cDNA Clone MET

• Plasmid: pGEX6P-1-MET 956-1390
• Parent Plasmid: pGEX6P1
• Expressed: GST MET 956-1390
• Genbank: NP_000236.2
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCAAAAAGAGAAAGCAAATTAAAGATCTGGGCAGTGAATTAGTTCG
CTACGATGCAAGAGTACACACTCCTCATTTGGATAGGCTTGTAAGTGCCC
GAAGTGTAAGCCCAACTACAGAAATGGTTTCAAATGAATCTGTAGACTAC
CGAGCTACTTTTCCAGAAGATCAGTTTCCTAATTCATCTCAGAACGGTTC
ATGCCGACAAGTGCAGTATCCTCTGACAGACATGTCCCCCATCCTAACTA
GTGGGGACTCTGATATATCCAGTCCATTACTGCAAAATACTGTCCACATT
GACCTCAGTGCTCTAAATCCAGAGCTGGTCCAGGCAGTGCAGCATGTAGT
GATTGGGCCCAGTAGCCTGATTGTGCATTTCAATGAAGTCATAGGAAGAG
GGCATTTTGGTTGTGTATATCATGGGACTTTGTTGGACAATGATGGCAAG
AAAATTCACTGTGCTGTGAAATCCTTGAACAGAATCACTGACATAGGAGA
AGTTTCCCAATTTCTGACCGAGGGAATCATCATGAAAGATTTTAGTCATC
CCAATGTCCTCTCGCTCCTGGGAATCTGCCTGCGAAGTGAAGGGTCTCCG
CTGGTGGTCCTACCATACATGAAACATGGAGATCTTCGAAATTTCATTCG
AAATGAGACTCATAATCCAACTGTAAAAGATCTTATTGGCTTTGGTCTTC
AAGTAGCCAAAGGCATGAAATATCTTGCAAGCAAAAAGTTTGTCCACAGA
GACTTGGCTGCAAGAAACTGTATGCTGGATGAAAAATTCACAGTCAAGGT
TGCTGATTTTGGTCTTGCCAGAGACATGTATGATAAAGAATACTATAGTG
TACACAACAAAACAGGTGCAAAGCTGCCAGTGAAGTGGATGGCTTTGGAA
AGTCTGCAAACTCAAAAGTTTACCACCAAGTCAGATGTGTGGTCCTTTGG
CGTGCTCCTCTGGGAGCTGATGACAAGAGGAGCCCCACCTTATCCTGACG
TAAACACCTTTGATATAACTGTTTACTTGTTGCAAGGGAGAAGACTCCTA
CAACCCGAATACTGCCCAGACCCCTTATATGAAGTAATGCTAAAATGCTG
GCACCCTAAAGCCGAAATGCGCCCATCCTTTTCTGAACTGGTGTCCCGGA
TATCAGCGATCTTCTCTACTTTCATTGGGGAGCACTATGTCCATGTGAAC
GCTACTTATGTGAACGTAAAATGTGTCGCTCCGTATCCTTCTCTGTTGTC
ATCAGAAGATAACGCTGATGATGAGGTGGACACACGACCAGCCTCCTTCT
GGGAGACATCATAGGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSKKRKQIKDLGSELVRYDAR
VHTPHLDRLVSARSVSPTTEMVSNESVDYRATFPEDQFPNSSQNGSCRQV
QYPLTDMSPILTSGDSDISSPLLQNTVHIDLSALNPELVQAVQHVVIGPS
SLIVHFNEVIGRGHFGCVYHGTLLDNDGKKIHCAVKSLNRITDIGEVSQF
LTEGIIMKDFSHPNVLSLLGICLRSEGSPLVVLPYMKHGDLRNFIRNETH
NPTVKDLIGFGLQVAKGMKYLASKKFVHRDLAARNCMLDEKFTVKVADFG
LARDMYDKEYYSVHNKTGAKLPVKWMALESLQTQKFTTKSDVWSFGVLLW
ELMTRGAPPYPDVNTFDITVYLLQGRRLLQPEYCPDPLYEVMLKCWHPKA
EMRPSFSELVSRISAIFSTFIGEHYVHVNATYVNVKCVAPYPSLLSSEDN
ADDEVDTRPASFWETS*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU35360
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for bacterial expression; ; Estimated MW of expressed protein : 75852 Daltons