cDNA Clone TRIM65

• Plasmid: pCMV5-HA TRIM65 C35/A
• Parent Plasmid: pCMV5 HA1
• Expressed: HA-TRIM65 C35/A
• Genbank: NM_173547.2
• Tag: HA
• Species: Human

Sequence of Insert

AGATCTATGGCCGCGCAGCTGCTGGAGGAGAAGCTGACCTGCGCCATCTGCCTGGGGCTCTACCAGGACCCAGTGACGCTGCCCTGCGGCCACAACTTCTGCGGGGCCGCCATCCGGGACTGGTGGGACCGCTGCGGAAAGGCGTGCCCCGAGTGCCGGGAGCCCTTTCCCGACGGCGCCGAGCTGCGCCGCAACGTGGCCCTCAGCGGCGTGCTGGAGGTGGTGCGCGCCGGGCCCGCCCGGGATCCCGGCCCCGATCCCGGCCCCGGCCCCGACCCTGCCGCGCGCTGCCCCCGCCACGGGCGGCCGCTGGAGCTCTTCTGCCGGACCGAGGGCCGCTGTGTGTGCAGCGTGTGCACCGTGCGCGAGTGTCGCCTCCACGAGCGGGCGCTGCTGGATGCCGAGCGCCTCAAGCGCGAGGCCCAGCTGAGAGCCAGCCTGGAGGTTACCCAGCAGCAGGCCACCCAGGCCGAAGGCCAGCTACTAGAGCTGCGCAAGCAAAGCAGCCAGATCCAGAACTCGGCCTGCATCTTGGCCTCCTGGGTCTCCGGCAAGTTCAGCAGCCTGCTACAGGCCCTGGAAATACAGCACACGACAGCACTGAGGAGCATCGAGGTGGCCAAGACGCAGGCGCTGGCACAGGCTCGAGACGAGGAGC-AGCGGCTGCGGGG-CCACTTGGAGGCTGTGGCTCGCCATGGCTGCAGGATCCGGGAGCTCCTGGAGCAGGTGGATGAGCAGACCTTTCTGCAGGAATCGCAGCTCCTCCAGCCCCCAGGGCCTCTTGGGCCACTGACCCCTCTGCAGTGGGATGAAGACCAACAGCTGGGTGACCTGAAGCAGTTGCTAAGCCGGCTGTGTGGCCTCCTCTTGGAAGAGGGGAGCCACCCTGGGGCACCAGCCAAGCCTGTGGACTTAGCCCCCGTGGAGGCCCCAGGTCCCCTGGCACCGGTCCCAAGCACAGTTTGTCCACTGAGGAGGAAACTCTGGCAGAATTATCGCAATCTGACCTTTGATCCAGTCAGCGCCAACCGTCACTTCTATCTGTCGCGCCAGGACCAGCAGGTGAAGCACTGTCGTCAGTCCCGGGGCCCAGGCGGGCCCGGCAGCTTTGAGCTCTGGCAGGTGCAATGTGCCCAGAGCTTCCAGGCCGGGCACCACTACTGGGAGGTGCGCGCGTCAGACCACTCGGTGACACTGGGCGTCTCCTACCCGCAACTGCCACGGTGCAGGCTGGGGCCCCACACAGACAACATTGGCCGGGGACCCTGCTCCTGGGGGCTCTGCGTCCAGGAGGACAGCCTCCAGGCCTGGCACAACGGGGAAGCCCAGCGCCTCCCAGGGGTGTCAGGGCGGCTCCTGGGCATGGATTTGGACCTGGCCTCAGGCTGCCTCACCTTCTACAGCCTGGAGCCCCAGACCCAGCCCCTGTACACCTTCCATGCCCTCTTCAACCAGCCCCTCACCCCCGTCTTCTGGCTCCTCGAGGGTAGGACCCTGACCCTGTGCCATCAGCCAGGGGCTGTGTTCCCTCCGGGGCCCCAGGAAGAGGTGCTCAGCTGAAGATCT

Amino Acid Sequence

MYPYDVPDYAGSMAAQLLEEKLTCAICLGLYQDPVTLPCGHNFCGAAIRDWWDRCGKACPECREPFPDGAELRRNVALSGVLEVVRAGPARDPGPDPGPGPDPAARCPRHGRPLELFCRTEGRCVCSVCTVRECRLHERALLDAERLKREAQLRASLEVTQQQATQAEGQLLELRKQSSQIQNSACILASWVSGKFSSLLQALEIQHTTALRSIEVAKTQALAQARDEEQRLRGHLEAVARHGCRIRELLEQVDEQTFLQESQLLQPPGPLGPLTPLQWDEDQQLGDLKQLLSRLCGLLLEEGSHPGAPAKPVDLAPVEAPGPLAPVPSTVCPLRRKLWQNYRNLTFDPVSANRHFYLSRQDQQVKHCRQSRGPGGPGSFELWQVQCAQSFQAGHHYWEVRASDHSVTLGVSYPQLPRCRLGPHTDNIGRGPCSWGLCVQEDSLQAWHNGEAQRLPGVSGRLLGMDLDLASGCLTFYSLEPQTQPLYTFHALFNQPLTPVFWLLEGRTLTLCHQPGAVFPPGPQEEVLS*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU19723
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: C35/A mutant. Two known variations V222G and L509P (benign according to ensembl). Two silent changes T>C 669 bp and C>T 738 bp.