cDNA Clone AOX1

• Plasmid: pCMV-HA-2 AOX1
• Parent Plasmid: pCMV5 HA2
• Expressed: HA AOX1
• Genbank: NM_001159.3
• Tag: HA
• Species: Human

Sequence of Insert

GCGGCCGCGATGGACCGGGCGTCCGAGCTGCTCTTCTACGTGAACGGCCG
CAAGGTGATAGAAAAAAATGTCGATCCTGAAACAATGCTGTTGCCTTATT
TGAGGAAGAAGCTTCGACTCACAGGAACTAAGTATGGCTGTGGAGGAGGA
GGCTGTGGTGCTTGTACAGTGATGATATCACGATACAACCCCATCACCAA
GAGGATAAGGCATCACCCAGCCAATGCCTGTCTGATTCCCATCTGTTCTC
TGTATGGTGCTGCCGTCACCACAGTAGAAGGCATAGGAAGCACCCACACC
AGAATTCATCCTGTTCAGGAGAGGATTGCCAAGTGTCATGGCACCCAGTG
TGGCTTCTGCACACCTGGGATGGTGATGTCCATCTACACGCTGCTCAGGA
ACCACCCAGAGCCCACTCTGGATCAGTTAACTGATGCCCTTGGTGGTAAC
CTGTGCCGTTGCACTGGATACAGGCCCATAATTGATGCATGCAAGACTTT
CTGTAAAACTTCGGGCTGCTGTCAAAGTAAAGAAAATGGGGTTTGCTGTT
TGGATCAAGGAATCAATGGATTGCCAGAATTTGAGGAAGGAAGTAAGACA
AGTCCAAAACTCTTCGCAGAAGAGGAGTTTCTGCCATTGGATCCAACCCA
GGAACTGATATTTCCTCCTGAGCTAATGATAATGGCTGAGAAACAGTCGC
AAAGGACCAGGGTGTTTGGCAGTGAGAGAATGATGTGGTTTTCCCCCGTG
ACCCTGAAGGAACTGCTGGAATTTAAATTCAAGTATCCCCAGGCTCCTGT
TATCATGGGAAACACCTCTGTGGGGCCTGAAGTGAAATTTAAAGGCGTCT
TTCACCCAGTTATAATTTCTCCTGATAGAATTGAAGAACTGAGTGTTGTA
AACCATGCATATAATGGACTCACCCTTGGTGCTGGTCTCAGCCTAGCCCA
GGTGAAGGACATTTTGGCTGATGTAGTCCAGAAGCTTCCAGAGGAGAAGA
CACAGATGTACCATGCTCTCCTGAAGCATTTGGGAACTCTGGCTGGGTCC
CAGATCAGGAACATGGCTTCTTTAGGGGGACACATCATTAGCAGGCATCC
AGATTCAGATCTGAATCCCATCCTGGCTGTGGGTAACTGTACCCTCAACT
TGCTATCAAAAGAAGGAAAACGACAGATTCCTTTAAATGAGCAATTCCTC
AGCAAGTGCCCTAATGCAGATCTTAAGCCTCAAGAAATCTTGGTCTCAGT
GAACATCCCCTACTCAAGGAAGTGGGAATTTGTGTCAGCCTTCCGACAAG
CCCAGCGACAGGAGAATGCGCTAGCGATAGTCAATTCAGGAATGAGAGTC
TTTTTTGGAGAAGGGGATGGCATTATTAGAGAGTTATGCATCTCATATGG
AGGCGTTGGTCCAGCCACCATCTGTGCCAAGAATTCCTGCCAGAAACTCA
TTGGAAGGCACTGGAACGAACAGATGCTGGATATAGCCTGCAGGCTTATT
CTGAATGAAGTCTCCCTTTTGGGCTCGGCGCCAGGTGGGAAAGTGGAGTT
CAAGAGGACTCTCATCATCAGCTTCCTCTTCAAGTTCTACCTGGAAGTGT
CACAGATTTTGAAAAAGATGGATCCAGTTCACTATCCTAGCCTTGCAGAC
AAGTATGAAAGTGCTTTAGAAGATCTTCATTCCAAACATCACTGCAGTAC
ATTAAAGTACCAGAATATAGGCCCAAAGCAGCATCCTGAAGACCCAATTG
GCCACCCCATCATGCATCTGTCTGGTGTGAAGCATGCCACGGGGGAGGCC
ATCTACTGTGATGACATGCCTCTGGTGGACCAGGAACTTTTCTTGACTTT
TGTGACTAGTTCAAGAGCTCATGCTAAGATTGTGTCTATTGATCTGTCAG
AAGCTCTCAGCATGCCCGGTGTGGTGGACATCATGACAGCAGAACATCTT
