cDNA Clone FAN1 (C.elegans)

• Plasmid: pET43 DU FAN1 C.elegans
• Parent Plasmid: pET43a +3C
• Expressed: NUS-6xHis-S.TAG-Prec-FAN1 C.elegans
• Genbank: NM_068596.3
• Tag: 6His
• Species: Caenorhabditis elegans

Sequence of Insert

GGATCCATGAAGAAAGCCAAGAAAGAGAAGAAAGAAAAGATCATTAAGCCAGACGGACCCATTAACTTTAATCGGAGTATCATGGCCGCTTTCGAGAAACAGAGCCGGGGCAAGATTTGCCCTCTGTGCGAAACTAAATTTTCACTGGCCAGCTACAAGTCCCACATGAACACCTGCAATGTGGCTGACGATGACGAGGAAATCGAAGTGATTGCCACATATACTCGCGACGAGGCTATCCTGATGAGGGCTGGACCAGAAATCATTCTGGGGGATGCCTCCTTCTCTGACAAGAGCGAGAACCCCACCAAACGGAGAAAGACAGATGAGCGGGAAGTGCCATCAGAGGATGACATTGTGCCCGAAGTGCCCGGACCTAGCGGCATCGTGAAGAACCACGAGATGCCTAGTGAATCACTGGACGTGACCGAGATCAGCGAAAATATTGAGAAAGTGATCAAGAAGAGCCCAGAGTGGATCAACCATCGGCGGCGGAGCAGCCGGCTGCTCCAGAACAGCCAGAAAGACCAGGCCGATAACGCTAATAAGGAGGACCCAGTGAAGAAAGAAACCGCCACAATTTCTGAGGTGCTCCAGGCTATCGAACGCTTCGAGCAGCGGGTGAGTGGACCAGAGCAGACATGGCCTTACTACATCAAAATCACTATCAAAATCATGAAGAGAGTGATCAGCACTGAGAAGTTCGACGGGACCTTTTACGCCGATGACTTTTGGCTGCCCTCCGATATCATTACATTCTATCGCTTTGTGGAGCTGCTGTCTGAAGGAGCTAAATGTCTGCTGGTGAGACTGTTCATCAGGAAGCCTGCCTGGTACAACCTGGAAAAACTGGAGCAGAAGTATCCTGAGATCCCAAATATCAAGGAGGCCGTGTCCGAACTGGCTAAGGGACACTTCATCGATGACGATTCTAGTATGAAAACCCTGGATGAGGCCCTCCAGATCAGCGACGTGGTGGCTCTGAAGAACGTGACCAAGAAATTCAAACTGGATGGCACAAAGAATCGCCAGGAGCTGATCCAGTCCCTGCGGAAATTTGCCCAGTCTCAGCAGAGTATTTTCGGCGGGACCGGCAACGTGGAGAAGAGCATCCTGAAATCCCTGAAGCAGGAACTGGGACCATGCGTGAGAGTGAGGGGAGGATTCGTGGACCTGTTTAAGTGCCTGTTCACAATCTACTGTCCCGTGACCACAAACTCAGCCAATGTGATCGACAACCCTAGCACTACCAACGTGTACCAGGATCTGCTGTATCTGATGCTGTCCGTGGCCAACGGGACAGTGCAGTTTCCAGCTCCCAATCCTTGTCCAATCATTGCCTCTTTCTACAAAAACAGGAATATGCTCCAGGATTATATGATCAGCAAGTCCCTGGAGATCGCCATCGTGAGCCAGATGAGCAACGGAAATCTGGACGCCGCTCTGGATCTGGCCATTGACGCTAAAGAGTTCATCGAGCAGATGAGCGACGATGACAAGAGATACTATGAATCCCTGGAGATTCACGAAAGGAAGTTTACAAGCATCTGGGTGTTCACTCGCTGCTGTGGGCATGCTTCAAGCATCCTGGAGCGGCAGAAGAAATACGGAATGGCCGTGGAATGGCAGAAGGACCTGCTGATTACCAACAAGGACATCCAGTCTTATTGCATTGATAGTCGCGGCATCTGGTGGGACCGGATGCTGCTGAATCTGGATAGTCACCTGAAAGAGAAGAAAGAATGTGCCAAGATGATCCAGATCGCCCTCCAGGACCCCTCTATTCTGGAGAAGGAACTGCTGATGATCCAGGACAGGGCTCTGAAGCTGAAAGAGATGCCAGCCGATTTCGTGACACCCATCAACATTGGGAATCCTGAAAAGAAAACTATTACCGCCAACGTGATCACTAAGAGCCTGGGGGACGGACGCATTAATCGGTTTATGATCCGCGATCACGAAACTGATGACGATGTGGAGTGCAGCGTGGAGGAAGTGACCAGAAGGCATTACCTGGAGAACGAAGGGTTCTCCACAGGAGTGCACGATGAGGGATCTACATGGCATACTCTGTTTGGCCTGTTCTTTTATGATGTGATCTTCGCCACTGACGAGTCCGTGGAATCTACCTGGCTGTCAGAGCTGCAAGATTGCCCAAGTGACCTGTCAAACACTCTGTACAGCAAGAGAAAAGAGAAGTTTGAAGACAGGTTCGTGTGGCTGGAGGAAGCTGAGCAGGAACTGATCGAGGAAAACATCAGGAAAATTTGGGACCTGAAGCACAACGAGACCAATCGCGAATGTAGTTGGAAGCAGTTCCCTATGGGCGCCGAGGATTGCGTGTCATTCTTTCAGTGTATCCCCCGGCCTGCTCTGATCCTGATTCTGCGCCGGCTGGCCGAGAACTATAGAAATTCTAGGAGTGGGTTTCCTGACCTGACCCTGTGGAACCCAGAAACAAAAAGAGTGGCTGTGGTGGAGGTGAAGGGACCAGGGGACAGACTGAGCACCAAGCAGAGGCTGTGGCTGGCCATTTTCGCTGATTCCGGCATCCGGGCTGAGGTGTGCCATGTGGCTGCTCAGAACAGCCGGCTGCTGGTGTGAGCGGCCGC

