cDNA Clone PCTK1

• Plasmid: pGEX6P-1-PCTK1 165-446
• Parent Plasmid: pGEX6P1
• Expressed: GST PCTK1 165-446
• Genbank: NM_006201
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCTACATTAAGCTGGACAAACTGGGCGAGGGTACCTATGCCACCGT
CTACAAAGGCAAAAGCAAGCTCACAGACAACCTTGTGGCACTCAAGGAGA
TCAGACTGGAACATGAAGAGGGGGCACCCTGCACCGCCATCCGGGAAGTG
TCCCTGCTCAAGGACCTCAAACACGCCAACATCGTTACGCTACATGACAT
TATCCACACGGAGAAGTCCCTCACCCTTGTCTTTGAGTACCTGGACAAGG
ACCTGAAGCAGTACCTGGATGACTGTGGGAACATCATCAACATGCACAAC
GTGAAACTGTTCCTGTTCCAGCTGCTCCGTGGCCTGGCCTACTGCCACCG
GCAGAAGGTGCTACACCGAGACCTCAAGCCCCAGAACCTGCTCATCAACG
AGAGGGGAGAGCTCAAGCTGGCTGACTTTGGCCTGGCCCGAGCCAAGTCA
ATCCCAACAAAGACATACTCCAATGAGGTGGTGACACTGTGGTACCGGCC
CCCTGACATCCTGCTTGGGTCCACGGACTACTCCACTCAGATTGACATGT
GGGGTGTGGGCTGCATCTTCTATGAGATGGCCACAGGCCGTCCCCTCTTT
CCGGGCTCCACGGTGGAGGAACAGCTACACTTCATCTTCCGTATCTTAGG
AACCCCAACTGAGGAGACGTGGCCAGGCATCCTGTCCAACGAGGAGTTCA
AGACATACAACTACCCCAAGTACCGAGCCGAGGCCCTTTTGAGCCACGCA
CCCCGACTTGATAGCGACGGGGCCGACCTCCTCACCAAGCTGTTGCAGTT
TGAGGGTCGAAATCGGATCTCCGCAGAGGATGCCATGAAACATCCATTCT
TCTAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSYIKLDKLGEGTYATVYKGK
SKLTDNLVALKEIRLEHEEGAPCTAIREVSLLKDLKHANIVTLHDIIHTE
KSLTLVFEYLDKDLKQYLDDCGNIINMHNVKLFLFQLLRGLAYCHRQKVL
HRDLKPQNLLINERGELKLADFGLARAKSIPTKTYSNEVVTLWYRPPDIL
LGSTDYSTQIDMWGVGCIFYEMATGRPLFPGSTVEEQLHFIFRILGTPTE
ETWPGILSNEEFKTYNYPKYRAEALLSHAPRLDSDGADLLTKLLQFEGRN
RISAEDAMKHPFF*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU35359
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for bacterial expression; ; Estimated MW of expressed protein : 59185 Daltons