AGTGACGTCAACTCCTTCTGCTTTTTTACTGAAGCTGAGAAATTTCTGGC
GACAGATAAGGTGTTCTGTGTGGGTCAGCTTGTCTGTGCTGTGCTTGCCG
ATTCTGAGGTTCAGGCAAAGCGAGCTGCTAAGCGAGTGAAGATTGTCTAT
CAAGACTTGGAGCCGCTGATACTAACAATTGAGGAAAGTATACAACACAA
CTCCTCCTTCAAGCCAGAAAGGAAACTGGAATATGGAAATGTTGACGAAG
CATTTAAAGTGGTTGATCAAATTCTTGAAGGTGAAATACATATGGGAGGT
CAAGAACATTTTTATATGGAAACCCAAAGCATGCTTGTCGTTCCCAAGGG
AGAGGATCAAGAAATGGATGTCTACGTGTCCACACAGTTTCCCAAATATA
TACAGGACATTGTTGCCTCAACCTTGAAGCTCCCAGCTAACAAGGTCATG
TGCCATGTAAGGCGTGTTGGTGGAGCGTTTGGAGGGAAGGTGTTAAAAAC
CGGAATCATTGCAGCCGTCACTGCATTTGCCGCAAACAAACATGGCCGTG
CAGTTCGCTGTGTTCTGGAACGAGGAGAAGACATGTTAATAACTGGAGGC
CGCCATCCTTACCTTGGAAAGTACAAAGCTGGATTCATGAACGATGGCAG
AATCTTGGCCCTGGACATGGAGCATTACAGCAATGCAGGCGCCTCCTTGG
ATGAATCATTATTCGTGATAGAAATGGGACTTCTGAAAATGGACAATGCT
TACAAGTTTCCCAATCTCCGCTGCCGGGGTTGGGCATGCAGAACCAACCT
TCCATCCAACACAGCTTTTCGTGGGTTTGGCTTTCCTCAGGCAGCGCTGA
TCACCGAATCTTGTATCACGGAAGTTGCAGCCAAATGTGGACTATCCCCT
GAGAAGGTGCGAATCATAAACATGTACAAGGAAATTGATCAAACACCCTA
CAAACAAGAGATCAATGCCAAGAACCTAATCCAGTGTTGGAGAGAATGTA
TGGCCATGTCTTCCTACTCCTTGAGGAAAGTTGCTGTGGAAAAGTTCAAT
GCAGAGAATTATTGGAAGAAGAAAGGACTGGCCATGGTCCCCCTGAAGTT
TCCTGTTGGCCTTGGCTCACGTGCTGCTGGTCAGGCTGCTGCCTTGGTTC
ACATTTATCTTGATGGCTCTGTGCTGGTCACTCACGGTGGAATTGAAATG
GGGCAGGGGGTCCACACTAAAATGATTCAGGTGGTCAGCCGTGAATTAAG
AATGCCAATGTCGAATGTCCACCTGCGTGGAACAAGCACAGAAACTGTCC
CTAATGCAAATATCTCTGGAGGTTCTGTGGTGGCAGATCTCAACGGTTTG
GCAGTAAAGGATGCCTGTCAAACTCTTCTAAAACGCCTCGAACCCATCAT
CAGCAAGAATCCTAAAGGAACTTGGAAAGACTGGGCACAGACTGCTTTTG
ATGAAAGCATTAACCTTTCAGCTGTTGGATACTTCAGAGGTTATGAGTCA
GACATGAACTGGGAGAAAGGCGAAGGCCAGCCCTTCGAATACTTTGTTTA
TGGAGCTGCCTGTTCCGAGGTTGAAATAGACTGCCTGACGGGGGATCATA
AGAACATCAGAACAGACATTGTCATGGATGTTGGCTGCAGTATAAATCCA
GCCATTGACATAGGCCAGATTGAAGGTGCATTTATTCAAGGCATGGGACT
TTATACAATAGAGGAACTGAATTATTCTCCCCAGGGCATTCTGCACACTC
GTGGTCCAGACCAATATAAAATCCCTGCCATCTGTGACATGCCCACGGAG
TTGCACATTGCTTTGTTGCCTCCTTCTCAAAACTCAAATACTCTTTATTC
ATCTAAGGGTCTGGGAGAGTCGGGGGTGTTCCTGGGGTGTTCCGTGTTTT
TCGCTATCCATGACGCAGTGAGTGCAGCACGACAGGAGAGAGGCCTGCAT
GGACCCTTGACCCTTAATAGTCCACTGACCCCGGAGAAGATTAGGATGGC
CTGTGAAGACAAGTTCACAAAAATGATTCCGAGAGATGAACCTGGATCCT
ACGTTCCTTGGAATGTACCCATCTGAGCGGCCGC

