cDNA Clone PRPF8

• Plasmid: pET43 DU 6xHis PRPF8 DUB domain 2089-2243
• Parent Plasmid: pET43D
• Expressed: NUS-6xHis-S.TAG-Prec-6xHis-PRPF8 DUB domain 2089-2243
• Genbank: NM_006445.3
• Tag: 6His
• Species: Human

Sequence of Insert

GTCGACATGCACATCTATGTTTCATCTGACGACATCAAGGAGACTGGCTACACCTACATCCTTCCCAAGAATGTGCTTAAGAAGTTCATCTGCATATCTGACCTTCGGGCCCAAATTGCAGGATACCTATATGGGGTGAGCCCACCAGATAACCCCCAGGTGAAGGAGATCCGCTGCATTGTGATGGTGCCGCAGTGGGGCACTCACCAGACCGTGCACCTGCCTGGCCAGCTGCCCCAGCATGAGTACCTCAAGGAGATGGAACCCTTAGGTTGGATCCACACTCAGCCCAATGAGTCCCCGCAGTTATCACCCCAGGATGTCACCACCCATGCCAAGATCATGGCTGACAACCCATCTTGGGATGGCGAGAAGACCATTATCATCACATGCAGCTTCACGCCAGGCTCCTGTACACTGACGGCCTACAAGCTGACCCCCAGTGGCTACGAATGGGGCCGCCAGAACACAGACTGAGCGGCCGC

Amino Acid Sequence

MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEIDVRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTFDRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEAVILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGEEVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLWDDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGWELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKELLEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAARGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEATSGSGHHHHHHSAGKETAAAKFERQHMDSPPPTGLVPRGSAGSGTIDDDDKSPGARLEVLFQGPGSHHHHHHGSSEFEVDMHIYVSSDDIKETGYTYILPKNVLKKFICISDLRAQIAGYLYGVSPPDNPQVKEIRCIVMVPQWGTHQTVHLPGQLPQHEYLKEMEPLGWIHTQPNESPQLSPQDVTTHAKIMADNPSWDGEKTIIITCSFTPGSCTLTAYKLTPSGYEWGRQNTD*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU37032
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Maintains 6xHIS following cleavage; Bacterial expression with N-ter NUS tag to aid solubility. Maintains N-ter 6xHIS following cleavage; ; Estimated MW of expressed protein : 80947.14 Daltons