cDNA Clone NEDD4

• Synonyms: PIG53, NEDD4-1, RPF1
• Plasmid: pcDNA5 FRT/TO GFP NEDD4
• Parent Plasmid: pcDNA5 FRT/TO GFP
• Expressed: GFP-NEDD4
• Genbank: NM_006154.2
• Tag: GFP
• Species: Human

Sequence of Insert

GGATCCATGGCAACTTGCGCGGTGGAGGTGTTCGGGCTCCTGGAGGACGA GGAAAATTCACGAATTGTGAGAGTAAGAGTTATAGCCGGAATAGGCCTTG CCAAGAAGGATATATTGGGAGCTAGTGATCCTTACGTGAGAGTGACGTTA TATGACCCAATGAATGGAGTTCTTACAAGTGTGCAAACAAAAACCATTAA AAAGAGTTTGAATCCAAAGTGGAATGAAGAAATATTATTCAGAGTTCATC CTCAGCAGCACCGGCTTCTTTTTGAAGTGTTTGACGAAAACCGATTGACA AGAGATGATTTCCTAGGTCAAGTGGATGTTCCACTTTATCCATTACCGAC AGAAAATCCAAGATTGGAGAGACCATATACATTTAAGGATTTTGTTCTTC ATCCAAGAAGTCACAAATCAAGAGTTAAAGGTTATCTGAGACTAAAAATG ACTTATTTACCTAAAACCAGTGGCTCAGAAGATGATAATGCAGAACAGGC TGAGGAATTAGAGCCTGGCTGGGTTGTTTTGGACCAACCAGATGCTGCTT GCCATTTGCAGCAACAACAAGAACCTTCTCCTCTACCTCCAGGGTGGGAA GAGAGGCAGGATATCCTTGGAAGGACCTATTATGTAAACCATGAATCTAG AAGAACACAGTGGAAAAGACCAACCCCTCAGGACAACCTAACAGATGCTG AGAATGGCAACATTCAACTGCAAGCACAACGTGCATTTACCACCAGGCGG CAGATATCCGAGGAAACAGAAAGTGTTGACAACCAAGAGTCTTCCGAGAA CTGGGAAATTATAAGAGAAGATGAAGCCACCATGTATAGCAGCCAGGCCT TCCCATCACCTCCACCGTCAAGTAACTTGGATGTTCCAACTCATCTTGCA GAAGAATTGAATGCCAGACTCACCATTTTTGGAAATTCAGCCGTGAGCCA GCCAGCATCGAGCTCAAATCATTCCAGCAGAAGAGGCAGCTTACAAGCCT ATACTTTTGAGGAACAACCTACACTTCCTGTGCTTTTGCCTACTTCATCT GGATTACCACCAGGTTGGGAAGAAAAACAAGATGAAAGAGGAAGATCATA TTATGTAGATCACAATTCCAGAACGACTACTTGGACAAAGCCCACTGTAC AGGCCACAGTGGAGACCAGTCAGCTGACCTCAAGCCAGAGTTCTGCAGGC CCTCAATCACAAGCCTCCACCAGTGATTCAGGCCAGCAGGTGACCCAGCC ATCTGAAATTGAGCAAGGATTCCTTCCTAAAGGCTGGGAAGTCCGGCATG CACCAAATGGGAGGCCTTTCTTTATTGACCACAACACTAAAACCACCACC TGGGAAGATCCAAGATTGAAAATTCCAGCCCATCTGAGAGGAAAGACATC ACTTGATACTTCCAATGATCTAGGGCCTTTACCTCCAGGATGGGAAGAGA GAACTCACACAGATGGAAGAATCTTCTACATAAATCACAATATAAAAAGA ACACAATGGGAAGATCCTCGGTTGGAGAATGTAGCAATAACTGGACCAGC AGTGCCCTACTCCAGGGATTACAAAAGAAAGTATGAGTTCTTCCGAAGAA AGTTGAAGAAGCAGAATGACATTCCAAACAAATTTGAAATGAAACTTCGC CGAGCAACTGTTCTTGAAGACTCTTACCGGAGAATTATGGGTGTCAAGAG AGCAGACTTCCTGAAGGCTCGACTGTGGATTGAGTTTGATGGTGAAAAGG GATTGGATTATGGAGGAGTTGCCAGAGAATGGTTCTTCCTGATCTCAAAG