cDNA Clone FAN1 (C.elegans) (C01G5.8)

• Plasmid: pMal.His.FAN1 C.elegans
• Expressed: MBP-FAN1 C.elegans
• Genbank: NM_068596.3
• Tag: MBP
• Species: Caenorhabditis elegans

Sequence of Insert

GGATCCATGAAGAAAGCCAAGAAAGAGAAGAAAGAAAAGATCATTAAGCCAGACGGACCCATTAACTTTAATCGGAGTATCATGGCCGCTTTCGAGAAACAGAGCCGGGGCAAGATTTGCCCTCTGTGCGAAACTAAATTTTCACTGGCCAGCTACAAGTCCCACATGAACACCTGCAATGTGGCTGACGATGACGAGGAAATCGAAGTGATTGCCACATATACTCGCGACGAGGCTATCCTGATGAGGGCTGGACCAGAAATCATTCTGGGGGATGCCTCCTTCTCTGACAAGAGCGAGAACCCCACCAAACGGAGAAAGACAGATGAGCGGGAAGTGCCATCAGAGGATGACATTGTGCCCGAAGTGCCCGGACCTAGCGGCATCGTGAAGAACCACGAGATGCCTAGTGAATCACTGGACGTGACCGAGATCAGCGAAAATATTGAGAAAGTGATCAAGAAGAGCCCAGAGTGGATCAACCATCGGCGGCGGAGCAGCCGGCTGCTCCAGAACAGCCAGAAAGACCAGGCCGATAACGCTAATAAGGAGGACCCAGTGAAGAAAGAAACCGCCACAATTTCTGAGGTGCTCCAGGCTATCGAACGCTTCGAGCAGCGGGTGAGTGGACCAGAGCAGACATGGCCTTACTACATCAAAATCACTATCAAAATCATGAAGAGAGTGATCAGCACTGAGAAGTTCGACGGGACCTTTTACGCCGATGACTTTTGGCTGCCCTCCGATATCATTACATTCTATCGCTTTGTGGAGCTGCTGTCTGAAGGAGCTAAATGTCTGCTGGTGAGACTGTTCATCAGGAAGCCTGCCTGGTACAACCTGGAAAAACTGGAGCAGAAGTATCCTGAGATCCCAAATATCAAGGAGGCCGTGTCCGAACTGGCTAAGGGACACTTCATCGATGACGATTCTAGTATGAAAACCCTGGATGAGGCCCTCCAGATCAGCGACGTGGTGGCTCTGAAGAACGTGACCAAGAAATTCAAACTGGATGGCACAAAGAATCGCCAGGAGCTGATCCAGTCCCTGCGGAAATTTGCCCAGTCTCAGCAGAGTATTTTCGGCGGGACCGGCAACGTGGAGAAGAGCATCCTGAAATCCCTGAAGCAGGAACTGGGACCATGCGTGAGAGTGAGGGGAGGATTCGTGGACCTGTTTAAGTGCCTGTTCACAATCTACTGTCCCGTGACCACAAACTCAGCCAATGTGATCGACAACCCTAGCACTACCAACGTGTACCAGGATCTGCTGTATCTGATGCTGTCCGTGGCCAACGGGACAGTGCAGTTTCCAGCTCCCAATCCTTGTCCAATCATTGCCTCTTTCTACAAAAACAGGAATATGCTCCAGGATTATATGATCAGCAAGTCCCTGGAGATCGCCATCGTGAGCCAGATGAGCAACGGAAATCTGGACGCCGCTCTGGATCTGGCCATTGACGCTAAAGAGTTCATCGAGCAGATGAGCGACGATGACAAGAGATACTATGAATCCCTGGAGATTCACGAAAGGAAGTTTACAAGCATCTGGGTGTTCACTCGCTGCTGTGGGCATGCTTCAAGCATCCTGGAGCGGCAGAAGAAATACGGAATGGCCGTGGAATGGCAGAAGGACCTGCTGATTACCAACAAGGACATCCAGTCTTATTGCATTGATAGTCGCGGCATCTGGTGGGACCGGATGCTGCTGAATCTGGATAGTCACCTGAAAGAGAAGAAAGAATGTGCCAAGATGATCCAGATCGCCCTCCAGGACCCCTCTATTCTGGAGAAGGAACTGCTGATGATCCAGGACAGGGCTCTGAAGCTGAAAGAGATGCCAGCCGATTTCGTGACACCCATCAACATTGGGAATCCTGAAAAGAAAACTATTACCGCCAACGTGATCACTAAGAGCCTGGGGGACGGACGCATTAATCGGTTTATGATCCGCGATCACGAAACTGATGACGATGTGGAGTGCAGCGTGGAGGAAGTGACCAGAAGGCATTACCTGGAGAACGAAGGGTTCTCCACAGGAGTGCACGATGAGGGATCTACATGGCATACTCTGTTTGGCCTGTTCTTTTATGATGTGATCTTCGCCACTGACGAGTCCGTGGAATCTACCTGGCTGTCAGAGCTGCAAGATTGCCCAAGTGACCTGTCAAACACTCTGTACAGCAAGAGAAAAGAGAAGTTTGAAGACAGGTTCGTGTGGCTGGAGGAAGCTGAGCAGGAACTGATCGAGGAAAACATCAGGAAAATTTGGGACCTGAAGCACAACGAGACCAATCGCGAATGTAGTTGGAAGCAGTTCCCTATGGGCGCCGAGGATTGCGTGTCATTCTTTCAGTGTATCCCCCGGCCTGCTCTGATCCTGATTCTGCGCCGGCTGGCCGAGAACTATAGAAATTCTAGGAGTGGGTTTCCTGACCTGACCCTGTGGAACCCAGAAACAAAAAGAGTGGCTGTGGTGGAGGTGAAGGGACCAGGGGACAGACTGAGCACCAAGCAGAGGCTGTGGCTGGCCATTTTCGCTGATTCCGGCATCCGGGCTGAGGTGTGCCATGTGGCTGCTCAGAACAGCCGGCTGCTGGTGTGAGCGGCCGC

