cDNA Clone LCK

• Plasmid: pFB HT LCK
• Parent Plasmid: pFastBac HTb
• Expressed: 6xHis-LCK
• Genbank: BC013200.1
• Tag: 6His
• Species: Human

Sequence of Insert

gaattcGGCTGTGGCTGCAGCTCACACCCGGAAGATGACTGGATGGAAAA
CATCGATGTGTGTGAGAACTGCCATTATCCCATAGTCCCACTGGATGGCA
AGGGCACGCTGCTCATCCGAAATGGCTCTGAGGTGCGGGACCCACTGGTT
ACCTACGAAGGCTCCAATCCGCCGGCTTCCCCACTGCAAGACAACCTGGT
TATCGCTCTGCACAGCTATGAGCCCTCTCACGACGGAGATCTGGGCTTTG
AGAAGGGGGAACAGCTCCGCATCCTGGAGCAGAGCGGCGAGTGGTGGAAG
GCGCAGTCCCTGACCACGGGCCAGGAAGGCTTCATCCCCTTCAATTTTGT
GGCCAAAGCGAACAGCCTGGAGCCCGAACCCTGGTTCTTCAAGAACCTGA
GCCGCAAGGACGCGGAGCGGCAGCTCCTGGCGCCCGGGAACACTCACGGC
TCCTTCCTCATCCGGGAGAGCGAGAGCACCGCGGGATCGTTTTCACTGTC
GGTCCGGGACTTCGACCAGAACCAGGGAGAGGTGGTGAAACATTACAAGA
TCCGTAATCTGGACAACGGTGGCTTCTACATCTCCCCTCGAATCACTTTT
CCCGGCCTGCATGAACTGGTCCGCCATTACACCAATGCTTCAGATGGGCT
GTGCACACGGTTGAGCCGCCCCTGCCAGACCCAGAAGCCCCAGAAGCCGT
GGTGGGAGGACGAGTGGGAGGTTCCCAGGGAGACGCTGAAGCTGGTGGAG
CGGCTGGGGGCTGGACAGTTCGGGGAGGTGTGGATGGGGTACTACAACGG
GCACACGAAGGTGGCGGTGAAGAGCCTGAAGCAGGGCAGCATGTCCCCGG
ACGCCTTCCTGGCCGAGGCCAACCTCATGAAGCAGCTGCAACACCAGCGG
CTGGTTCGGCTCTACGCTGTGGTCACCCAGGAGCCCATCTACATCATCAC
TGAATACATGGAGAATGACACACTTCTAGACTCCCAGTTGGAGGAGAAAG
GTCTGGGGGCCTCCCCCTGGGGCAACTTGGGCCAGCAACTCTTGCTTCTG
CCCACAGGGAGTCTAGTGGATTTTCTCAAGACCCCTTCAGGCATCAAGTT
GACCATCAACAAACTCCTGGACATGGCAGCCCAAATTGCAGAAGGCATGG
CATTCATTGAAGAGCGGAATTATATTCATCGTGACCTTCGGGCTGCCAAC
ATTCTGGTGTCTGACACCCTGAGCTGCAAGATTGCAGACTTTGGCCTAGC
ACGCCTCATTGAGGACAACGAGTACACAGCCAGGGAGGGGGCCAAGTTTC
CCATTAAGTGGACAGCGCCAGAAGCCATTAACTACGGGACATTCACCATC
AAGTCAGATGTGTGGTCTTTTGGGATCCTGCTGACGGAAATTGTCACCCA
CGGCCGCATCCCTTACCCAGGGATGACCAACCCGGAGGTGATTCAGAACC
TGGAGCGAGGCTACCGCATGGTGCGCCCTGACAACTGTCCAGAGGAGCTG
TACCAACTCATGAGGCTGTGCTGGAAGGAGCGCCCAGAGGACCGGCCCAC
CTTTGACTACCTGCGCAGTGTGCTGGAGGACTTCTTCACGGCCACAGAGG
GCCAGTACCAGCCTCAGCCTTGAgcggccgc

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMDPEFGCGCSSHPEDDWMENIDVCENCHYPIVPLDGKGTLLIRNGSEVRDPLV
TYEGSNPPASPLQDNLVIALHSYEPSHDGDLGFEKGEQLRILEQSGEWWK
AQSLTTGQEGFIPFNFVAKANSLEPEPWFFKNLSRKDAERQLLAPGNTHG
SFLIRESESTAGSFSLSVRDFDQNQGEVVKHYKIRNLDNGGFYISPRITF
PGLHELVRHYTNASDGLCTRLSRPCQTQKPQKPWWEDEWEVPRETLKLVE
RLGAGQFGEVWMGYYNGHTKVAVKSLKQGSMSPDAFLAEANLMKQLQHQR
LVRLYAVVTQEPIYIITEYMENDTLLDSQLEEKGLGASPWGNLGQQLLLL
PTGSLVDFLKTPSGIKLTINKLLDMAAQIAEGMAFIEERNYIHRDLRAAN
ILVSDTLSCKIADFGLARLIEDNEYTAREGAKFPIKWTAPEAINYGTFTI
KSDVWSFGILLTEIVTHGRIPYPGMTNPEVIQNLERGYRMVRPDNCPEEL
YQLMRLCWKERPEDRPTFDYLRSVLEDFFTATEGQYQPQP*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU19045
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N Ter 6HIS for baculovirus expression.