cDNA Clone CDC45 (yeast)

• Plasmid: pRS416 PRO CDC45 y 3′UTR K576/T 3′NheI
• Parent Plasmid: pRS416
• Genbank: U56821.1 GI:1572488
• Species: Yeast

Sequence of Insert

GGATCCCATCACAAGGGTTATTTAAAACTTGATCTTCATATGATTGTTGC
TGTTGCTGTTGCTGTTGCTGATGATGACCGAAATTGAAGAAGGCATTCAC
TCCAGTGCTGCCAAAAATAAACAATGCAAAGATGAGAACGATACTCCAGC
GACTTTGACTCATACTACAGTAAAATTTGGTCGTTTTTTGCCTGGCAAAC
CATGCCTATTTTTCGTCATTAAAGGTCTTCTGAGCTTTCATCAAAATTAT
CTCTATAATTATAAAGGACAGCTCAGAGCCACACCCAAACATCTGCAAAG
GTAAAGGCCATCTTTCATCTCACATTGCCTAACAAGAAAGTCACATTGCA
ATTGTTTCGAGAAGAAAACATTAATTCAATTACGTGGTTCCCATGCGTCT
GTGTTAAGCGTCAATTTATCATTTGACGAACTCGTTGCAACCACAAACAA
AGCAAGGGCCGCATTTATCAAAGGAAAACCAACCAAAAGCATAAATAAAC
GAGATTTTTATTATGAAAAAGCCATGCGAATCCTACTAGAATAAATTCAA
AGTGAAGACATTTAGTCAATTCGATCTTCTCTCTCTTGCTTTAACTAGTC
GTAAAACCTAATAAGTTTCATTCACGACGCGTATTTTTTTTTCAACATAT
CATCTAACGCGTTTTAAGAAATTTCAGTAAATAATGAAGCTGTAGTAATG
CCAACAATCCATTGTGGCTACTTTATACAACGCACTATAGAGCAATAAAA
GTGTAGCAATAAACACTAGAGAGAAGGCACATAATAACAAGAATATACTC
TCGCACATGTATTATGGAATCAGCCAGTTTAGCGAAGCCTACAACAAAAT
CTTAAGGAATTCATCGTCTCATTCATCATGTCAATTGGTCATTTTCGTTT
CTTGTCTTAACATCGATGCGCTGTGTGCGACGAAGATGTTGTCACTATTA
TTCAAAAAACAATTGGTACAATCTCAAATAGTACCGATATTTGGGTACTC
TGAATTACGACGCCACTATTCTCAATTGGATGACAATATAAATAGTCTGC
TATTAGTAGGGTTTGGAGGCGTTATTGATCTGGAGGCTTTCTTAGAAATC
GATCCCCAAGAGTATGTTATCGATACAGATGAAAAGTCGGGGGAACAAAG
TTTCAGGAGAGACATCTACGTGTTGGATGCTCATAGACCGTGGAATCTCG
ACAATATATTTGGATCACAAATCATCCAATGTTTTGACGACGGTACGGTG
GATGACACATTAGGCGAACAAAAAGAAGCATACTATAAATTGCTTGAACT
GGACGAGGAGAGTGGTGACGATGAGCTCTCAGGTGACGAGAACGATAATA
ATGGGGGTGACGATGAGGCAACTGATGCCGATGAAGTCACAGACGAGGAC
GAAGAAGACGAAGATGAGACAATATCTAATAAAAGAGGTAATTCTTCAAT
AGGACCGAATGATCTCTCAAAAAGAAAGCAACGAAAGAAGCAAATCCATG
AATATGAAGGTGTTTTGGAGGAATACTATTCTCAAGGCACAACAGTGGTT
AACTCGATATCTGCACAAATCTATTCATTATTGTCTGCTATCGGAGAAAC
AAATCTTTCAAACCTGTGGTTGAATATACTTGGTACGACCTCGTTAGATA
TAGCATATGCTCAAGTTTACAATCGATTATACCCCTTATTGCAAGATGAA
GTGAAGCGTCTCACACCAAGCAGTAGAAACTCAGTAAAGACCCCTGATAC
ATTGACGTTAAACATCCAACCGGATTACTACCTTTTCTTACTGAGGCATT
CTTCATTGTACGACAGTTTTTATTATTCTAACTATGTCAATGCCAAACTA
TCCCTATGGAATGAAAATGGGAAAAAGAGATTGCATAAGATGTTTGCTAG
AATGGGTATACCATTAAGTACTGCACAAGAAACATGGTTATACATGGATC
ATTCTATTAAGAGAGAACTTGGGATAATATTTGACAAAAATTTGGATCGT
TATGGGTTGCAAGATATAATTAGAGATGGGTTTGTTAGAACTCTTGGATA
TCGTGGGTCCATAAGTGCCAGTGAATTTGTCGAAGCACTAACAGCTCTTT
TGGAAGTAGGTAATTCAACTGATAAGGATAGTGTGAAAATAAATAATGAC
AATAATGACGATACAGATGGAGAGGAAGAAGAAGATAACAGCGCTCAAAA
ATTGACGAATTTGAGAAAAAGATGGGTTTCGAATTTTTGGCTCAGTTGGG
ATGCTCTAGATGACAGAAAGGTGGAACTATTAAACCGTGGCATTCAACTA
GCACAAGACTTACAGAGAGCAATTTTTAATACCGGGGTTGCTATATTGGA
GAAGAAATTAATCAAGCATTTAAGAATTTATAGATTATGCGTCTTACAAG
ACGGACCCGATTTAGACTTGTACAGAAACCCATTGACGTTATTAAGATTA
GGAAATTGGCTCATAGAATGCTGTGCGGAATCTGAAGACAAGCAATTGTT
GCCCATGGTGCTTGCCAGCATAGATGAAAATACGGACACTTACTTGGTTG
CTGGGTTAACACCTAGGTATCCTCGCGGACTAGACACGATACACACAAAA
AAACCTATTTTGAATAATTTCAGCATGGCGTTTCAACAAATAACTGCAGA
AACGGATGCTAAAGTGAGAATAGATAATTTTGAAAGTTCCATAATTGAAA
TACGTCGTGAAGATCTTTCACCATTCCTGGAGAAGCTGACCTTGAGTGGA
TTGTTAGCTAGCTAATCAAATTTATATTATTTAGTCGTACATATATACCA
GCATATGAATATAAAATCTAAGCTTTTTCAAGCTTTTAAGTCCAACTTTT
CTATAATAATACCATATTTTGTGCGCGGCTCTTGACAAAACAAAAAAAAA
AACCTAAATAAAAGGAAATTAAGAACAGGGAACTAGGTTTGGAAGGACAA
ATTCAGGAAGGAAAGGCGGCCGC

