cDNA Clone FANCD2

• Synonyms: FA-D2, FA4, FACD, FAD, FAD2, FANCD
• Plasmid: pET43 DU FANCD2
• Parent Plasmid: pET43
• Expressed: NUS-6xHis-S.TAG-Prec-FANCD2
• Genbank: NM_001018115.1
• Tag: 6His
• Species: Human

Sequence of Insert

GCGGCCGCTATGGTTTCCAAAAGAAGACTGTCAAAATCTGAGGATAAAGAGAGCCTGACAGAAGATGCCTCCAAAACCAGGAAGCAACCACTTTCCAAAAAGACAAAGAAATCTCATATTGCTAATGAAGTTGAAGAAAATGACAGCATCTTTGTAAAGCTTCTTAAGATATCAGGAATTATTCTTAAAACGGGAGAGAGTCAGAATCAACTAGCTGTGGATCAAATAGCTTTCCAAAAGAAGCTCTTTCAGACCCTGAGGAGACACCCTTCCTATCCCAAAATAATAGAAGAATTTGTTAGTGGCCTGGAGTCTTACATTGAGGATGAAGACAGTTTCAGGAACTGCCTTTTGTCTTGTGAGCGTCTGCAGGATGAGGAAGCCAGTATGGGTGCATCTTATTCTAAGAGTCTCATCAAACTGCTTCTGGGGATTGACATACTGCAGCCTGCCATTATCAAAACCTTATTTGAGAAGTTGCCAGAATATTTTTTTGAAAACAAGAACAGTGATGAAATCAACATACCTCGACTCATTGTCAGTCAACTAAAATGGCTTGACAGAGTTGTGGATGGCAAGGACCTCACCACCAAGATCATGCAGCTGATCAGTATTGCTCCAGAGAACCTGCAGCATGACATCATCACCAGCCTACCTGAGATCCTAGGGGATTCCCAGCACGCTGATGTGGGGAAAGAACTCAGTGACCTACTGATAGAGAATACTTCACTCACTGTCCCAATCCTGGATGTCCTTTCAAGCCTCCGACTTGACCCAAACTTCCTATTGAAGGTTCGCCAGTTGGTGATGGATAAGTTGTCGTCTATTAGATTGGAGGATTTACCTGTGATAATAAAGTTCATTCTTCATTCCGTAACAGCCATGGATACACTTGAGGTAATTTCTGAGCTTCGGGAGAAGTTGGATCTGCAGCATTGTGTTTTGCCATCACGGTTACAGGCTTCCCAAGTAAAGTTGAAAAGTAAAGGACGAGCAAGTTCCTCAGGAAATCAAGAAAGCAGCGGTCAGAGCTGTATTATTCTCCTCTTTGATGTAATAAAGTCAGCTATTAGATATGAGAAAACCATTTCAGAAGCCTGGATTAAGGCAATTGAAAACACTGCCTCAGTATCTGAACACAAGGTGTTTGACCTGGTGATGCTTTTCATCATCTATAGCACCAATACTCAGACAAAGAAGTACATTGACAGGGTGCTAAGAAATAAGATTCGATCAGGCTGCATTCAAGAACAGCTGCTCCAGAGTACATTCTCTGTTCATTACTTAGTTCTTAAGGATATGTGTTCATCCATTCTGTCGCTGGCTCAGAGTTTGCTTCACTCTCTAGACCAGAGTATAATTTCATTTGGCAGTCTCCTATACAAATATGCATTTAAGTTTTTTGACACGTACTGCCAGCAGGAAGTGGTTGGTGCCTTAGTGACCCATATCTGCAGTGGGAATGAAGCTGAAGTTGATACTGCCTTAGATGTCCTTCTAGAGTTGGTAGTGTTAAACCCATCTGCTATGATGATGAATGCTGTCTTTGTAAAGGGCATTTTAGATTATCTGGATAACATATCCCCTCAGCAAATACGAAAACTCTTCTATGTTCTCAGCACACTGGCATTTAGCAAACAGAATGAAGCCAGCAGCCACATCCAGGATGACATGCACTTGGTGATAAGAAAGCAGCTCTCTAGCACCGTATTCAAGTACAAGCTCATTGGGATTATTGGTGCTGTGACCATGGCTGGCATCATGGCGGCAGACAGAAGTGAATCACCTAGTTTGACCCAAGAGAGAGCCAACCTGAGCGATGAGCAGTGCACACAGGTGACCTCCTTGTTGCAGTTGGTTCATTCCTGCAGTGAGCAGTCTCCTCAGGCCTCTGCACTTTACTATGATGAATTTGCCAACCTGATCCAACATGAAAAGCTGGATCCAAAAGCCCTGGAATGGGTTGGGCATACCATCTGTAATGATTTCCAGGATGCCTTCGTAGTGGACTCCTGTGTTGTTCCGGAAGGTGACTTTCCATTTCCTGTGAAAGCACTGTACGGACTGGAAGAATACGACACTCAGGATGGGATTGCCATAAACCTCCTGCCGCTGCTGTTTTCTCAGGACTTTGCAAAAGATGGGGGTCCGGTGACCTCACAGGAATCAGGCCAAAAATTGGTGTCTCCGCTGTGCCTGGCTCCGTATTTCCGGTTACTGAGACTTTGTGTGGAGAGACAGCATAACGGAAACTTGGAGGAGATTGATGGTCTACTAGATTGTCCTATATTCCTAACTGACCTGGAGCCTGGAGAGAAGTTGGAGTCCATGTCTGCTAAAGAGCGTTCATTCATGTGTTCTCTCATATTTCTTACTCTCAACTGGTTCCGAGAGATTGTAAATGCCTTCTGCCAGGAAACATCACCTGAGATGAAGGGGAAGGTGCTCACTCGGTTAAAGCACATTGTAGAATTGCAAATAATCCTGGAAAAGTACTTGGCAGTCACCCCAGACTATGTCCCTCCTCTTGGAAACTTTGATGTGGAAACTTTAGATATAACACCTCATACTGTTACTGCTATTTCAGCAAAAATCAGAAAGAAAGGAAAAATAGAAAGGAAACAAAAAACAGATGGCAGCAAGACATCCTCCTCTGACACACTTTCAGAAGAGAAAAATTCAGAATGTGACCCTACGCCATCTCATAGAGGCCAGCTAAACAAGGAGTTCACAGGGAAGGAAGAAAAGACATCATTGTTACTACATAATTCCCATGCTTTTTTCCGAGAGCTGGACATTGAGGTCTTCTCTATTCTACATTGTGGACTTGTGACGAAGTTCATCTTAGATACTGAAATGCACACTGAAGCTACAGAAGTTGTGCAACTTGGGCCCCCTGAGCTGCTTTTCTTGCTGGAAGATCTCTCCCAGAAGCTGGAGAGTATGCTGACACCTCCTATTGCCAGGAGAGTCCCCTTTCTCAAGAACAAAGGAAGCCGGAATATTGGATTCTCACATCTCCAACAGAGATCTGCCCAAGAAATTGTTCATTGTGTTTTTCAACTGCTGACCCCAATGTGTAACCACCTGGAGAACATTCACAACTATTTTCAGTGTTTAGCTGCTGAGAATCACGGTGTAGTTGATGGACCAGGAGTGAAAGTTCAGGAGTACCACATAATGTCTTCCTGCTATCAGAGGCTGCTGCAGATTTTTCATGGGCTTTTTGCTTGGAGTGGATTTTCTCAACCTGAAAATCAGAATTTACTGTATTCAGCCCTCCATGTCCTTAGTAGCCGACTGAAACAGGGAGAACACAGCCAGCCTTTGGAGGAACTACTCAGCCAGAGCGTCCATTACTTGCAGAATTTCCATCAAAGCATTCCCAGTTTCCAGTGTGCTCTTTATCTCATCAGACTTTTGATGGTTATTTTGGAGAAATCAACAGCTTCTGCTCAGAACAAAGAAAAAATTGCTTCCCTTGCCAGACAATTCCTCTGTCGGGTGTGGCCAAGTGGGGATAAAGAGAAGAGCAACATCTCTAATGACCAGCTCCATGCTCTGCTCTGTATCTACCTGGAGCACACAGAGAGCATTCTGAAGGCCATAGAGGAGATTGCTGGTGTTGGTGTCCCAGAACTGATCAACTCTCCTAAAGATGCATCTTCCTCCACATTCCCTACACTGACCAGGCATACTTTTGTTGTTTTCTTCCGTGTGATGATGGCTGAACTAGAGAAGACGGTGAAAAAAATTGAGCCTGGCACAGCAGCAGACTCGCAGCAGATTCATGAAGAGAAACTCCTCTACTGGAACATGGCTGTTCGAGACTTCAGTATCCTCATCAACTTGATAAAGGTATTTGATAGTCATCCTGTTCTGCATGTATGTTTGAAGTATGGGCGTCTCTTTGTGGAAGCATTTCTGAAGCAATGTATGCCGCTCCTAGACTTCAGTTTTAGAAAACACCGGGAAGATGTTCTGAGCTTACTGGAAACCTTCCAGTTGGACACAAGGCTGCTTCATCACCTGTGTGGGCATTCCAAGATTCACCAGGACACGAGACTCACCCAACATGTGCCTCTGCTCAAAAAGACCCTGGAACTTTTAGTTTGCAGAGTCAAAGCTATGCTCACTCTCAACAATTGTAGAGAGGCTTTCTGGCTGGGCAATCTAAAAAACCGGGACTTGCAGGGTGAAGAGATTAAGTCCCAAAATTCCCAGGAGAGCACAGCAGATGAGAGTGAGGATGACATGTCATCCCAGGCCTCCAAGAGCAAAGCCACTGAGGATGGTGAAGAAGACGAAGTAAGTGCTGGAGAAAAGGAGCAAGATAGTGATGAGAGTTATGATGACTCTGATTAGGCGGCCGC

