cDNA Clone KIAA0182

• Plasmid: pCMV5-HA KIAA0182
• Parent Plasmid: pCMV5 HA1
• Expressed: HA-KIAA0182
• Genbank: NM_014615.2
• Tag: HA
• Species: Human

Sequence of Insert

GGATCCATGGGCCCTATCATCGTCCCCCCTGGGGGCCACAGCGTGCCCAGCACCCCCCCCGTGGTGACCATCGCTCCAACCAAAACCGTGAATGGTGTCTGGAGGAGTGAGAGCCGGCAGGATGCCGGCTCCAGGAGCAGCAGTGGAGGTCGGGAACGCCTCATTGTGGAGCCCCCGCTCCCTCAGGAGAAGGCAGGGGGACCAGCCATCCCCTCGCACCTGCTCAGCACCCCCTACCCCTTCGGCCTCTCCCCCAGCTCAGTTGTGCAGGATTCCCGCTTCCCGCCACTCAACCTCCAGCGGCCCGTGCACCACGTGGTGCCCCCCAGTACCGTGACCGAGGACTACCTGAGAAGCTTCCGGCCCTACCACACCACCGACGACCTCCGCATGTCCTCACTGCCTCCCCTCGGCCTGGACCCGGCCACTGCTGCAGCCTACTACCACCCCAGCTACCTGGCCCCACACCCCTTCCCCCACCCGGCCTTCAGGATGGACGACTCCTACTGCCTGTCTGCCCTGAGGTCCCCGTTCTACCCCATCCCCACCCCCGGCTCCCTGCCCCCACTGCACCCATCAGCGATGCACCTGCACCTCTCTGGGGTCCGCTACCCTCCCGAGCTCTCCCACTCATCCCTGGCAGCGCTGCACTCGGAGCGCATGTCTGGCCTCAGCGCGGAGAGGCTGCAGATGGACGAGGAGCTAAGGCGGGAGAGGGAGCGCGAGCGCGAGCGCGAGCGTGAGCGTGAGGCTGACCGCGAGCGGGAGAAGGAACGTGAGCGCGAACGCGAGAAGGAGCGCGAGCAAGAGAAGGAGCGTGAGCGTGAGAAGGAGCGCGAGCGCGAGCTGGAGCGCCAGCGGGAGCAGCGGGCCCGGGAGAAGGAGCTGCTGGCCGCCAAGGCCCTGGAGCCCAGCTTCCTGCCCGTGGCCGAGCTGCATGGGCTGCGTGGCCATGCCACTGAGGAGCGGGGCAAGCCCTCGGAGCAGCTGACCCCAACCCGAGCAGAGAAGCTGAAGGATGCCGGCCTGCAGGCGCCCAAGCCCGTCCAACACCCCTTGCATCCGGTGCCCACCCCACACCACACGGTGCCCAGCCTCATCTCCAACCATGGCATCTTCTCTCTGCCTAGCAGCAGTGCTGCCACAGCCCTGCTGATCCAGCGCACCAATGAGGAGGAGAAGTGGCTGGCGCGGCAGCGGCGGCTGCGGCAGGAGAAGGAGGACCGGCAGTCTCAGGTGTCCGAGTTCCGGCAGCAGGTGCTGGAGCAGCACCTGGATATGGGCCGGCCCCCGGTGCCGGCGGAGGCAGAGCACAGGCCGGAGAGCACCACCAGGCCAGGACCAAACCGTCACGAGCCAGGTGGCCGTGACCCTCCGCAGCACTTTGGGGGGCCACCACCTCTGATTTCGCCCAAGCCCCAGCTCCATGCTGCACCCACGGCCCTCTGGAACCCCGTGTCCCTGATGGACAACACCTTGGAGACGCGGCGGGCCGAAAGCCACTCTCTGCACAGCCACCCGGCTGCATTTGAGCCCAGCCGCCAGGCAGCCGTGCCGCTGGTGAAGGTGGAGCGGGTCTTCTGCCCGGAGAAAGCAGAGGAGGGGCCACGGAAGCGTGAGCCTGCCCCTCTGGACAAGTACCAGCCACCTCCGCCGCCACCACGAGAGGGAGGGAGCCTGGAGCACCAGCCCTTCCTGCCCGGGCCCGGGCCCTTCCTGGCTGAGCTCGAGAAGTCCACCCAGACCATCCTGGGCCAGCAGCGGGCCTCCCTCCCACAGGCGGCCACCTTCGGGGAGCTCAGCGGACCCCTGAAGCCTGGCTCGCCCTACCGGCCCCCAGTGCCACGGGCCCCCGACCCTGCCTACATCTATGATGAGTTCCTGCAGCAGCGCCGGAGGCTGGTCAGCAAGCTGGACCTGGAGGAGCGCAGGCGGCGGGAGGCCCAGGAGAAAGGGTACTACTACGACCTCGATGACTCTTACGACGAGAGCGATGAGGAGGAGGTCAGGGCCCACCTCCGTTGCGTGGCCGAGCAGCCGCCCCTCAAACTGGACACGTCCTCTGAGAAGCTAGAGTTTTTGCAACTTTTTGGCTTGACCACCCAACAGCAGAAGGAGGAATTGGTGGCCCAGAAGCGGAGGAAGCGGCGGAGGATGCTGCGAGAGAGAAGCCCGTCGCCCCCAACAATTCAGAGCAAGCGGCAGACGCCTTCACCGAGACTGGCGCTGTCTACCCGCTACAGCCCTGATGAGATGAACAACAGTCCCAACTTCGAAGAAAAGAAGAAGTTCCTGACCATCTTCAACCTGACCCACATCAGCGCTGAGAAGAGGAAAGACAAAGAGAGACTTGTTGAAATGCTCCGTGCCATGAAGCAGAAGGCACTGTCAGCAGCAGTGGCCGACTCCTTGACAAACTCTCCGAGGGACAGTCCTGCCGTCTCCCTGAGTGAACCAGCCACGCAGCAAGCCTCTCTGGATGTGGAGAAGCCGGTTGGTGCTGCTGCTTCCTTGTCTGACATCCCAAAGGCCGCGGAGCCTGGGAAGCTGGAACAGGTCCGGCCCCAGGAGCTGTCGAGAGTCCAGGAGCTAGCTCCTGCCAGCGGGGAGAAGGCCAGGCTGAGCGAGGCCCCTGGAGGCAAAAAGAGTCTGAGCATGCTTCACTATATCCGGGGCGCTGCACCCAAGGACATTCCTGTGCCGCTGTCCCACAGCACCAATGGGAAGAGCAAGCCGTGGGAGCCCTTTGTGGCAGAAGAGTTTGCACATCAGTTCCACGAGTCAGTGCTGCAGTCCACCCAGAAGGCCCTGCAGAAGCATAAAGGGAGCGTGGCTGTGCTGTCTGCAGAGCAGAACCACAAGGTTGACACGTCCGTCCACTACAACATTCCTGAGCTGCAGTCCTCCAGCCGCGCCCCTCCACCCCAGCACAATGGGCAGCAGGAGCCCCCCACTGCAAGGAAGGGCCCCCCAACCCAGGAGTTGGACCGGGACTCGGAGGAGGAGGAAGAGGAGGATGATGAAGATGGAGAAGATGAGGAGGAAGTCCCCAAGCGCAAGTGGCAAGGGATCGAGGCCGTTTTTGAAGCTTACCAGGAACACATAGAAGAGCAAAATCTGGAGCGGCAGGTGTTACAGACACAATGTAGACGACTGGAGGCCCGGCACTACAGCCTCAGCCTGACGGCAGAGCAGCTCTCCCACAGCGTGGCGGAGTTGAGGAGCCAGAAACAGAAGATGGTCTCAGAAAGGGAGCGGCTCCAGGCAGAACTGGACCACTTACGAAAGTGCCTTGCCTTGCCTGCAATGCACTGGCCTAGGGGCTACCTGAAGGGATATCCCAGGTGAGCGGCCGC

