cDNA Clone RFA2 (yeast)

• Plasmid: pSC-B-RFA2
• Parent Plasmid: pRS415
• Genbank: NP_014087
• Species: Yeast

Sequence of Insert

GGATCCCCATAAATTCGTAGGCAGGCTGGCCCATAATGGCTAATGAGCGGGAAAGGGCTGTATTAACTGCTCCTATTTCAATTCCAGTTTTGCCAAACAAACCTGAGATTGCTTTCAACCTAGCTGCTGGGCCGGTGTAGTTATTTTGAATAAAGGTCTGTAAGAGGGCAATAGCCAAAAATAGCATCTCATTTTCTTGGCCCATTTTGAAAACCTCAACGTTAAGAAAGTCATTTAGAGCAGCAATAACACTAGATGTGGATATTTCTCCCGAAATACTAATTTTTCCCTTAAATTTGTCCACAAATTGCTGTACTAGCTGGTATGATTCACCACTGATGATATGTTCAGCCTTTTTCACATATGTCTGGTCGAAAGAACCACTTTTCAGAGATTCTACTTCTGCAGACAGCAATAATTTTGCATGCAATAACGTCTCCATAATTTTGCAACCACTAGTTTACTTTTTGCCTGGTCTGATAGCTTCTTTTTAGCAATTCCTTTGGCCTCGATGAGCTTCCATTTTCAATTTTCATCTTTTTTTTCACGATGCGAAGTTTCTTAGATAATTATCGCCATATACGAAACGCGTTAGGAAACGCGTTCTTTCCTTTATAATAAGCAATATTATATCAACTTAAGGTGGAAGTAATGTGGCAAAATCGATAGCGACTATCTAGAACAGGCTAGTTTAAGCATATACATAATGGCAACCTATCAACCATATAACGAATATTCATCAGTAACGGGCGGTGGCTTTGAGAACTCTGAGTCCCGCCCAGGTAGTGGGGAGTCGGAAACTAACACTAGAGTTAACACCTTGACACCTGTGACGATCAAACAAATTCTAGAGTCCAAACAGGATATTCAGGACGGCCCCTTCGTTTCGCATAACCAAGAACTTCATCACGTTTGTTTTGTAGGTGTGGTGAGAAACATTACAGACCATACTGCAAATATTTTTTTAACTATTGAGGATGGAACTGGTCAAATAGAAGTGAGAAAATGGAGCGAAGATGCAAATGACTTGGCTGCCGGTAACGATGACTCTTCTGGTAAAGGTTATGGTTCGCAAGTCGCCCAACAATTTGAAATTGGCGGTTACGTAAAAGTTTTTGGTGCTTTGAAAGAGTTTGGTGGTAAGAAAAATATACAGTATGCGGTGATTAAGCCCATAGATTCATTCAATGAAGTGTTGACGCATCACTTGGAAGTCATCAAATGTCATTCCATAGCCAGTGGAATGATGAAACAACCTTTGGAGAGTGCATCCAACAACAATGGGCAATCATTATTTGTCAAGGATGATAACGATACATCTTCCGGCTCCAGTCCGTTACAAAGAATTCTAGAATTTTGTAAGAAGCAATGTGAGGGCAAAGACGCTAATTCATTCGCTGTTCCCATTCCATTGATCTCGCAATCCTTGAATTTGGATGAAACTACCGTCAGAAACTGCTGTACGACCTTGACTGACCAGGGTTTTATCTACCCAACTTTTGATGACAATAACTTCTTTGCCCTATGAGCGGCCGC

Amino Acid Sequence

MATYQPYNEYSSVTGGGFENSESRPGSGESETNTRVNTLTPVTIKQILESKQDIQDGPFVSHNQELHHVCFVGVVRNITDHTANIFLTIEDGTGQIEVRKWSEDANDLAAGNDDSSGKGYGSQVAQQFEIGGYVKVFGALKEFGGKKNIQYAVIKPIDSFNEVLTHHLEVIKCHSIASGMMKQPLESASNNNGQSLFVKDDNDTSSGSSPLQRILEFCKKQCEGKDANSFAVPIPLISQSLNLDETTVRNCCTTLTDQGFIYPTFDDNNFFALAAAHHHHHHGEQKLISEEDLNGEQKLISEEDLNGLDGEQKLISEEDLG*

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU19076
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Yeast vector to express RFA2 with c-term 6xHIS and 3xMyc tags.