cDNA Clone FAN1 (Pseudomonas)

• Plasmid: pET43A FAN1p
• Parent Plasmid: pET43a +3C
• Expressed: NUS-6xHis-S.TAG-FAN1p
• Genbank: AE004091.2
• Tag: 6His
• Species: Pseudomonas

Sequence of Insert

GGATCCATGCATGAACAGTACCAGGCTCCGCTTCCCGTGAATTCCCCAGCGCTTCCCGAACCCTTCTACTACCTGCACAACTTCCGCGCGGTGCTCGCCTGGATCGGCGAACGCTATGCCGATCTGCTCGACGATCAGGAACGTGCCTTCATCGCCGCGTTCGCCGAGCTGCCCGAGGCCAGCCAGGCCCTGCTGGTGCGCATGGTGATGCGCAAGGGCACGCTGTTCCGCGAAGGCAAGCTGGCCTATGCCGAGATCGGCGACACGCGCGCCGCCGTGCAACCGCTGTTGGCATTGGGCTGGGTGGACGCGCAGCCGACCCTGGAACTGGCGCAACTGTTCGGCTTGCTGAAGAAGGACGAATTGTCGCAGCTGTTCAGGGACCATCTCGGGCGCGCGAACCTGCGCAAGGATGCGCTGCTGGAACGGCTCCAGCCGTTGTTCCCCGAGGCGCGGCGGCTCGCCGAGTGGCAGGCCGACTTCGCCGAACCGGTCTACGAGTTGCGCTGCATGGCGCTCTGCGACCGCTTGCGGTTGATGTATTTCGGCAACCTGTGGCAGGACTGGTCGGAGTTCGTCCTTGCCGACCTGGGTATCTATCGTTACGAAAGCGTCGAGTTTTCCGCCGACTCTCGCGGCTTTCGCCTGCGCGCCGACGTCGACGCCTATCTCCACCTGTTCGACTGCCGCCAGCGCTTCGACCTCGGCGAGCCGCTGGAGGAACTGCTCGCCGGCCTGCCGGGCGAACCCTACGCCAATCCCTGGCTGGAAGGGCGGCGGGTCAAGCTGCTGTTCCAGTTCGCCCAGCACTGCGAAAAGCAGCGGGATTTCGACCTGGCCCAGCGGCTCTATCGCCAATCCAGCCACCCCGGCGCGCGCCTGCGGGCGATTCGCAGCCTGGAACGCGGCGAGCGCTTCGCCGAAGCCCATGCATTGGCGCGGGAGGCCAGCTGTGCGCCGGAAAGCGACGCCGAACGCCAGGGCCTGGCCCGATTGCTGCCGCGCCTGCAAGGCAAGCTCGGCCTGCCGCGACAGGCGCGGGCGGCGGCTCCGGAAATCGACCGCCTGGACCTGTGCCTGGCGTTCCCGAGCGAACCCTGTAGCGTCGAGTGGGCGGTCCGCGAGCACCTGGAAGAGCCCGGTTGCGCGGTGCACTACGTCGAGAACGGCCTGATCAATTCGCTGTTCGGGCTGCTCTGCTGGGAGGCGATCTTCGCCGCCATTCCCGGGGCGTTCTTCCATCCCTTCCACAGCGCGCCGGCGGACCTGCACAGCGCCGACTTCCGCCAGCGCCGCGCCGCCCTGTTCGAGGCCTGCCTGGGACGGCTGGAGGACGGCAGCTACCGCGACGCGATCCGCTGTCGCTACCGCGACAAGTTCGGCCTGCAGTCGCCCTTCGTCTACTGGGAACTGCTTGGCGAGGAACTGCTCGAACAGGCGCTCGATTGCCTGCCGGCGGCCCACCTGCGCGCCTGGTTCGAACGCCTGCTGGAGGATATCCCGGGCAACCGCGCCGGCCTGCCGGACCTGATCCAGTTCTGGCCGGCGCAGCGGCGCTACCGGATGGTCGAGGTCAAGGGACCGGGCGACCGCCTGCAGGACAACCAGTTGCGCTGGCTGCAATTCTGCCGCGAGCGGGAGATGCCGGTGGCGGTCTGCTACGTGCGCTGGCATGTCGATGACTGAGCGGCCGC

Amino Acid Sequence

MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEIDVRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTFDRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEAVILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGEEVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLWDDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGWELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKELLEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAARGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEATSGSGHHHHHHSAGKETAAAKFERQHMDSPPPTGLVPRGSAGSGTIDDDDKSPGARGSMHEQYQAPLPVNSPALPEPFYYLHNFRAVLAWIGERYADLLDDQERAFIAAFAELPEASQALLVRMVMRKGTLFREGKLAYAEIGDTRAAVQPLLALGWVDAQPTLELAQLFGLLKKDELSQLFRDHLGRANLRKDALLERLQPLFPEARRLAEWQADFAEPVYELRCMALCDRLRLMYFGNLWQDWSEFVLADLGIYRYESVEFSADSRGFRLRADVDAYLHLFDCRQRFDLGEPLEELLAGLPGEPYANPWLEGRRVKLLFQFAQHCEKQRDFDLAQRLYRQSSHPGARLRAIRSLERGERFAEAHALAREASCAPESDAERQGLARLLPRLQGKLGLPRQARAAAPEIDRLDLCLAFPSEPCSVEWAVREHLEEPGCAVHYVENGLINSLFGLLCWEAIFAAIPGAFFHPFHSAPADLHSADFRQRRAALFEACLGRLEDGSYRDAIRCRYRDKFGLQSPFVYWELLGEELLEQALDCLPAAHLRAWFERLLEDIPGNRAGLPDLIQFWPAQRRYRMVEVKGPGDRLQDNQLRWLQFCREREMPVAVCYVRWHVDD*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU33180
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression with N-ter NUS tag to aid solubility; ; Estimated MW of expressed protein : 125286.21 Daltons; ; All MTMR15 (FAN1) plasmids MUST be grown at 30 degrees C or less to prevent recombination.