GAAATGTTTAACCCTTATTATGGGTTGTTTGAATATTCTGCTACGGACAA TTATACCCTACAGATAAATCCAAACTCTGGATTGTGTAACGAAGATCACC TCTCTTACTTCAAGTTTATTGGTCGGGTAGCTGGAATGGCAGTTTATCAT GGCAAACTGTTGGATGGTTTTTTCATCCGCCCATTTTACAAGATGATGCT TCACAAACCAATAACCCTTCATGATATGGAATCTGTGGATAGTGAATATT ACAATTCCCTAAGATGGATTCTTGAAAATGACCCAACAGAATTGGACCTC AGGTTTATCATAGATGAAGAACTTTTTGGACAGACACATCAACATGAGCT GAAAAATGGTGGATCAGAAATAGTTGTCACCAATAAGAACAAAAAGGAAT ATATTTATCTTGTAATACAATGGCGATTTGTAAACCGAATCCAGAAGCAA ATGGCTGCTTTTAAAGAGGGATTCTTTGAACTAATACCACAGGATCTCAT CAAAATTTTTGATGAAAATGAACTAGAGCTTCTTATGTGTGGACTGGGAG ATGTTGATGTGAATGACTGGAGGGAACATACAAAGTATAAAAATGGCTAC AGTGCAAATCATCAGGTTATACAGTGGTTTTGGAAGGCTGTTTTAATGAT GGATTCAGAAAAAAGAATAAGATTACTTCAGTTTGTCACTGGCACATCTC GGGTGCCTATGAATGGATTTGCTGAACTATACGGTTCAAATGGACCACAG TCATTTACAGTTGAACAGTGGGGTACGCCTGAAAAGCTGCCAAGAGCTCA TACCTGTTTTAATCGCCTGGACTTGCCACCTTATGAATCATTTGAAGAAT TATGGGATAAACTTCAGATGGCAATTGAAAACACCCAGGGCTTTGATGGA GTTGATTAGGCGGCCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSMATCAV
EVFGLLEDEENSRIVRVRVIAGIGLAKKDILGASDPYVRVTLYDPMNGVL
TSVQTKTIKKSLNPKWNEEILFRVHPQQHRLLFEVFDENRLTRDDFLGQV
DVPLYPLPTENPRLERPYTFKDFVLHPRSHKSRVKGYLRLKMTYLPKTSG
SEDDNAEQAEELEPGWVVLDQPDAACHLQQQQEPSPLPPGWEERQDILGR
TYYVNHESRRTQWKRPTPQDNLTDAENGNIQLQAQRAFTTRRQISEETES
VDNQESSENWEIIREDEATMYSSQAFPSPPPSSNLDVPTHLAEELNARLT
IFGNSAVSQPASSSNHSSRRGSLQAYTFEEQPTLPVLLPTSSGLPPGWEE
KQDERGRSYYVDHNSRTTTWTKPTVQATVETSQLTSSQSSAGPQSQASTS
DSGQQVTQPSEIEQGFLPKGWEVRHAPNGRPFFIDHNTKTTTWEDPRLKI
PAHLRGKTSLDTSNDLGPLPPGWEERTHTDGRIFYINHNIKRTQWEDPRL
ENVAITGPAVPYSRDYKRKYEFFRRKLKKQNDIPNKFEMKLRRATVLEDS
YRRIMGVKRADFLKARLWIEFDGEKGLDYGGVAREWFFLISKEMFNPYYG
LFEYSATDNYTLQINPNSGLCNEDHLSYFKFIGRVAGMAVYHGKLLDGFF
IRPFYKMMLHKPITLHDMESVDSEYYNSLRWILENDPTELDLRFIIDEEL
FGQTHQHELKNGGSEIVVTNKNKKEYIYLVIQWRFVNRIQKQMAAFKEGF
FELIPQDLIKIFDENELELLMCGLGDVDVNDWREHTKYKNGYSANHQVIQ
WFWKAVLMMDSEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQSFTVEQWG
TPEKLPRAHTCFNRLDLPPYESFEELWDKLQMAIENTQGFDGVD*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU19742
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee