cDNA Clone PTEN

• Plasmid: pEGFP-PTEN Y138L
• Parent Plasmid: pEGFPC2
• Expressed: GFP PTEN Y138L
• Genbank: CR450306
• Tag: GFP
• Species: Human

Sequence of Insert

gaattcGCCCTTCTCCCAGACATGACAGCCATCATCAAAGAGATCGTTAG
CAGAAACAAAAGGAGATATCAAGAGGATGGATTCGACTTAGACTTGACCT
ATATTTATCCAAACATTATTGCTATGGGATTTCCTGCAGAAAGACTTGAA
GGCGTATACAGGAACAATATTGATGATGTAGTAAGGTTTTTGGATTCAAA
GCATAAAAACCATTACAAGATATACAATCTTTGTGCTGAAAGACATTATG
ACACCGCCAAATTTAATTGCAGAGTTGCACAATATCCTTTTGAAGACCAT
AACCCACCACAGCTAGAACTTATCAAACCCTTTTGTGAAGATCTTGACCA
ATGGCTAAGTGAAGATGACAATCATGTTGCAGCAATTCACTGTAAAGCTG
GAAAGGGACGAACTGGTGTAATGATATGTGCATTGTTATTACATCGGGGC
AAATTTTTAAAGGCACAAGAGGCCCTAGATTTCTATGGGGAAGTAAGGAC
CAGAGACAAAAAGGGAGTAACTATTCCCAGTCAGAGGCGCTATGTGTATT
ATTATAGCTACCTGTTAAAGAATCATCTGGATTATAGACCAGTGGCACTG
TTGTTTCACAAGATGATGTTTGAAACTATTCCAATGTTCAGTGGCGGAAC
TTGCAATCCTCAGTTTGTGGTCTGCCAGCTAAAGGTGAAGATATATACCT
CCAATTCAGGACCCACACGACGGGAAGACAAGTTCATGTACTTTGAGTTC
CCTCAGCCGTTACCTGTGTGTGGTGATATCAAAGTAGAGTTCTTCCACAA
ACAGAACAAGATGCTAAAAAAGGACAAAATGTTTCACTTTTGGGTAAATA
CATTCTTCATACCAGGACCAGAGGAAACCTCAGAAAAAGTAGAAAATGGA
AGTCTATGTGATCAAGAAATCGATAGCATTTGCAGTATAGAGCGTGCAGA
TAATGACAAGGAATATCTAGTACTTACTTTAACAAAAAATGATCTTGACA
AAGCAAATAAAGACAAAGCCAACCGATACTTTTCTCCAAATTTTAAGGTG
AAGCTGTACTTCACAAAAACAGTAGAGGAGCCGTCAAATCCAGAGGCTAG
CAGTTCAACTTCTGTAACACCAGATGTTAGTGACAATGAACCTGATCATT
ATAGATATTCTGACACCACTGACTCTGATCCAGAGAATGAACCTTTTGAT
GAAGATCAGCATACACAAATTACAAAAGTCTGAgaattc

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGRTQISSSSF
EFALLPDMTAIIKEIVSRNKRRYQEDGFDLDLTYIYPNIIAMGFPAERLE
GVYRNNIDDVVRFLDSKHKNHYKIYNLCAERHYDTAKFNCRVAQYPFEDH
NPPQLELIKPFCEDLDQWLSEDDNHVAAIHCKAGKGRTGVMICALLLHRG
KFLKAQEALDFYGEVRTRDKKGVTIPSQRRYVYYYSYLLKNHLDYRPVAL
LFHKMMFETIPMFSGGTCNPQFVVCQLKVKIYSSNSGPTRREDKFMYFEF
PQPLPVCGDIKVEFFHKQNKMLKKDKMFHFWVNTFFIPGPEETSEKVENG
SLCDQEIDSICSIERADNDKEYLVLTLTKNDLDKANKDKANRYFSPNFKV
KLYFTKTVEEPSNPEASSSTSVTPDVSDNEPDHYRYSDTTDSDPENEPFD
EDQHTQITKV*

• Vector Type: Mammalian
• Antibiotic: Kan
• DU Number: DU7165
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Estimated MW of expressed protein : 75962 Daltons