGACACTGGACAACAGCAGGATG
CTCAAGAATTTTCAAAGCTCTTTATGTCTCTATTGGAAGATACTTTGTCT
AAACAAAAGAATCCAGATGTGCGCAATATTGTTCAACAGCAGTTCTGTGG
AGAATATGCCTATGTAACTGTTTGCAACCAGTGTGGCAGAGAGTCTAAGC
TTTTGTCAAAATTTTATGAGCTGGAGTTAAATATCCAAGGCCACAAACAG
TTAACAGATTGTATCTCGGAATTTTTGAAGGAAGAAAAATTAGAAGGAGA
CAATCGCTATTTTTGCGAGAACTGTCAAAGCAAACAGAATGCAACAAGAA
AGATTCGACTTCTTAGCCTTCCTTGCACTCTGAACTTGCAGCTAATGCGT
TTTGTCTTTGACAGGCAAACTGGACATAAGAAAAAGCTGAATACCTACAT
TGGCTTCTCAGAAATTTTGGATATGGAGCCTTATGTGGAACATAAAGGTG
GGTCCTACGTGTATGAACTCAGCGCAGTCCTCATACACAGAGGAGTGAGT
GCTTATTCTGGCCACTACATCGCCCACGTGAAAGATCCACAGTCTGGTGA
ATGGTATAAGTTTAATGATGAAGACATAGAAAAGATGGAGGGGAAGAAAT
TACAACTAGGGATTGAGGAAGATCTAGCAGAACCTTCTAAGTCTCAGACA
CGTAAACCCAAGTGTGGCAAAGGAACTCATTGCTCTCGAAATGCATATAT
GTTGGTTTATAGACTGCAAACTCAAGAAAAGCCCAACACTACTGTTCAAG
TTCCAGCCTTTCTTCAAGAGCTGGTAGATCGGGATAATTCCAAATTTGAG
GAGTGGTGTATTGAAATGGCTGAGATGCGTAAGCAAAGTGTGGATAAAGG
AAAAGCAAAACACGAAGAGGTTAAGGAGCTGTACCAAAGGTTACCTGCTG
GAGCTGAGCCCTATGAGTTTGTCTCTCTGGAATGGCTGCAAAAGTGGTTG
GATGAATCAACACCTACCAAACCTATTGATAATCACGCTTGCCTGTGTTC
CCATGACAAGCTTCACCCGGATAAAATATCAATTATGAAGAGGATATCTG
AATATGCAGCTGACATTTTCTATAGTAGATATGGAGGAGGTCCAAGACTA
ACTGTGAAAGCCCTGTGTAAGGAATGTGTAGTAGAACGTTGTCGCATATT
GCGTCTGAAGAACCAACTAAATGAAGATTATAAAACTGTTAATAATCTGC
TGAAAGCAGCAGTAAAGGGCAGCGATGGATTTTGGGTGGGGAAGTCCTCC
TTGCGGAGTTGGCGCCAGCTAGCTCTTGAACAGCTGGATGAGCAAGATGG
TGATGCAGAACAAAGCAACGGAAAGATGAACGGTAGCACCTTAAATAAAG
ATGAATCAAAGGAAGAAAGAAAAGAAGAGGAGGAATTAAATTTTAATGAA
GATATTCTGTGTCCACATGGTGAGTTATGCATATCTGAAAATGAAAGAAG
GCTTGTTTCTAAAGAGGCTTGGAGCAAACTGCAGCAGTACTTTCCAAAGG
CTCCTGAGTTTCCAAGTTACAAAGAGTGCTGTTCACAGTGCAAGATTTTA
GAAAGAGAAGGGGAAGAAAATGAAGCCTTACATAAGATGATTGCAAACGA
GCAAAAGACTTCTCTCCCAAATTTGTTCCAGGATAAAAACAGACCGTGTC
TCAGTAACTGGCCAGAGGATACGGATGTCCTCTACATCGTGTCTCAGTTC
TTTGTAGAAGAGTGGCGGAAATTTGTTAGAAAGCCTACAAGATGCAGCCC
TGTGTCATCAGTTGGGAACAGTGCTCTTTTGTGTCCCCACGGGGGCCTCA
