cDNA Clone SLX4 (mouse)

• Plasmid: pGEX6P-1-mSLX4
• Parent Plasmid: pGEX6P1
• Expressed: GST-mSLX4
• Genbank: NM_177472.4
• Tag: GST
• Species: Mouse

Sequence of Insert

GAATTCATGGTTCCAGAGAGTGCTCCCAATGGCAACTCCCAGCCTCTTCCTTCCTGTTTCACGACAACTGGTGTACCAAGTCCCTCCAAACCCAGAGTCTCAGAGCTGGTTCTGCAACGGATGAAGCAGTTCAAGAGAGCAGACCCTGAGCGCCTGAGACACGCCTCAGAAGAATCCCCCCAAAAGACTGCACTGGGAGACGATGTCCCAAGGAGCCCTCCAGAGGAGACAGTGGGAGAAAATGAGTATAAACTTGATGCCACAGACAGTGATGCTGCAATGGCCCTGGCCTTACAGCAGGAGTTTAGAAGGGAGGAAGCATCCTCACACCATGACAGCCTGGAGGAGAAGGGGTTGTTCTTCTGTCAAATGTGTCAGAAAAACCTCTCAGCAATGAATGTAACCCGGAGGGAGCAACATGTGAACAGATGCCTAGATGAGGCAGAGAAGGCACAGAGACCTGCTTCACCTCGGATCCCTGATTGCCCAATATGTGGGAAACCGTTCCTCACCACCAAGAGCCGAATCAGTCACTTGAAGCAGTGTGCGGTGAGGATGGAAGTGGGTCCCCAACTCCTGCTTCAGGCTGTGAGGCTACAGACAGCTCAGCCAGAGGTGGATGGCAGCCCTCAGGTACCCAGTTTCAGCAACAACGTTGGAGGTTTGAAACGGAAAGGAGTCACCACCAAAAGGGAGCCAAGGAGGAGGAAAGTCAATAAGCCTGAGGCTCCGTCTGAAGATCTGTTGGTGGCCATGGCTCTGTCTCGCTCTGAGGTGGAGCACTGTCCAGTTGTGCCCCCGCTCAGACTGGAAAATGCTTTTTCTGAGAAGATAAGGCTAGGAGCAGAGAAGAAAAGTCGCAAGAAGAGACCCCCGGTGTGTCCCCCACAGTTGGTAACCCAAGACTCTGAGACCACAGGTAGACAGATTGAAGATCGTGTTGCCCAGCTTCTGTCAGAGGAAGCAGAACTATCTTGCACCCCACCACTTCTTGCCAGCAAGATTTCTAAGGAAGAATTGGAACCTGCAGGCTGGCGAGCACGGCTGCCTGAAGGGAAGCGGAACTTTCTGTGGGAACTCAGCGCTCTCACAGGAGCCTGGGCTGAGGAGTCCTTCTACACAGTGGGACTGTTTCCTCCAATTGTGTCCCAATGCCCTTCCAAGGAACCCCAGCTACCATTGGAGCTGCCCAAGCAAGGAGAGCCGAGTCCACGGCGGCCACCTGCTTCCCAGAGCAGCCTTCCTGTGAGCCACAGCCCCAAGATCCGGCTGCTGTCCTCCAGCCAGAGGGAGCGTCAAGCCCTTCAGGACCTTGTGGACCTGGCAGTGGAAGGGCTGAGCTCTAGCCCACAGCCCGGGAGTCGGGGCGTACCCACAGGGTTGGACCTGGTACCTAGCAGCCTTCCGCTGACGGGGTTTGTCCTTCCATGTAAGAAGACCCTGAAGAAGGATGACAGTGCCTCACTCTCTCTTGGGTTATTGGTGACAGACTTTGGGGCCATGGTCAATAATCCACACCTGAGTGATGTCCAGTTCCAGCTTGACAGTGGAGAGGTGCTTTATGCACACAAGTTTGTTCTTTATGCGAGATGCCCACTTCTTATTCAGTATGTAAGCACTGAAAGCTTTTCTTCTGAAGAGGACGGGGATCTGACCCAGCGTGCTCTGCTGAGTGATGTGAGCTCGGAGGCCGCCCATGCATTTCTGAACTACCTCTACATGGCAGACACTGACATGCCTCCCAGCCTGGTTCCGGATCTGCGTTCCCTGGCCCTAAGGTTTGGTGTGAGTGACCTTGTTCAGCTGTGTGAACAAGTGCCCGCCGTGGTGGACTTAGAGGGAGAACAGCCAGAAGAGACCAGTGAGGACTGTGAAAGCCGAGCAGAGACTTTCCTGGAACTCCTGAGGTCGGTGTGGGTAGACAATGAGGAGGAAGTGGAGACTCTGCTGAAACCTGAGCTCTGTGAAGAAGAGAGAGAAAGAGTGAATGAAGCAGAAATGGAAGAGATATATGAATTTGCTGCTACGCAGAGGAAGCTTCTCCAGTGGGGGAGAGCAGCAGACCCAGATGGGAGCACTAACCCGCATGGGGAAGACGGTGCAGTTTCTGAGCCTTCCCTGGCAGGTGTCCAGAGTAACAGACAGCTAGAAAATACAGAGCACATGGAGTCATCTGGGTTAGAAAAAGAAGAGGCACTAGCCAGCTGGGAACAAGAAGGACATTCCACTCCACTGCAGGACCAGTGCCCAGACTGGGCAGGGAAAGCAGAGGCCCAGGATGCATTGGGGGAGGCAACTGACGACCCCAGCTTCTGCAGCCGCCACAGGAGGGGGAAAGAGTGCTTGCCTTTGCACCCAAACAAGGCCCATGGCTGCAAACAGCCCCTTCCATCAAATCCAAGAGTGTCATCTGAACTGTCACAAATAACAGTTGATCATGAAGAGCAGAGTGACCATGTCAGGGAGACGCAGGCAGACATGGCCCAGGCACCTACTCCACACTCCTGCAGCCTTGTGTCACAGTCTTCTGTGGATGGAAGTCCCAGCCAGTCATGGCTTCACCTATACCACACTAGTCATTTGTCCCCCTCAGTGTCCCAGTCACACAGTAGCATTTCCAGGGTGGCATCCCCAAGATCACTGTCTCCAACTACACCAACTAAGCAGAGGAGGGGCAGTAACATTGTCACATTACGGAAAGATGCTGGCCACCACAGAGGCCAACAAAGTAGCCCCATAGCGGGACACAGAAATAGGGGCATCCTGATTTCCCCAGCAAAGTCTCCACCCATTGACCTCACACAGTCAGTACCCGAGCCCCTGAGCCCCAGGGCCCAGGATCCTCTGCATTTCGTGAAAAAGGAGGATGAGGTCATCCTGTTACTGGACTCAGATGAGGAGCTGGAGCACACCAAGACAGAGTCAGTTTCTAAAGATTCCCCAGAAGGAAGGAAAGTTCCAGAATTTAGCCCCAGGTCCTCTGAACTGTTTTCAGTCATTGATGTTGAAGAAGATCATGAACATTTCCAAAGCCCACTGAAGAGAGAGGCCGGGCTACAGCACGGGGAGGAAGGACAGCTGGGAAACCAGTCTGCGCTGGGCTGCAGAGACATCCCCTGGCTGCTCTGCAGCCAGAAAACCAGCCTGGATGAGGACAGTGCTACAGACACCTCTTGGCTGGTGCCTGCCACTCCAGGGGTCAGCAGGAGTCGTGATTGCTCATCACAGACCCAAATCAAAAGCCTTAAGACCAGGATTCCGTCAGATGAGACTGCTCAGCAAACACCTAGACCTAACTTAGAACGCAGGACCATGCTGGAAACAGCCCAGCAGTTCTCTGTGATCATGCCACACACACAGCCTATTACTCTAGGAGCCTTTGACAGCGGAAGACAGGCCTACAGAAGCCCTTCCCATCCCTACCCCAGGCACCACAGACTCTCTTCTTCACAGCCATCATGTCCTGGCCCTGATTTTACCAGGTGGTCCCAGAAATCTTCCGCACCTAGACCATGCCTGCCAAATCTGCCTGCAGCTGATGATGTGGTAGAAGTTGGGGACAGTGATGATGAGGTGGCTTCCCATCAAGGAAACAGCAGCCCCGTGCTAGATGGTGACCCCCCAGGTCCCATGGGTGACTACTGTTGGAATGAGCCTCTCTCACCAATTCCAATTGACCACTTGAATCTGGAGCGGACCGGCCCCCTGACCACAAGCAGTCCCAGCAGTCAGGTCCTGGAGGCTTTGCACAGTGATGACTGCCACTCCCCGGGACTTGGTACCACCCCCATCCGAGGAAGCTGTGGTACGCTGAGAGAGTCTCAGGAACGGTCCTCACTGGCTGGCTCTCCAGAGGCTCTGTGGGACGACTGGAATGAGGAGGAAGGACAGTCTCCAGAGGCCCCACCTGTGGCTCAAATGCTGAGTACCAGAACTCGGAAGCCAGACAGGCCAGAGACACCAAAAGGTGCTAACCAGAAAAAGAACTTGCCTCCCAAAGTGCCTATAACTCCAATGCCAAGGTATTCGATCATGGAGACCCCGGTGCTGAAGAAAGAACTGGACAGGTTTGGAGTCCGTGCTCTGCCCAAACGCCAGATGGTTCTAAAACTGAAGGAGATTTTCCAGTACACTCACCAGACTCTGGAGTCGGACTCTGAGGATGAGGTCCAGTCCCCCCAGATACCCGCAGAACTACCTTGTCGCCAGGCATCTACCACTGAGACCTGCAACCCTTCAAGGTTGCCAACTGGGGAGCCTTCACATCCTGATGGTGACGCCCAGCTCCCAGCCTCCCAGGAATCCATGGCTACCTCTGTGGACGGCAGCGACAACTCCTTTAGCTCTAAAAGCTCCTCTGCTGAGTTTGGAGCTGCGTTTGAGTATTCAGATGAAGACAAGGATGAGGAGGTCGGGGTCACTGCATCACAGGCCGCAATCCAGGCCGCAGACACAGAGGAGGCAGTGAGGCGCTATATCCGCTCTAAGCCTGCCCTGCACAGGCAGGTCCTGAGGTACCAGCCTGTGGAGCTGGCCGAGCTGCAGGCTGAGCTGAAACAGAATGGCATCCCTGTGGCCATGGGCAAGCTGTCGGACATCCTGGACGCTCAGTGCATCACCTTCACCACTGCTGCAGCCCGAAAGGAGAAACTCAAACACAAGAGACGGCAGCCCTCAGGCCGAAAGAAAAAGGATCAGAAATGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPEFMVPESAPNGNSQPLPSCFTTTGVPSPSKPRVSELVLQRMKQFKRADPERLRHASEESPQKTALGDDVPRSPPEETVGENEYKLDATDSDAAMALALQQEFRREEASSHHDSLEEKGLFFCQMCQKNLSAMNVTRREQHVNRCLDEAEKAQRPASPRIPDCPICGKPFLTTKSRISHLKQCAVRMEVGPQLLLQAVRLQTAQPEVDGSPQVPSFSNNVGGLKRKGVTTKREPRRRKVNKPEAPSEDLLVAMALSRSEVEHCPVVPPLRLENAFSEKIRLGAEKKSRKKRPPVCPPQLVTQDSETTGRQIEDRVAQLLSEEAELSCTPPLLASKISKEELEPAGWRARLPEGKRNFLWELSALTGAWAEESFYTVGLFPPIVSQCPSKEPQLPLELPKQGEPSPRRPPASQSSLPVSHSPKIRLLSSSQRERQALQDLVDLAVEGLSSSPQPGSRGVPTGLDLVPSSLPLTGFVLPCKKTLKKDDSASLSLGLLVTDFGAMVNNPHLSDVQFQLDSGEVLYAHKFVLYARCPLLIQYVSTESFSSEEDGDLTQRALLSDVSSEAAHAFLNYLYMADTDMPPSLVPDLRSLALRFGVSDLVQLCEQVPAVVDLEGEQPEETSEDCESRAETFLELLRSVWVDNEEEVETLLKPELCEEERERVNEAEMEEIYEFAATQRKLLQWGRAADPDGSTNPHGEDGAVSEPSLAGVQSNRQLENTEHMESSGLEKEEALASWEQEGHSTPLQDQCPDWAGKAEAQDALGEATDDPSFCSRHRRGKECLPLHPNKAHGCKQPLPSNPRVSSELSQITVDHEEQSDHVRETQADMAQAPTPHSCSLVSQSSVDGSPSQSWLHLYHTSHLSPSVSQSHSSISRVASPRSLSPTTPTKQRRGSNIVTLRKDAGHHRGQQSSPIAGHRNRGILISPAKSPPIDLTQSVPEPLSPRAQDPLHFVKKEDEVILLLDSDEELEHTKTESVSKDSPEGRKVPEFSPRSSELFSVIDVEEDHEHFQSPLKREAGLQHGEEGQLGNQSALGCRDIPWLLCSQKTSLDEDSATDTSWLVPATPGVSRSRDCSSQTQIKSLKTRIPSDETAQQTPRPNLERRTMLETAQQFSVIMPHTQPITLGAFDSGRQAYRSPSHPYPRHHRLSSSQPSCPGPDFTRWSQKSSAPRPCLPNLPAADDVVEVGDSDDEVASHQGNSSPVLDGDPPGPMGDYCWNEPLSPIPIDHLNLERTGPLTTSSPSSQVLEALHSDDCHSPGLGTTPIRGSCGTLRESQERSSLAGSPEALWDDWNEEEGQSPEAPPVAQMLSTRTRKPDRPETPKGANQKKNLPPKVPITPMPRYSIMETPVLKKELDRFGVRALPKRQMVLKLKEIFQYTHQTLESDSEDEVQSPQIPAELPCRQASTTETCNPSRLPTGEPSHPDGDAQLPASQESMATSVDGSDNSFSSKSSSAEFGAAFEYSDEDKDEEVGVTASQAAIQAADTEEAVRRYIRSKPALHRQVLRYQPVELAELQAELKQNGIPVAMGKLSDILDAQCITFTTAAARKEKLKHKRRQPSGRKKKDQK*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU33988
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion ; ; Estimated MW of expressed protein : 199609.58 Daltons; ; All BTBD12 (SLX4) plasmids MUST be grown at 30 degrees C or less to prevent recombination.