cDNA Clone MCM3 (yeast)

• Plasmid: pRS415 MCM3 c-6H3M
• Parent Plasmid: pRS415
• Genbank: NP_10882
• Species: Yeast

Sequence of Insert

ACTAGTAACCGGGTTTTTTTCTTTGCAATTTTTTTTTCGTTCTCCTAAAGCATACACAAATAAATCCTTTTTTTTATTTTCTATTTATTTTGTTATTTATCATCTATATAGCAATAATATACTTTGTTTTTATTCGTATTTCACACTTTTCTTTTTCCTTATGCAGGCAGTGTAATTCATTGGGGAGGATGATTTTCATGTGCGCATATCTACCGGCTGCAAGCAGCCGGTCGGTGGCAAATCCGGCGCTTCCCCCTCAAAAAAAAAAAAAAAAAAAAAAAAAGGGAACTCTCAGAACGGGGGAGGTTGAAGAGCAGGCCAAGGGAAATATTAGTTTTGACCTATGTGGGAAACAGAATTTTCAATGAGTTATGGCAACTTGGCCGAGTGGTTAAGGCGAAAGATTAGAAATCTTTTGGGCTTTGCCCGCGCAGGTTCGAGTCCTGCAGTTGTCGTTATTTTTCTCTTTTTTTTCAATTTCCCTTGTTCGTCAGATCGAGGCGGTAGAAGAAACAATTACTTTTCCTAAATGGGTAAAAACTCGTGTTTTAGGAAAAAAAAGAAAAATTTGGTCAAAACTCGAAAGATAGGTTCTTAATCTTCTTTCAAGTTGAAAAGGCCTACGCTCTTTTCCTTGAAGCATTTTCATCCTACTGCTCGTATTGAACTCCACTATAAGCGCACCAAAAAGATACAAACGTCAATTATGGAAGGCTCAACGGGATTTGATGGAGACGCTACTACTTTTTTCGCTCCAGACGCTGTGTTTGGTGACAGAGTGCGCAGATTTCAAGAGTTTTTAGATACTTTCACCTCATACAGAGACTCTGTAAGGTCCATACAAGTTTACAACAGCAATAACGCGGCCAACTACAACGATGATCAAGATGACGCAGACGAACGAGATTTGCTAGGTGATGACGACGGTGATGATCTTGAAAAGGAAAAGAAAGCAGCATCGTCCACCTCATTGAATATACTCCCTCACAGGATTATCATCTCGCTTGATGACTTGAGAGAATTCGACAGGTCGTTCTGGTCGGGCATTTTAGTCGAACCAGCATACTTCATCCCGCCTGCCGAAAAGGCGCTTACTGACCTAGCAGATTCCATGGACGATGTTCCACATCCCAATGCCTCTGCAGTATCGTCTCGCCATCCTTGGAAGCTTTCGTTCAAAGGCTCATTTGGTGCACACGCATTGTCTCCTCGTACTCTAACGGCACAACATTTAAACAAACTGGTCTCTGTTGAGGGTATCGTAACTAAGACTTCGTTGGTCAGGCCAAAGCTTATCAGATCTGTCCACTACGCGGCAAAGACTGGTAGATTCCATTACAGAGATTATACAGATGCTACTACAACTCTCACCACCCGCATCCCAACGCCTGCCATCTATCCAACGGAGGACACTGAAGGTAACAAACTAACCACCGAATATGGGTATAGTACGTTCATAGACCATCAGCGTATCACTGTGCAAGAAATGCCCGAAATGGCCCCCGCTGGCCAACTTCCCAGGTCCATTGACGTCATTCTCGATGACGACCTTGTGGACAAGACCAAGCCAGGTGACAGAGTTAACGTTGTCGGGGTATTCAAGTCGCTTGGTGCTGGTGGCATGAACCAGTCCAACTCTAATACATTGATCGGGTTCAAAACTCTGATCCTAGGTAATACGGTGTATCCTCTCCACGCCAGATCCACGGGTGTCGCTGCGAGACAAATGTTGACAGATTTCGATATAAGAAATATCAATAAACTATCCAAAAAAAAGGACATTTTCGATATCTTGTCTCAATCTTTAGCGCCTTCTATTTATGGACATGACCATATAAAGAAAGCCATTTTATTGATGCTCATGGGAGGTGTGGAGAAAAATTTAGAAAATGGCTCGCATTTAAGAGGTGACATCAATATCCTAATGGTGGGTGATCCATCCACTGCCAAGTCCCAATTGCTAAGGTTTGTGTTGAATACAGCATCACTGGCAATTGCTACTACTGGTAGAGGTTCTTCCGGTGTCGGTTTGACCGCAGCGGTCACTACTGATAGGGAAACAGGTGAAAGAAGACTAGAGGCTGGTGCCATGGTTCTTGCTGACCGCGGGGTTGTATGTATTGATGAATTTGATAAGATGACAGATGTGGATAGAGTCGCCATTCATGAAGTAATGGAACAACAAACGGTGACGATTGCCAAAGCAGGTATTCACACAACATTAAATGCTCGTTGTAGTGTTATTGCTGCCGCAAATCCCGTTTTTGGGCAGTACGATGTCAATAGAGATCCACACCAAAACATTGCCCTACCGGACTCGCTGTTGTCTCGTTTTGATTTACTATTTGTTGTGACAGACGATATCAATGAAATCAGAGATAGATCCATTAGTGAGCATGTCTTAAGAACACACAGATATTTGCCTCCAGGTTATTTAGAGGGTGAACCTGTGAGAGAGCGTTTGAATTTATCATTAGCCGTTGGGGAGGATGCAGATATAAATCCTGAAGAGCATTCCAACTCCGGGGCTGGTGTAGAAAATGAAGGAGAAGATGATGAAGACCATGTCTTCGAAAAGTTCAACCCCTTATTACAAGCAGGTGCTAAGTTAGCAAAAAACAAAGGTAACTATAACGGTACAGAAATTCCAAAGCTAGTCACCATCCCATTCTTAAGAAAGTACGTTCAATATGCCAAGGAAAGGGTTATTCCACAGTTAACACAAGAAGCCATCAATGTTATTGTGAAAAATTATACTGATTTAAGAAACGATGATAATACCAAAAAATCGCCCATTACTGCAAGAACTTTGGAGACTTTGATCAGATTAGCCACAGCTCACGCCAAAGTCAGGTTATCCAAAACAGTCAACAAGGTGGATGCTAAAGTGGCTGCCAATCTACTAAGGTTTGCACTATTGGGTGAGGATATCGGCAATGATATCGATGAAGAGGAAAGTGAATACGAAGAAGCTTTGTCGAAGAGGTCTCCACAGAAATCACCGAAAAAAAGACAAAGAGTCAGACAACCAGCAAGCAACTCTGGATCCCCAATCAAATCTACTCCAAGAAGGTCAACGGCATCTTCCGTTAATGCCACGCCATCGTCAGCACGCAGAATATTACGTTTTCAAGATGACGAACAGAACGCTGGTGAAGACGATAACGATATAATGTCACCGCTTCCTGCGGATGAGGAAGCTGAATTACAAAGAAGGCTTCAACTGGGGTTGAGAGTGTCTCCAAGACGTAGAGAACATCTTCACGCACCTGAGGAAGGTTCGTCGGGACCTCTTACCGAGGTCGGTACTCCAAGATTACCTAACGTATCTTCTGCAGGTCAGGATGATGAGCAACAACAGTCAGTTATTTCTTTTGACAATGTGGAGCCTGGTACCATTTCTACTGGTAGATTGTCTTTAATCTCAGGTATTATTGCGCGTCTGATGCAAACAGAAATATTTGAAGAAGAATCCTATCCTGTGGCCTCTTTGTTCGAAAGAATCAACGAAGAACTACCGGAGGAGGAAAAATTCTCCGCTCAAGAATATTTAGCAGGTTTGAAGATCATGTCGGACAGAAATAACTTAATGGTTGCTGACGATAAAGTTTGGAGAGTCTGAGCGGCCGC

