cDNA Clone AOX1

• Plasmid: pCMV-FLAG-2 AOX1
• Parent Plasmid: pCMV5 FLAG2
• Expressed: FLAG AOX1
• Genbank: NM_001159.3
• Tag: FLAG
• Species: Human

Sequence of Insert

GCGGCCGCGATGGACCGGGCGTCCGAGCTGCTCTTCTACGTGAACGGCCG
CAAGGTGATAGAAAAAAATGTCGATCCTGAAACAATGCTGTTGCCTTATT
TGAGGAAGAAGCTTCGACTCACAGGAACTAAGTATGGCTGTGGAGGAGGA
GGCTGTGGTGCTTGTACAGTGATGATATCACGATACAACCCCATCACCAA
GAGGATAAGGCATCACCCAGCCAATGCCTGTCTGATTCCCATCTGTTCTC
TGTATGGTGCTGCCGTCACCACAGTAGAAGGCATAGGAAGCACCCACACC
AGAATTCATCCTGTTCAGGAGAGGATTGCCAAGTGTCATGGCACCCAGTG
TGGCTTCTGCACACCTGGGATGGTGATGTCCATCTACACGCTGCTCAGGA
ACCACCCAGAGCCCACTCTGGATCAGTTAACTGATGCCCTTGGTGGTAAC
CTGTGCCGTTGCACTGGATACAGGCCCATAATTGATGCATGCAAGACTTT
CTGTAAAACTTCGGGCTGCTGTCAAAGTAAAGAAAATGGGGTTTGCTGTT
TGGATCAAGGAATCAATGGATTGCCAGAATTTGAGGAAGGAAGTAAGACA
AGTCCAAAACTCTTCGCAGAAGAGGAGTTTCTGCCATTGGATCCAACCCA
GGAACTGATATTTCCTCCTGAGCTAATGATAATGGCTGAGAAACAGTCGC
AAAGGACCAGGGTGTTTGGCAGTGAGAGAATGATGTGGTTTTCCCCCGTG
ACCCTGAAGGAACTGCTGGAATTTAAATTCAAGTATCCCCAGGCTCCTGT
TATCATGGGAAACACCTCTGTGGGGCCTGAAGTGAAATTTAAAGGCGTCT
TTCACCCAGTTATAATTTCTCCTGATAGAATTGAAGAACTGAGTGTTGTA
AACCATGCATATAATGGACTCACCCTTGGTGCTGGTCTCAGCCTAGCCCA
GGTGAAGGACATTTTGGCTGATGTAGTCCAGAAGCTTCCAGAGGAGAAGA
CACAGATGTACCATGCTCTCCTGAAGCATTTGGGAACTCTGGCTGGGTCC
CAGATCAGGAACATGGCTTCTTTAGGGGGACACATCATTAGCAGGCATCC
AGATTCAGATCTGAATCCCATCCTGGCTGTGGGTAACTGTACCCTCAACT
TGCTATCAAAAGAAGGAAAACGACAGATTCCTTTAAATGAGCAATTCCTC
AGCAAGTGCCCTAATGCAGATCTTAAGCCTCAAGAAATCTTGGTCTCAGT
GAACATCCCCTACTCAAGGAAGTGGGAATTTGTGTCAGCCTTCCGACAAG
CCCAGCGACAGGAGAATGCGCTAGCGATAGTCAATTCAGGAATGAGAGTC
TTTTTTGGAGAAGGGGATGGCATTATTAGAGAGTTATGCATCTCATATGG
AGGCGTTGGTCCAGCCACCATCTGTGCCAAGAATTCCTGCCAGAAACTCA
TTGGAAGGCACTGGAACGAACAGATGCTGGATATAGCCTGCAGGCTTATT
CTGAATGAAGTCTCCCTTTTGGGCTCGGCGCCAGGTGGGAAAGTGGAGTT
CAAGAGGACTCTCATCATCAGCTTCCTCTTCAAGTTCTACCTGGAAGTGT
CACAGATTTTGAAAAAGATGGATCCAGTTCACTATCCTAGCCTTGCAGAC
AAGTATGAAAGTGCTTTAGAAGATCTTCATTCCAAACATCACTGCAGTAC
ATTAAAGTACCAGAATATAGGCCCAAAGCAGCATCCTGAAGACCCAATTG
GCCACCCCATCATGCATCTGTCTGGTGTGAAGCATGCCACGGGGGAGGCC
ATCTACTGTGATGACATGCCTCTGGTGGACCAGGAACTTTTCTTGACTTT
TGTGACTAGTTCAAGAGCTCATGCTAAGATTGTGTCTATTGATCTGTCAG
AAGCTCTCAGCATGCCCGGTGTGGTGGACATCATGACAGCAGAACATCTT
