cDNA Clone NUCB1

• Synonyms: NUC; CALNUC
• Plasmid: pcDNA5 FRT/TO HA NUCB1 L251P
• Parent Plasmid: pcDNA5 FRT/TO HA2
• Expressed: HA NUCB1 L251P
• Genbank: NM_006184.6
• Tag: HA
• Species: Human

Sequence of Insert

GCGGCCGCGCCTCCCTCTGGGCCCCGAGGGACCCTCCTTCTGTTGCCGCTGCTGCTGCTGCTCCTGCTTCGCGCCGTGCTGGCTGTCCCCCTGGAGCGAGGGGCGCCCAACAAGGAGGAGACCCCTGCGACTGAGAGTCCCGACACAGGCCTGTACTACCACCGGTACCTCCAGGAGGTCATCGATGTACTGGAGACGGATGGGCATTTCCGAGAGAAGCTGCAGGCTGCCAATGCGGAGGACATCAAGAGCGGGAAGCTGAGCCGAGAGCTGGACTTTGTCAGCCACCACGTCCGCACCAAGCTGGATGAGCTCAAGCGACAGGAGGTGTCACGGCTGCGGATGCTGCTCAAGGCCAAGATGGACGCCGAGCAGGATCCCAATGTACAGGTGGATCATCTGAATCTCCTGAAACAGTTTGAACACCTGGACCCTCAGAACCAGCATACATTCGAGGCCCGCGACCTGGAGCTGCTGATCCAGACGGCCACCCGGGACCTTGCCCAGTACGACGCAGCCCATCATGAAGAGTTCAAGCGCTACGAGATGCTTAAGGAACACGAGAGACGGCGTTATCTGGAGTCACTGGGAGAGGAGCAGAGAAAGGAGGCGGAGAGGAAGCTGGAAGAGCAACAGCGCCGGCACCGCGAGCACCCTAAAGTCAACGTGCCTGGCAGCCAAGCCCAGTTGAAGGAGGTGTGGGAGGAGCTGGATGGACTGGACCCCAACAGGTTTAACCCCAAGACCTTCTTCATACCGCATGATATCAACAGTGATGGTGTCCTGGATGAGCAGGAGCTGGAGGCACTCTTCACCAAGGAGCTGGAGAAAGTGTACGACCCGAAGAATGAGGAGGACGACATGCGGGAGATGGAGGAGGAGCGACTGCGCATGCGGGAGCATGTGATGAAGAATGTGGACACCAACCAGGACCGCCTCGTGACCCTGGAGGAGTTCCTCGCATCCACTCAGAGGAAGGAGTTTGGGGACACCGGGGAGGGCTGGGAGACAGTGGAGATGCACCCTGCCTACACCGAGGAAGAGCTGAGGCGCTTTGAAGAGGAGCTGGCTGCCCGGGAGGCAGAGCTGAATGCCAAGGCCCAGCGCCTCAGCCAGGAGACAGAGGCTCTAGGGCGGTCCCAGGGCCGCCTGGAGGCCCAGAAGAGAGAGCTGCAGCAGGCTGTGCTGCACATGGAGCAGCGGAAGCAGCAGCAGCAGCAGCAGCAAGGCCACAAGGCCCCGGCTGCCCACCCTGAGGGGCAGCTCAAGTTCCACCCAGACACAGACGATGTACCTGTCCCAGCTCCAGCCGGTGACCAGAAGGAGGTGGACACTTCAGAAAAGAAACTTCTCGAGCGGCTCCCTGAGGTTGAGGTGCCCCAGCATCTGTGAGCGGCCGC

Amino Acid Sequence

MYPYDVPDYAGIPGIPGSTRAAAPPSGPRGTLLLLPLLLLLLLRAVLAVPLERGAPNKEETPATESPDTGLYYHRYLQEVIDVLETDGHFREKLQAANAE
DIKSGKLSRELDFVSHHVRTKLDELKRQEVSRLRMLLKAKMDAEQDPNVQVDHLNLLKQFEHLDPQNQHTFEARDLELLIQTATRDLAQYDAAHHEEFKR
YEMLKEHERRRYLESLGEEQRKEAERKLEEQQRRHREHPKVNVPGSQAQLKEVWEELDGLDPNRFNPKTFFIPHDINSDGVLDEQELEALFTKELEKVYD
PKNEEDDMREMEEERLRMREHVMKNVDTNQDRLVTLEEFLASTQRKEFGDTGEGWETVEMHPAYTEEELRRFEEELAAREAELNAKAQRLSQETEALGRS
QGRLEAQKRELQQAVLHMEQRKQQQQQQQGHKAPAAHPEGQLKFHPDTDDVPVPAPAGDQKEVDTSEKKLLERLPEVEVPQHL*

• Vector Type: Mammalian
• Antibiotic: Amp
• Full Snapgene Map: pcDNA5 FRT/TO HA NUCB1 L251P101.91 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU19593
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Compared to reference sequence this clone has a mutation at L251P and two silent changes at G8 and P279.
• Low Temp: No