cDNA Clone RCOR1

• Synonyms: RCOR; COREST
• Plasmid: pCMV5-HA RCOR1 4-end delta A27
• Parent Plasmid: pCMV5 HA1
• Expressed: HA-RCOR1 4-end delta A27
• Genbank: NM_015156.4
• Tag: HA
• Species: Human

Sequence of Insert

GGATCCATGGTGGAGAAGGGCCCCGAGGTCTCAGGGAAGCGGAGAGGGAGGAACAACGCGGCCGCCTCCGCCTCCGCCGCCGCCTCCGCCGCCGCCTCGGCCGCCTGCGCCTCGCCAGCCGCCACTGCCGCCTCGGGCGCCGCCGCCTCCTCAGCCTCGGCCGCCGCCGCCTCAGCCGCCGCCGCCCCCAATAATGGCCAGAATAAAAGTTTGGCGGCGGCGGCGCCCAATGGCAACAGCAGCAGCAACTCCTGGGAGGAAGGCAGCTCGGGCTCGTCCAGCGACGAGGAGCACGGTGGCGGTGGCATGAGGGTCGGACCCCAGTACCAGGCGGTGGTGCCCGACTTCGACCCCGCCAAACTGGCAAGACGCAGTCAAGAACGGGACAATCTTGGCATGTTGGTCTGGTCACCCAATCAAAATCTGTCAGAAGCAAAGTTGGATGAATACATTGCCATTGCCAAAGAAAAGCATGGGTACAACATGGAACAGGCTCTTGGGATGCTCTTCTGGCATAAACATAATATCGAAAAGTCATTGGCTGATTTGCCCAACTTTACCCCTTTCCCAGATGAGTGGACTGTGGAAGATAAAGTCTTATTTGAGCAAGCCTTTAGTTTTCATGGGAAAACTTTTCATAGAATCCAACAAATGCTTCCAGATAAATCTATAGCAAGTCTGGTGAAATTTTACTATTCTTGGAAGAAGACGAGGACTAAAACTAGTGTGATGGATCGCCATGCCCGGAAACAAAAACGGGAGCGGGAGGAGAGCGAGGATGAACTGGAAGAGGCAAATGGAAACAATCCCATTGACATTGAGGTTGATCAAAACAAGGAAAGCAAAAAGGAGGTTCCCCCTACTGAGACAGTTCCTCAGGTCAAAAAAGAAAAACATAGCACACAAGCTAAAAATAGAGCAAAAAGGAAACCTCCAAAAGGAATGTTTCTTTCTCAAGAAGATGTGGAGGCTGTTTCTGCCAATGCCACTGCTGCTACCACGGTGCTGAGACAACTAGACATGGAATTGGTTTCAGTCAAACGACAGATCCAGAATATTAAACAGACAAACAGTGCTCTCAAAGAAAAACTTGATGGTGGAATAGAACCATATCGACTTCCAGAGGTCATTCAGAAATGTAATGCACGTTGGACTACAGAAGAGCAGCTTCTCGCCGTACAAGCCATCAGGAAATATGGCCGAGATTTTCAGGCAATCTCAGACGTGATTGGGAACAAATCAGTGGTACAAGTGAAAAACTTTTTTGTAAATTATCGACGCCGCTTCAACATAGATGAAGTTTTACAAGAATGGGAGGCAGAACATGGTAAAGAAGAGACCAATGGGCCCAGTAACCAGAAGCCTGTGAAGTCCCCAGATAATTCCATTAAGATGCCCGAAGAGGAAGACGAGGCTCCTGTTCTGGATGTCAGATATGCATCTGCCTCCTGAGGATCC

Amino Acid Sequence

MYPYDVPDYAGSMVEKGPEVSGKRRGRNNAAASASAAASAAASAACASPAATAASGAAASSASAAAASAAAAPNNGQNKSLAAAAPNGNSSSNSWEEGSS
GSSSDEEHGGGGMRVGPQYQAVVPDFDPAKLARRSQERDNLGMLVWSPNQNLSEAKLDEYIAIAKEKHGYNMEQALGMLFWHKHNIEKSLADLPNFTPFP
DEWTVEDKVLFEQAFSFHGKTFHRIQQMLPDKSIASLVKFYYSWKKTRTKTSVMDRHARKQKREREESEDELEEANGNNPIDIEVDQNKESKKEVPPTET
VPQVKKEKHSTQAKNRAKRKPPKGMFLSQEDVEAVSANATAATTVLRQLDMELVSVKRQIQNIKQTNSALKEKLDGGIEPYRLPEVIQKCNARWTTEEQL
LAVQAIRKYGRDFQAISDVIGNKSVVQVKNFFVNYRRRFNIDEVLQEWEAEHGKEETNGPSNQKPVKSPDNSIKMPEEEDEAPVLDVRYASAS*

• Vector Type: Mammalian
• Antibiotic: Amp
• Full Snapgene Map: pCMV5-HA RCOR1 4-end delta A2755.6 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU19711
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Missing first 3 residues and A27 as compared to reference sequence. Clone is difficult to sequence, use GC melt if possible. May be prone to rearrangement due to repetitive nature, growth at 30oC recommended.
• Low Temp: No