cDNA Clone ATXN3

• Synonyms: AT3; JOS; MJD; ATX3; MJD1; SCA3
• Plasmid: pFastbacDual 6P1 ATXN3
• Parent Plasmid: pFastbacDual 6P1
• Expressed: GST 3C ATXN3
• Genbank: AAH33711.1
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGGAGTCCATCTTCCACGAGAAACAAGAAGGCTCACTTTGTGC
TCAACATTGCCTGAATAACTTATTGCAAGGAGAATATTTTAGCCCTGTGG
AATTATCCTCAATTGCACATCAGCTGGATGAGGAGGAGAGGATGAGAATG
GCAGAAGGAGGAGTTACTAGTGAAGATTATCGCACGTTTTTACAGCAGCC
TTCTGGAAATATGGATGACAGTGGTTTTTTCTCTATTCAGGTTATAAGCA
ATGCCTTGAAAGTTTGGGGTTTAGAACTAATCCTGTTCAACAGTCCAGAG
TATCAGAGGCTCAGGATCGATCCTATAAATGAAAGATCATTTATATGCAA
TTATAAGGAACACTGGTTTACAGTTAGAAAATTAGGAAAACAGTGGTTTA
ACTTGAATTCTCTCTTGACGGGTCCAGAATTAATATCAGATACATATCTT
GCACTTTTCTTGGCTCAATTACAACAGGAAGGTTATTCTATATTTGTCGT
TAAGGGTGATCTGCCAGATTGCGAAGCTGACCAACTCCTGCAGATGATTA
GGGTCCAACAGATGCATCGACCAAAACTTATTGGAGAAGAATTAGCACAA
CTAAAAGAGCAAAGAGTCCATAAAACAGACCTGGAACGAGTGTTAGAAGC
AAATGATGGCTCAGGAATGTTAGACGAAGATGAGGAGGATTTGCAGAGGG
CTCTGGCACTAAGTCGCCAAGAAATTGACATGGAAGATGAGGAAGCAGAT
CTCCGCAGGGCTATTCAGCTAAGTATGCAAGGTAGTTCCAGAAACATATC
TCAAGATATGACACAGACATCAGGTACAAATCTTACTTCAGAAGAGCTTC
GGAAGAGACGAGAAGCCTACTTTGAAAAACAGCAGCAAAAGCAGCAACAG
CAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGGG
GGACCTATCAGGACAGAGTTCACATCCATGTGAAAGGCCAGCCACCAGTT
CAGGAGCACTTGGGAGTGATCTAGGTGATGCTATGAGTGAAGAAGACATG
CTTCAGGCAGCTGTGACCATGTCTTTAGAAACTGTCAGAAATGATTTGAA
AACAGAAGGAAAAAAATAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMESIFHEKQEGSLCAQHCL
NNLLQGEYFSPVELSSIAHQLDEEERMRMAEGGVTSEDYRTFLQQPSGNM
DDSGFFSIQVISNALKVWGLELILFNSPEYQRLRIDPINERSFICNYKEH
WFTVRKLGKQWFNLNSLLTGPELISDTYLALFLAQLQQEGYSIFVVKGDL
PDCEADQLLQMIRVQQMHRPKLIGEELAQLKEQRVHKTDLERVLEANDGS
GMLDEDEEDLQRALALSRQEIDMEDEEADLRRAIQLSMQGSSRNISQDMT
QTSGTNLTSEELRKRREAYFEKQQQKQQQQQQQQQQQQQQQQQQQGDLSG
QSSHPCERPATSSGALGSDLGDAMSEEDMLQAAVTMSLETVRNDLKTEGK
K*

• Vector Type: Baculovirus
• Antibiotic: Amp
• Full Snapgene Map: pFastbacDual 6P1 ATXN364.47 KBSnapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU35451
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for baculovirus expression; ; Estimated MW of expressed protein : 69225 Daltons
• Low Temp: No