Amino Acid Sequence

MYYGISQFSEAYNKILRNSSSHSSCQLVIFVSCLNIDALCATKMLSLLFK
KQLVQSQIVPIFGYSELRRHYSQLDDNINSLLLVGFGGVIDLEAFLEIDP
QEYVIDTDEKSGEQSFRRDIYVLDAHRPWNLDNIFGSQIIQCFDDGTVDD
TLGEQKEAYYKLLELDEESGDDELSGDENDNNGGDDEATDADEVTDEDEE
DEDETISNKRGNSSIGPNDLSKRKQRKKQIHEYEGVLEEYYSQGTTVVNS
ISAQIYSLLSAIGETNLSNLWLNILGTTSLDIAYAQVYNRLYPLLQDEVK
RLTPSSRNSVKTPDTLTLNIQPDYYLFLLRHSSLYDSFYYSNYVNAKLSL
WNENGKKRLHKMFARMGIPLSTAQETWLYMDHSIKRELGIIFDKNLDRYG
LQDIIRDGFVRTLGYRGSISASEFVEALTALLEVGNSTDKDSVKINNDNN
DDTDGEEEEDNSAQKLTNLRKRWVSNFWLSWDALDDRKVELLNRGIQLAQ
DLQRAIFNTGVAILEKKLIKHLRIYRLCVLQDGPDLDLYRNPLTLLRLGN
WLIECCAESEDKQLLPMVLASIDENTDTYLVAGLTPRYPRGLDTIHTKKP
ILNNFSMAFQQITAETDAKVRIDNFESSIIEIRREDLSPFLEKLTLSGLL
*

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU19652
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mutagenesis to introduce Nhe1 site at 3’ end of CDC45 ORF.