cDNA Clone C1orf124

• Plasmid: pET43 DU 6xHis C1orf124 445-489
• Parent Plasmid: pET43D 6His
• Expressed: NUS-6xHis-S.TAG-Prec-6xHis-C1orf124 445-489
• Genbank: NM_032018.4
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCATGAGCAGTTCATCCAGTCAGAGCAAAATGGTTAATTGCCCAGTTTGTCAGAATGAAGTTCTGGAGTCTCAAATTAATGAGCACTTGGACTGGTGCCTTGAAGGTGACAGCATCAAAGTCAAAAGCGAAGAAAGTCTTTGAGCGGCCGC

Amino Acid Sequence

MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEIDVRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTFDRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEAVILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGEEVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLWDDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGWELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKELLEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAARGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEATSGSGHHHHHHSAGKETAAAKFERQHMDSPPPTGLVPRGSAGSGTIDDDDKSPGARLEVLFQGPGSHHHHHHGSMSSSSSQSKMVNCPVCQNEVLESQINEHLDWCLEGDSIKVKSEESL*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU37077
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Maintains 6xHIS following cleavage; Bacterial expression with N-ter NUS tag to aid solubility. Maintains N-ter 6xHIS following cleavage; ; Estimated MW of expressed protein : 67739.04 Daltons