Amino Acid Sequence

MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQTNSSSNNNNNNNNNNLGLEVLFQGPLGSMKKAKKEKKEKIIKPDGPINFNRSIMAAFEKQSRGKICPLCETKFSLASYKSHMNTCNVADDDEEIEVIATYTRDEAILMRAGPEIILGDASFSDKSENPTKRRKTDEREVPSEDDIVPEVPGPSGIVKNHEMPSESLDVTEISENIEKVIKKSPEWINHRRRSSRLLQNSQKDQADNANKEDPVKKETATISEVLQAIERFEQRVSGPEQTWPYYIKITIKIMKRVISTEKFDGTFYADDFWLPSDIITFYRFVELLSEGAKCLLVRLFIRKPAWYNLEKLEQKYPEIPNIKEAVSELAKGHFIDDDSSMKTLDEALQISDVVALKNVTKKFKLDGTKNRQELIQSLRKFAQSQQSIFGGTGNVEKSILKSLKQELGPCVRVRGGFVDLFKCLFTIYCPVTTNSANVIDNPSTTNVYQDLLYLMLSVANGTVQFPAPNPCPIIASFYKNRNMLQDYMISKSLEIAIVSQMSNGNLDAALDLAIDAKEFIEQMSDDDKRYYESLEIHERKFTSIWVFTRCCGHASSILERQKKYGMAVEWQKDLLITNKDIQSYCIDSRGIWWDRMLLNLDSHLKEKKECAKMIQIALQDPSILEKELLMIQDRALKLKEMPADFVTPINIGNPEKKTITANVITKSLGDGRINRFMIRDHETDDDVECSVEEVTRRHYLENEGFSTGVHDEGSTWHTLFGLFFYDVIFATDESVESTWLSELQDCPSDLSNTLYSKRKEKFEDRFVWLEEAEQELIEENIRKIWDLKHNETNRECSWKQFPMGAEDCVSFFQCIPRPALILILRRLAENYRNSRSGFPDLTLWNPETKRVAVVEVKGPGDRLSTKQRLWLAIFADSGIRAEVCHVAAQNSRLLV*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU19998
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express Protein with N-ter MBP.; ; Estimated MW of expressed protein : 145105.75 Daltons; ; All MTMR15 (FAN1) plasmids MUST be grown at 30 degrees C or less to prevent recombination.