Amino Acid Sequence

MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEIDVRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTFDRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEAVILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGEEVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLWDDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGWELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKELLEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAARGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEATSGSGHHHHHHSAGKETAAAKFERQHMDSPPPTGLVPRGSAGSGTIDDDDKSPGARGSMKKAKKEKKEKIIKPDGPINFNRSIMAAFEKQSRGKICPLCETKFSLASYKSHMNTCNVADDDEEIEVIATYTRDEAILMRAGPEIILGDASFSDKSENPTKRRKTDEREVPSEDDIVPEVPGPSGIVKNHEMPSESLDVTEISENIEKVIKKSPEWINHRRRSSRLLQNSQKDQADNANKEDPVKKETATISEVLQAIERFEQRVSGPEQTWPYYIKITIKIMKRVISTEKFDGTFYADDFWLPSDIITFYRFVELLSEGAKCLLVRLFIRKPAWYNLEKLEQKYPEIPNIKEAVSELAKGHFIDDDSSMKTLDEALQISDVVALKNVTKKFKLDGTKNRQELIQSLRKFAQSQQSIFGGTGNVEKSILKSLKQELGPCVRVRGGFVDLFKCLFTIYCPVTTNSANVIDNPSTTNVYQDLLYLMLSVANGTVQFPAPNPCPIIASFYKNRNMLQDYMISKSLEIAIVSQMSNGNLDAALDLAIDAKEFIEQMSDDDKRYYESLEIHERKFTSIWVFTRCCGHASSILERQKKYGMAVEWQKDLLITNKDIQSYCIDSRGIWWDRMLLNLDSHLKEKKECAKMIQIALQDPSILEKELLMIQDRALKLKEMPADFVTPINIGNPEKKTITANVITKSLGDGRINRFMIRDHETDDDVECSVEEVTRRHYLENEGFSTGVHDEGSTWHTLFGLFFYDVIFATDESVESTWLSELQDCPSDLSNTLYSKRKEKFEDRFVWLEEAEQELIEENIRKIWDLKHNETNRECSWKQFPMGAEDCVSFFQCIPRPALILILRRLAENYRNSRSGFPDLTLWNPETKRVAVVEVKGPGDRLSTKQRLWLAIFADSGIRAEVCHVAAQNSRLLV*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU33675
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression with N-ter NUS tag to aid solubility; ; Estimated MW of expressed protein : 160165.04 Daltons; ; All MTMR15 (FAN1) plasmids MUST be grown at 30 degrees C or less to prevent recombination.