Amino Acid Sequence

MYPYDVPDYAGSMGPIIVPPGGHSVPSTPPVVTIAPTKTVNGVWRSESRQDAGSRSSSGGRERLIVEPPLPQEKAGGPAIPSHLLSTPYPFGLSPSSVVQDSRFPPLNLQRPVHHVVPPSTVTEDYLRSFRPYHTTDDLRMSSLPPLGLDPATAAAYYHPSYLAPHPFPHPAFRMDDSYCLSALRSPFYPIPTPGSLPPLHPSAMHLHLSGVRYPPELSHSSLAALHSERMSGLSAERLQMDEELRREREREREREREREADREREKEREREREKEREQEKEREREKERERELERQREQRAREKELLAAKALEPSFLPVAELHGLRGHATEERGKPSEQLTPTRAEKLKDAGLQAPKPVQHPLHPVPTPHHTVPSLISNHGIFSLPSSSAATALLIQRTNEEEKWLARQRRLRQEKEDRQSQVSEFRQQVLEQHLDMGRPPVPAEAEHRPESTTRPGPNRHEPGGRDPPQHFGGPPPLISPKPQLHAAPTALWNPVSLMDNTLETRRAESHSLHSHPAAFEPSRQAAVPLVKVERVFCPEKAEEGPRKREPAPLDKYQPPPPPPREGGSLEHQPFLPGPGPFLAELEKSTQTILGQQRASLPQAATFGELSGPLKPGSPYRPPVPRAPDPAYIYDEFLQQRRRLVSKLDLEERRRREAQEKGYYYDLDDSYDESDEEEVRAHLRCVAEQPPLKLDTSSEKLEFLQLFGLTTQQQKEELVAQKRRKRRRMLRERSPSPPTIQSKRQTPSPRLALSTRYSPDEMNNSPNFEEKKKFLTIFNLTHISAEKRKDKERLVEMLRAMKQKALSAAVADSLTNSPRDSPAVSLSEPATQQASLDVEKPVGAAASLSDIPKAAEPGKLEQVRPQELSRVQELAPASGEKARLSEAPGGKKSLSMLHYIRGAAPKDIPVPLSHSTNGKSKPWEPFVAEEFAHQFHESVLQSTQKALQKHKGSVAVLSAEQNHKVDTSVHYNIPELQSSSRAPPPQHNGQQEPPTARKGPPTQELDRDSEEEEEEDDEDGEDEEEVPKRKWQGIEAVFEAYQEHIEEQNLERQVLQTQCRRLEARHYSLSLTAEQLSHSVAELRSQKQKMVSERERLQAELDHLRKCLALPAMHWPRGYLKGYPR*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU33215
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N-ter HA mammalian expression of protein of interest; ; Estimated MW of expressed protein : 127173.93 Daltons