cDNA Clone HYOU1

• Plasmid: pcDNA5-FRT/TO-GFP HYOU1
• Parent Plasmid: pcDNA5 FRT/TO GFP
• Expressed: GFP-HYOU1
• Genbank: NM_006389.3
• Tag: GFP
• Species: Human

Sequence of Insert

GGATCCATGGCAGACAAAGTTAGGAGGCAGAGGCCGAGGAGGCGAGTCTGTTGGGCCTTGGTGGCTGTGCTCTTGGCAGACCTGTTGGCACTGAGTGATACACTGGCAGTGATGTCTGTGGACCTGGGCAGTGAGTCCATGAAGGTGGCCATTGTCAAACCTGGAGTGCCCATGGAAATTGTCTTGAATAAGGAATCTCGGAGGAAAACACCGGTGATCGTGACCCTGAAAGAAAATGAAAGATTCTTTGGAGACAGTGCAGCAAGCATGGCGATTAAGAATCCAAAGGCTACGCTACGTTACTTCCAGCACCTCCTGGGGAAGCAGGCAGATAACCCCCATGTAGCTCTTTACCAGGCCCGCTTCCCGGAGCACGAGCTGACTTTCGACCCACAGAGGCAGACTGTGCACTTTCAGATCAGCTCGCAGCTGCAGTTCTCACCTGAGGAAGTGTTGGGCATGGTTCTCAATTATTCTCGTTCTCTAGCTGAAGATTTTGCAGAGCAGCCCATCAAGGATGCAGTGATCACCGTGCCAGTCTTCTTCAATCAGGCCGAGCGCCGAGCTGTGCTGCAGGCTGCTCGTATGGCTGGCCTCAAAGTGCTGCAGCTCATCAATGACAACACCGCCACTGCCCTCAGCTATGGTGTCTTCCGCCGGAAAGATATTAACACCACTGCCCAGAATATCATGTTCTATGACATGGGCTCAGGCAGCACCGTATGCACCATTGTGACCTACCAGATGGTGAAGACTAAGGAAGCTGGGATGCAGCCACAGCTGCAGATCCGGGGAGTAGGATTTGACCGTACCCTGGGGGGCCTGGAGATGGAGCTCCGGCTTCGAGAACGCCTGGCTGGGCTTTTCAATGAGCAGCGCAAGGGTCAGAGAGCAAAGGATGTGCGGGAGAACCCGCGTGCCATGGCCAAGCTGCTGCGTGAGGCTAATCGGCTCAAAACCGTCCTCAGTGCCAACGCTGACCACATGGCACAGATTGAAGGCCTGATGGATGATGTGGACTTCAAGGCAAAAGTGACTCGTGTGGAATTTGAGGAGTTGTGTGCAGACTTGTTTGAGCGGGTGCCTGGGCCTGTACAGCAGGCCCTCCAGAGTGCCGAAATGAGTCTGGATGAGATTGAGCAGGTGATCCTGGTGGGTGGGGCCACTCGGGTCCCCAGAGTTCAGGAGGTGCTGCTGAAGGCCGTGGGCAAGGAGGAGCTGGGGAAGAACATCAATGCAGATGAAGCAGCCGCCATGGGGGCAGTGTACCAGGCAGCTGCGCTCAGCAAAGCCTTTAAAGTGAAGCCATTTGTCGTCCGAGATGCAGTGGTCTACCCCATCCTGGTGGAGTTCACGAGGGAGGTGGAGGAGGAGCCTGGGATTCACAGCCTGAAGCACAATAAACGGGTACTCTTCTCTCGGATGGGGCCCTACCCTCAACGCAAAGTCATCACCTTTAACCGCTACAGCCATGATTTCAACTTCCACATCAACTACGGCGACCTGGGCTTCCTGGGGCCTGAAGATCTTCGGGTATTTGGCTCCCAGAATCTGACCACAGTGAAGCTAAAAGGGGTGGGTGACAGCTTCAAGAAGTATCCTGACTACGAGTCCAAGGGCATCAAGGCTCACTTCAACCTGGATGAGAGTGGCGTGCTCAGTCTAGACAGGGTGGAGTCTGTATTTGAGACACTGGTAGAGGACAGCGCAGAAGAGGAATCTACTCTCACCAAACTTGGCAACACCATTTCCAGCCTGTTTGGAGGCGGTACCACACCAGATGCCAAGGAGAATGGTACTGATACTGTCCAGGAGGAAGAGGAGAGCCCTGCAGAGGGGAGCAAGGACGAGCCTGGGGAGCAGGTGGAGCTCAAGGAGGAAGCTGAGGCCCCAGTGGAGGATGGCTCTCAGCCCCCACCCCCTGAACCTAAGGGAGATGCAACCCCTGAGGGAGAAAAGGCCACAGAAAAAGAAAATGGGGACAAGTCTGAGGCCCAGAAACCCAGTGTCTGCAGAGCCTCAGTTACCAGGTTTGGTTTCTGAGCGGCCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSMADKVRRQRPRRRVCWALVAVLLADLLALSDTLAVMSVDLGSESMKVAIVKPGVPMEIVLNKESRRKTPVIVTLKENERFFGDSAASMAIKNPKATLRYFQHLLGKQADNPHVALYQARFPEHELTFDPQRQTVHFQISSQLQFSPEEVLGMVLNYSRSLAEDFAEQPIKDAVITVPVFFNQAERRAVLQAARMAGLKVLQLINDNTATALSYGVFRRKDINTTAQNIMFYDMGSGSTVCTIVTYQMVKTKEAGMQPQLQIRGVGFDRTLGGLEMELRLRERLAGLFNEQRKGQRAKDVRENPRAMAKLLREANRLKTVLSANADHMAQIEGLMDDVDFKAKVTRVEFEELCADLFERVPGPVQQALQSAEMSLDEIEQVILVGGATRVPRVQEVLLKAVGKEELGKNINADEAAAMGAVYQAAALSKAFKVKPFVVRDAVVYPILVEFTREVEEEPGIHSLKHNKRVLFSRMGPYPQRKVITFNRYSHDFNFHINYGDLGFLGPEDLRVFGSQNLTTVKLKGVGDSFKKYPDYESKGIKAHFNLDESGVLSLDRVESVFETLVEDSAEEESTLTKLGNTISSLFGGGTTPDAKENGTDTVQEEEESPAEGSKDEPGEQVELKEEAEAPVEDGSQPPPPEPKGDATPEGEKATEKENGDKSEAQKPSVCRASVTRFGF*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU33364
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression of protein with N-ter GFP tag; ; Estimated MW of expressed protein : 102668.81 Daltons