cDNA Clone GSK3 alpha

• Synonyms: GSK3A
• Plasmid: pCMV-FLAG-1 GSK3 alpha
• Parent Plasmid: pCMV5 FLAG1
• Expressed: FLAG GSK3 alpha
• Genbank: NP_063937
• Tag: FLAG
• Species: Human

Sequence of Insert

GGATCCCCGGAATTCGCCCTTAGCGGCGGCGGGCCTTCGGGAGGCGGCCCTGGGGGCTCGGGCAGGGCGCGGACTAGCTCGTTCGCGGAGCCCGGCGGCGGAGGCGGAGGAGGCGGCGGCGGCCCCGGAGGCTCGGCCTCCGGCCCAGGCGGCACCGGCGGCGGAAAGGCATCTGTCGGGGCCATGGGTGGGGGCGTCGGGGCCTCGAGCTCCGGGGGTGGACCCGGCGGCAGCGGCGGAGGAGGCAGCGGAGGCCCCGGCGCAGGCACTAGCTTCCCGCCGCCCGGGGTGAAGCTGGGCCGTGACAGCGGGAAGGTGACCACAGTCATAGCCACTCTAGGCCAAGGCCCAGAGCGCTCCCAAGAAGTGGCTTACACGGACATCAAAGTGATTGGCAATGGCTCATTTGGGGTCGTGTACCAGGCACGGCTGGCAGAGACCAGGGAACTAGTCGCCATCAAGAAGGTTCTCCAGGACAAGAGGTTCAAGAACCGAGAGCTGCAGATCATGCGTAAGCTGGACCACTGCAATATTGTGAGGCTGAGATACTTTTTCTACTCCAGTGGCGAGAAGAAAGACGAGCTTTACCTAAATCTGGTGCTGGAATATGTGCCCGAGACAGTGTACCGGGTGGCCCGCCACTTCACCAAGGCCAAGTTGACCATCCCTATCCTCTATGTCAAGGTGTACATGTACCAGCTCTTCCGCAGCTTGGCCTACATCCACTCCCAGGGCGTGTGTCACCGCGACATCAAGCCCCAGAACCTGCTGGTGGACCCTGACACTGCTGTCCTCAAGCTCTGCGATTTTGGCAGTGCAAAGCAGTTGGTCCGAGGGGAGCCCAATGTCTCCTACATCTGTTCTCGCTACTACCGGGCCCCAGAGCTCATCTTTGGAGCCACTGATTACACCTCATCCATCGATGTTTGGTCAGCTGGCTGTGTACTGGCAGAGCTCCTCTTGGGCCAGCCCATCTTCCCTGGGGACAGTGGGGTGGACCAGCTGGTGGAGATCATCAAGGTGCTGGGAACACCAACCCGGGAACAAATCCGAGAGATGAACCCCAACTACACGGAGTTCAAGTTCCCTCAGATTAAAGCTCACCCCTGGACAAAGGTGTTCAAATCTCGAACGCCGCCAGAGGCCATCGCGCTCTGCTCTAGCCTGCTGGAGTACACCCCATCCTCAAGGCTCTCCCCACTAGAGGCCTGTGCGCACAGCTTCTTTGATGAACTGCGATGTCTGGGAACCCAGCTGCCTAACAACCGCCCACTTCCCCCTCTCTTCAACTTCAGTGCTGGTGAACTCTCCATCCAACCGTCTCTCAACGCCATTCTCATCCCTCCTCACTTGAGGTCCCCAGCGGGCACTACCACCCTCACCCCGTCCTCACAAGCTTTAACTGAGACTCCGACCAGCTCAGACTGGCAGTCGACCGATGCCACACCTACCCTCACTAACTCCTCCTGAGCGGCCGC

Amino Acid Sequence

MDYKDDDDKGSPEFALSGGGPSGGGPGGSGRARTSSFAEPGGGGGGGGGG
PGGSASGPGGTGGGKASVGAMGGGVGASSSGGGPGGSGGGGSGGPGAGTS
FPPPGVKLGRDSGKVTTVIATLGQGPERSQEVAYTDIKVIGNGSFGVVYQ
ARLAETRELVAIKKVLQDKRFKNRELQIMRKLDHCNIVRLRYFFYSSGEK
KDELYLNLVLEYVPETVYRVARHFTKAKLTIPILYVKVYMYQLFRSLAYI
HSQGVCHRDIKPQNLLVDPDTAVLKLCDFGSAKQLVRGEPNVSYICSRYY
RAPELIFGATDYTSSIDVWSAGCVLAELLLGQPIFPGDSGVDQLVEIIKV
LGTPTREQIREMNPNYTEFKFPQIKAHPWTKVFKSRTPPEAIALCSSLLE
YTPSSRLSPLEACAHSFFDELRCLGTQLPNNRPLPPLFNFSAGELSIQPS
LNAILIPPHLRSPAGTTTLTPSSQALTETPTSSDWQSTDATPTLTNSS*

• Vector Type: Mammalian
• Antibiotic: Amp
• Full Snapgene Map: pCMV-FLAG-1 GSK3 alpha435.35 KBSnapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU35461
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter FLAG for mammalian expression; Variation: ; EST NCBI ACC. BC0227984; V104/I in this EST compared to the given Acc. Number; ; Estimated MW of expressed protein : 52689 Daltons
• Low Temp: No