TGTTTACATTTGCTTCCATGACCAAAGAAGATTCTAAACTTATAGCTCTC
ATATGGCCCAGTGAGTGGCAAATGATACAAAAGCTCTTTGTTGTGGATCA
TGTAATTAAAATCACGAGAATTGAAGTGGGAGATGTAAACCCTTCAGAAA
CACAGTATATTTCTGAGCCCAAACTCTGTCCAGAATGCAGAGAAGGCTTA
TTGTGTCAGCAGCAGAGGGACCTGCGTGAATACACTCAAGCCACCATCTA
TGTCCATAAAGTTGTGGATAATAAAAAGGTGATGAAGGATTCGGCTCCGG
AACTGAATGTGAGTAGTTCTGAAACAGAGGAGGACAAGGAAGAAGCTAAA
CCAGATGGAGAAAAAGATCCAGATTTTAATCAAATCATGCATGCATTTTC
AGTTGCTCCTTTTGACCAGAATTTGTCAATTGATGGAAAGATTTTAAGTG
ATGACTGTGCCACCCTAGGCACCCTTGGCGTCATTCCTGAATCTGTCATT
TTATTGAAGGCTGATGAACCAATTGCAGATTATGCTGCAATGGATGATGT
CATGCAAGTTTGTATGCCAGAAGAAGGGTTTAAAGGTACTGGTCTTCTTG
GACATTAAGCGGCCGC

Amino Acid Sequence

MYPYDVPDYAGSMAPRLQLEKAAWRWAETVRPEEVSQEHIETAYRIWLEPCIRGVCRRNCKGNPNCLVGIGEHIWLGEIDENSFHNIDDPNCERRKKNSFVGLTNLGATCYVNTFLQVWFLNLELRQALYLCPSTCSDYMLGDGIQEEKDYEPQTICEHLQYLFALLQNSNRRYIDPSGFVKALGLDTGQQQDAQEFSKLFMSLLEDTLSKQKNPDVRNIVQQQFCGEYAYVTVCNQCGRESKLLSKFYELELNIQGHKQLTDCISEFLKEEKLEGDNRYFCENCQSKQNATRKIRLLSLPCTLNLQLMRFVFDRQTGHKKKLNTYIGFSEILDMEPYVEHKGGSYVYELSAVLIHRGVSAYSGHYIAHVKDPQSGEWYKFNDEDIEKMEGKKLQLGIEEDLAEPSKSQTRKPKCGKGTHCSRNAYMLVYRLQTQEKPNTTVQVPAFLQELVDRDNSKFEEWCIEMAEMRKQSVDKGKAKHEEVKELYQRLPAGAEPYEFVSLEWLQKWLDESTPTKPIDNHACLCSHDKLHPDKISIMKRISEYAADIFYSRYGGGPRLTVKALCKECVVERCRILRLKNQLNEDYKTVNNLLKAAVKGSDGFWVGKSSLRSWRQLALEQLDEQDGDAEQSNGKMNGSTLNKDESKEERKEEEELNFNEDILCPHGELCISENERRLVSKEAWSKLQQYFPKAPEFPSYKECCSQCKILEREGEENEALHKMIANEQKTSLPNLFQDKNRPCLSNWPEDTDVLYIVSQFFVEEWRKFVRKPTRCSPVSSVGNSALLCPHGGLMFTFASMTKEDSKLIALIWPSEWQMIQKLFVVDHVIKITRIEVGDVNPSETQYISEPKLCPECREGLLCQQQRDLREYTQATIYVHKVVDNKKVMKDSAPELNVSSSETEEDKEEAKPDGEKDPDFNQIMHAFSVAPFDQNLSIDGKILSDDCATLGTLGVIPESVILLKADEPIADYAAMDDVMQVCMPEEGFKGTGLLGH*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU37160
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N-ter HA mammalian expression of protein of interest; ; Estimated MW of expressed protein : 114319.06 Daltons