AGTGACGTCAACTCCTTCTGCTTTTTTACTGAAGCTGAGAAATTTCTGGC
GACAGATAAGGTGTTCTGTGTGGGTCAGCTTGTCTGTGCTGTGCTTGCCG
ATTCTGAGGTTCAGGCAAAGCGAGCTGCTAAGCGAGTGAAGATTGTCTAT
CAAGACTTGGAGCCGCTGATACTAACAATTGAGGAAAGTATACAACACAA
CTCCTCCTTCAAGCCAGAAAGGAAACTGGAATATGGAAATGTTGACGAAG
CATTTAAAGTGGTTGATCAAATTCTTGAAGGTGAAATACATATGGGAGGT
CAAGAACATTTTTATATGGAAACCCAAAGCATGCTTGTCGTTCCCAAGGG
AGAGGATCAAGAAATGGATGTCTACGTGTCCACACAGTTTCCCAAATATA
TACAGGACATTGTTGCCTCAACCTTGAAGCTCCCAGCTAACAAGGTCATG
TGCCATGTAAGGCGTGTTGGTGGAGCGTTTGGAGGGAAGGTGTTAAAAAC
CGGAATCATTGCAGCCGTCACTGCATTTGCCGCAAACAAACATGGCCGTG
CAGTTCGCTGTGTTCTGGAACGAGGAGAAGACATGTTAATAACTGGAGGC
CGCCATCCTTACCTTGGAAAGTACAAAGCTGGATTCATGAACGATGGCAG
AATCTTGGCCCTGGACATGGAGCATTACAGCAATGCAGGCGCCTCCTTGG
ATGAATCATTATTCGTGATAGAAATGGGACTTCTGAAAATGGACAATGCT
TACAAGTTTCCCAATCTCCGCTGCCGGGGTTGGGCATGCAGAACCAACCT
TCCATCCAACACAGCTTTTCGTGGGTTTGGCTTTCCTCAGGCAGCGCTGA
TCACCGAATCTTGTATCACGGAAGTTGCAGCCAAATGTGGACTATCCCCT
GAGAAGGTGCGAATCATAAACATGTACAAGGAAATTGATCAAACACCCTA
CAAACAAGAGATCAATGCCAAGAACCTAATCCAGTGTTGGAGAGAATGTA
TGGCCATGTCTTCCTACTCCTTGAGGAAAGTTGCTGTGGAAAAGTTCAAT
GCAGAGAATTATTGGAAGAAGAAAGGACTGGCCATGGTCCCCCTGAAGTT
TCCTGTTGGCCTTGGCTCACGTGCTGCTGGTCAGGCTGCTGCCTTGGTTC
ACATTTATCTTGATGGCTCTGTGCTGGTCACTCACGGTGGAATTGAAATG
GGGCAGGGGGTCCACACTAAAATGATTCAGGTGGTCAGCCGTGAATTAAG
AATGCCAATGTCGAATGTCCACCTGCGTGGAACAAGCACAGAAACTGTCC
CTAATGCAAATATCTCTGGAGGTTCTGTGGTGGCAGATCTCAACGGTTTG
GCAGTAAAGGATGCCTGTCAAACTCTTCTAAAACGCCTCGAACCCATCAT
CAGCAAGAATCCTAAAGGAACTTGGAAAGACTGGGCACAGACTGCTTTTG
ATGAAAGCATTAACCTTTCAGCTGTTGGATACTTCAGAGGTTATGAGTCA
GACATGAACTGGGAGAAAGGCGAAGGCCAGCCCTTCGAATACTTTGTTTA
TGGAGCTGCCTGTTCCGAGGTTGAAATAGACTGCCTGACGGGGGATCATA
AGAACATCAGAACAGACATTGTCATGGATGTTGGCTGCAGTATAAATCCA
GCCATTGACATAGGCCAGATTGAAGGTGCATTTATTCAAGGCATGGGACT
TTATACAATAGAGGAACTGAATTATTCTCCCCAGGGCATTCTGCACACTC
GTGGTCCAGACCAATATAAAATCCCTGCCATCTGTGACATGCCCACGGAG
TTGCACATTGCTTTGTTGCCTCCTTCTCAAAACTCAAATACTCTTTATTC
ATCTAAGGGTCTGGGAGAGTCGGGGGTGTTCCTGGGGTGTTCCGTGTTTT
TCGCTATCCATGACGCAGTGAGTGCAGCACGACAGGAGAGAGGCCTGCAT
GGACCCTTGACCCTTAATAGTCCACTGACCCCGGAGAAGATTAGGATGGC
CTGTGAAGACAAGTTCACAAAAATGATTCCGAGAGATGAACCTGGATCCT
ACGTTCCTTGGAATGTACCCATCTGAGCGGCCGC