Amino Acid Sequence

MYPYDVPDYAGIPGIPGSTRAAAMDRASELLFYVNGRKVIEKNVDPETML
LPYLRKKLRLTGTKYGCGGGGCGACTVMISRYNPITKRIRHHPANACLIP
ICSLYGAAVTTVEGIGSTHTRIHPVQERIAKCHGTQCGFCTPGMVMSIYT
LLRNHPEPTLDQLTDALGGNLCRCTGYRPIIDACKTFCKTSGCCQSKENG
VCCLDQGINGLPEFEEGSKTSPKLFAEEEFLPLDPTQELIFPPELMIMAE
KQSQRTRVFGSERMMWFSPVTLKELLEFKFKYPQAPVIMGNTSVGPEVKF
KGVFHPVIISPDRIEELSVVNHAYNGLTLGAGLSLAQVKDILADVVQKLP
EEKTQMYHALLKHLGTLAGSQIRNMASLGGHIISRHPDSDLNPILAVGNC
TLNLLSKEGKRQIPLNEQFLSKCPNADLKPQEILVSVNIPYSRKWEFVSA
FRQAQRQENALAIVNSGMRVFFGEGDGIIRELCISYGGVGPATICAKNSC
QKLIGRHWNEQMLDIACRLILNEVSLLGSAPGGKVEFKRTLIISFLFKFY
LEVSQILKKMDPVHYPSLADKYESALEDLHSKHHCSTLKYQNIGPKQHPE
DPIGHPIMHLSGVKHATGEAIYCDDMPLVDQELFLTFVTSSRAHAKIVSI
DLSEALSMPGVVDIMTAEHLSDVNSFCFFTEAEKFLATDKVFCVGQLVCA
VLADSEVQAKRAAKRVKIVYQDLEPLILTIEESIQHNSSFKPERKLEYGN
VDEAFKVVDQILEGEIHMGGQEHFYMETQSMLVVPKGEDQEMDVYVSTQF
PKYIQDIVASTLKLPANKVMCHVRRVGGAFGGKVLKTGIIAAVTAFAANK
HGRAVRCVLERGEDMLITGGRHPYLGKYKAGFMNDGRILALDMEHYSNAG
ASLDESLFVIEMGLLKMDNAYKFPNLRCRGWACRTNLPSNTAFRGFGFPQ
AALITESCITEVAAKCGLSPEKVRIINMYKEIDQTPYKQEINAKNLIQCW
RECMAMSSYSLRKVAVEKFNAENYWKKKGLAMVPLKFPVGLGSRAAGQAA
ALVHIYLDGSVLVTHGGIEMGQGVHTKMIQVVSRELRMPMSNVHLRGTST
ETVPNANISGGSVVADLNGLAVKDACQTLLKRLEPIISKNPKGTWKDWAQ
TAFDESINLSAVGYFRGYESDMNWEKGEGQPFEYFVYGAACSEVEIDCLT
GDHKNIRTDIVMDVGCSINPAIDIGQIEGAFIQGMGLYTIEELNYSPQGI
LHTRGPDQYKIPAICDMPTELHIALLPPSQNSNTLYSSKGLGESGVFLGC
SVFFAIHDAVSAARQERGLHGPLTLNSPLTPEKIRMACEDKFTKMIPRDE
PGSYVPWNVPI*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU35463
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter HA for mammalian expression; ; Estimated MW of expressed protein : 150184 Daltons