Amino Acid Sequence

MEGSTGFDGDATTFFAPDAVFGDRVRRFQEFLDTFTSYRDSVRSIQVYNSNNAANYNDDQDDADERDLLGDDDGDDLEKEKKAASSTSLNILPHRIIISLDDLREFDRSFWSGILVEPAYFIPPAEKALTDLADSMDDVPHPNASAVSSRHPWKLSFKGSFGAHALSPRTLTAQHLNKLVSVEGIVTKTSLVRPKLIRSVHYAAKTGRFHYRDYTDATTTLTTRIPTPAIYPTEDTEGNKLTTEYGYSTFIDHQRITVQEMPEMAPAGQLPRSIDVILDDDLVDKTKPGDRVNVVGVFKSLGAGGMNQSNSNTLIGFKTLILGNTVYPLHARSTGVAARQMLTDFDIRNINKLSKKKDIFDILSQSLAPSIYGHDHIKKAILLMLMGGVEKNLENGSHLRGDINILMVGDPSTAKSQLLRFVLNTASLAIATTGRGSSGVGLTAAVTTDRETGERRLEAGAMVLADRGVVCIDEFDKMTDVDRVAIHEVMEQQTVTIAKAGIHTTLNARCSVIAAANPVFGQYDVNRDPHQNIALPDSLLSRFDLLFVVTDDINEIRDRSISEHVLRTHRYLPPGYLEGEPVRERLNLSLAVGEDADINPEEHSNSGAGVENEGEDDEDHVFEKFNPLLQAGAKLAKNKGNYNGTEIPKLVTIPFLRKYVQYAKERVIPQLTQEAINVIVKNYTDLRNDDNTKKSPITARTLETLIRLATAHAKVRLSKTVNKVDAKVAANLLRFALLGEDIGNDIDEEESEYEEALSKRSPQKSPKKRQRVRQPASNSGSPIKSTPRRSTASSVNATPSSARRILRFQDDEQNAGEDDNDIMSPLPADEEAELQRRLQLGLRVSPRRREHLHAPEEGSSGPLTEVGTPRLPNVSSAGQDDEQQQSVISFDNVEPGTISTGRLSLISGIIARLMQTEIFEEESYPVASLFERINEELPEEEKFSAQEYLAGLKIMSDRNNLMVADDKVWRVAAAHHHHHHGEQKLISEEDLNGEQKLISEEDLNGLDGEQKLISEEDLG*

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU19080
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Yeast vector to express MCM3 with c-term 6xHIS and 3xMyc tags.