Amino Acid Sequence

MDYKDDDDKGIPGIPGSTRAAAMDRASELLFYVNGRKVIEKNVDPETMLL
PYLRKKLRLTGTKYGCGGGGCGACTVMISRYNPITKRIRHHPANACLIPI
CSLYGAAVTTVEGIGSTHTRIHPVQERIAKCHGTQCGFCTPGMVMSIYTL
LRNHPEPTLDQLTDALGGNLCRCTGYRPIIDACKTFCKTSGCCQSKENGV
CCLDQGINGLPEFEEGSKTSPKLFAEEEFLPLDPTQELIFPPELMIMAEK
QSQRTRVFGSERMMWFSPVTLKELLEFKFKYPQAPVIMGNTSVGPEVKFK
GVFHPVIISPDRIEELSVVNHAYNGLTLGAGLSLAQVKDILADVVQKLPE
EKTQMYHALLKHLGTLAGSQIRNMASLGGHIISRHPDSDLNPILAVGNCT
LNLLSKEGKRQIPLNEQFLSKCPNADLKPQEILVSVNIPYSRKWEFVSAF
RQAQRQENALAIVNSGMRVFFGEGDGIIRELCISYGGVGPATICAKNSCQ
KLIGRHWNEQMLDIACRLILNEVSLLGSAPGGKVEFKRTLIISFLFKFYL
EVSQILKKMDPVHYPSLADKYESALEDLHSKHHCSTLKYQNIGPKQHPED
PIGHPIMHLSGVKHATGEAIYCDDMPLVDQELFLTFVTSSRAHAKIVSID
LSEALSMPGVVDIMTAEHLSDVNSFCFFTEAEKFLATDKVFCVGQLVCAV
LADSEVQAKRAAKRVKIVYQDLEPLILTIEESIQHNSSFKPERKLEYGNV
DEAFKVVDQILEGEIHMGGQEHFYMETQSMLVVPKGEDQEMDVYVSTQFP
KYIQDIVASTLKLPANKVMCHVRRVGGAFGGKVLKTGIIAAVTAFAANKH
GRAVRCVLERGEDMLITGGRHPYLGKYKAGFMNDGRILALDMEHYSNAGA
SLDESLFVIEMGLLKMDNAYKFPNLRCRGWACRTNLPSNTAFRGFGFPQA
ALITESCITEVAAKCGLSPEKVRIINMYKEIDQTPYKQEINAKNLIQCWR
ECMAMSSYSLRKVAVEKFNAENYWKKKGLAMVPLKFPVGLGSRAAGQAAA
LVHIYLDGSVLVTHGGIEMGQGVHTKMIQVVSRELRMPMSNVHLRGTSTE
TVPNANISGGSVVADLNGLAVKDACQTLLKRLEPIISKNPKGTWKDWAQT
AFDESINLSAVGYFRGYESDMNWEKGEGQPFEYFVYGAACSEVEIDCLTG
DHKNIRTDIVMDVGCSINPAIDIGQIEGAFIQGMGLYTIEELNYSPQGIL
HTRGPDQYKIPAICDMPTELHIALLPPSQNSNTLYSSKGLGESGVFLGCS
VFFAIHDAVSAARQERGLHGPLTLNSPLTPEKIRMACEDKFTKMIPRDEP
GSYVPWNVPI*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU35464
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter FLAG for mammalian expression.; ; Estimated MW of expressed protein : 150188 Daltons