Amino Acid Sequence

MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEIDVRVQID
RKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESV
TFDRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNI
SLDLGNNAEAVILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRS
KPEMLIELFRIEVPEIGEEVIEIKAAARDPGSRAKIAVKTNDKRIDPVGA
CVGMRGARVQAVSTELGGERIDIVLWDDNPAQFVINAMAPADVASIVVDE
DKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGWELNVMTVDDLQAKHQA
EAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKELLEIEGLDE
PTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEATSGSG
HHHHHHSAGKETAAAKFERQHMDSPPPTGLVPRGSAGSGTIDDDDKSPGA
RLEVLFQGPGSSEFEVDSLESAAAMVSKRRLSKSEDKESLTEDASKTRKQ
PLSKKTKKSHIANEVEENDSIFVKLLKISGIILKTGESQNQLAVDQIAFQ
KKLFQTLRRHPSYPKIIEEFVSGLESYIEDEDSFRNCLLSCERLQDEEAS
MGASYSKSLIKLLLGIDILQPAIIKTLFEKLPEYFFENKNSDEINIPRLI
VSQLKWLDRVVDGKDLTTKIMQLISIAPENLQHDIITSLPEILGDSQHAD
VGKELSDLLIENTSLTVPILDVLSSLRLDPNFLLKVRQLVMDKLSSIRLE
DLPVIIKFILHSVTAMDTLEVISELREKLDLQHCVLPSRLQASQVKLKSK
GRASSSGNQESSGQSCIILLFDVIKSAIRYEKTISEAWIKAIENTASVSE
HKVFDLVMLFIIYSTNTQTKKYIDRVLRNKIRSGCIQEQLLQSTFSVHYL
VLKDMCSSILSLAQSLLHSLDQSIISFGSLLYKYAFKFFDTYCQQEVVGA
LVTHICSGNEAEVDTALDVLLELVVLNPSAMMMNAVFVKGILDYLDNISP
QQIRKLFYVLSTLAFSKQNEASSHIQDDMHLVIRKQLSSTVFKYKLIGII
GAVTMAGIMAADRSESPSLTQERANLSDEQCTQVTSLLQLVHSCSEQSPQ
ASALYYDEFANLIQHEKLDPKALEWVGHTICNDFQDAFVVDSCVVPEGDF
PFPVKALYGLEEYDTQDGIAINLLPLLFSQDFAKDGGPVTSQESGQKLVS
PLCLAPYFRLLRLCVERQHNGNLEEIDGLLDCPIFLTDLEPGEKLESMSA
KERSFMCSLIFLTLNWFREIVNAFCQETSPEMKGKVLTRLKHIVELQIIL
EKYLAVTPDYVPPLGNFDVETLDITPHTVTAISAKIRKKGKIERKQKTDG
SKTSSSDTLSEEKNSECDPTPSHRGQLNKEFTGKEEKTSLLLHNSHAFFR
ELDIEVFSILHCGLVTKFILDTEMHTEATEVVQLGPPELLFLLEDLSQKL
ESMLTPPIARRVPFLKNKGSRNIGFSHLQQRSAQEIVHCVFQLLTPMCNH
LENIHNYFQCLAAENHGVVDGPGVKVQEYHIMSSCYQRLLQIFHGLFAWS
GFSQPENQNLLYSALHVLSSRLKQGEHSQPLEELLSQSVHYLQNFHQSIP
SFQCALYLIRLLMVILEKSTASAQNKEKIASLARQFLCRVWPSGDKEKSN
ISNDQLHALLCIYLEHTESILKAIEEIAGVGVPELINSPKDASSSTFPTL
TRHTFVVFFRVMMAELEKTVKKIEPGTAADSQQIHEEKLLYWNMAVRDFS
ILINLIKVFDSHPVLHVCLKYGRLFVEAFLKQCMPLLDFSFRKHREDVLS
LLETFQLDTRLLHHLCGHSKIHQDTRLTQHVPLLKKTLELLVCRVKAMLT
LNNCREAFWLGNLKNRDLQGEEIKSQNSQESTADESEDDMSSQASKSKAT
EDGEEDEVSAGEKEQDSDESYDDSD*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU37129
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Variant 2 Grow at or below 30ºC
• Low Temp: Yes