cDNA Clone Abl1 (mouse)

• Plasmid: pcDNA3.1 hygro GST Abl1 (mouse)
• Parent Plasmid: pcDNA3.1 hygro GST
• Expressed: GST Abl1 (mouse)
• Genbank: AAH59260.1
• Tag: GST
• Species: Mouse

Sequence of Insert

GGATCCATGGGGCAGCAGCCTGGAAAAGTTCTTGGGGACCAAAGAAGGCC
TAGTTTGCCCGCCCTGCATTTTATCAAAGGGGCAGGGAAGAGGGACTCAT
CGAGGCATGGGGGCCCACACTGCAATGTCTTTGTGGAACACGAAGCCCTG
CAGAGGCCAGTGGCATCTGACTTTGAGCCCCAGGGTCTCAGCGAAGCAGC
TCGATGGAACTCCAAGGAAAACCTTCTTGCTGGGCCCAGTGAAAATGACC
CCAACCTTTTTGTGGCACTCTATGATTTTGTGGCCAGTGGAGATAACACT
CTCAGCATCACTAAAGGTGAAAAGCTCCGGGTCTTGGGTTATAATCACAA
TGGGGAATGGTGTGAAGCCCAAACGAAAAATGGCCAAGGATGGGTCCCAA
GCAACTACATCACCCCCGTCAACAGCCTGGAGAAACATTCCTGGTATCAT
GGCCCTGTATCTCGGAATGCTGCTGAGTATCTGCTGAGCAGCGGAATCAA
CGGCAGCTTCTTAGTGCGGGAGAGTGAGAGTAGCCCTGGCCAGAGATCCA
TCTCGCTGCGGTATGAAGGGAGGGTGTACCACTACAGGATCAACACTGCC
TCTGATGGCAAGCTGTACGTGTCCTCCGAGAGCCGCTTCAACACTCTGGC
TGAGTTAGTTCACCATCACTCCACGGTGGCTGATGGCCTCATCACCACAC
TCCACTACCCAGCTCCCAAGCGCAACAAGCCCACTATCTACGGTGTGTCC
CCCAACTACGACAAGTGGGAAATGGAGCGCACCGACATCACCATGAAGCA
CAAGTTGGGTGGAGGCCAGTACGGGGAGGTGTACGAGGGCGTTTGGAAGA
AGTACAGCCTCACTGTGGCCGTGAAGACCTTGAAGGAGGACACCATGGAG
GTGGAGGAGTTCCTGAAGGAAGCGGCGGTGATGAAGGAGATCAAACACCC
TAACCTGGTGCAGCTGCTAGGGGTGTGTACCCGGGAACCACCATTCTACA
TAATCACTGAGTTCATGACCTATGGGAACCTGCTGGACTACCTGAGGGAG
TGTAACCGGCAGGAGGTGAGCGCCGTGGTACTGCTCTACATGGCCACACA
GATCTCATCAGCCATGGAGTACTTGGAGAAGAAGAACTTCATCCACAGAG
ACCTTGCTGCCCGGAACTGCCTGGTAGGGGAAAACCACTTGGTGAAGGTG
GCTGATTTTGGCCTGAGCAGGTTGATGACAGGGGACACCTACACGGCCCA
TGCTGGAGCCAAATTCCCCATCAAATGGACCGCACCTGAGAGCCTGGCCT
ACAACAAGTTCTCCATCAAGTCGGACGTGTGGGCATTTGGAGTATTGCTC
TGGGAGATTGCTACCTATGGCATGTCACCTTACCCGGGAATTGACCTGTC
TCAGGTTTATGAGCTGCTGGAAAAAGACTACCGCATGGAGCGCCCTGAAG
GCTGCCCGGAGAAGGTCTACGAGCTCATGCGAGCATGTTGGCAGTGGAAC
CCCTCTGACCGGCCCTCCTTTGCTGAAATCCACCAAGCCTTTGAAACCAT
GTTCCAGGAATCCAGTATCTCAGATGAGGTGGAGAAGGAGCTGGGGAAAC
GAGGCACGAGAGGAGGTGCTGGGAGTATGCTGCAGGCCCCAGAGCTGCCC
ACCAAGACCAGAACCTGCAGGAGAGCAGCTGAGCAGAAAGATGCGCCTGA
CACCCCTGAGCTGCTCCACACGAAGGGCCTGGGAGAAAGCGATGCACTGG
ACAGTGAGCCTGCTGTATCGCCACTGCTTCCTCGGAAAGAGCGCGGGCCC
CCAGACGGCAGCCTAAATGAAGATGAGCGCCTTCTCCCCAGAGACAGAAA
GACCAACCTGTTCAGCGCTTTGATCAAGAAGAAGAAGAAAATGGCGCCGA
CGCCCCCTAAGCGCAGCAGTTCCTTCCGAGAGATGGATGGCCAGCCAGAC
CGCAGAGGGGCTAGTGAGGATGACAGCAGGGAACTCTGCAATGGACCACC
AGCTCTCACCTCAGACGCAGCAGAGCCTACCAAGTCCCCAAAGGCCAGCA
ATGGGGCTGGCGTCCCTAATGGAGCCTTCCGGGAGCCGGGCAACTCAGGC
TTCCGTTCTCCCCACATGTGGAAAAAGTCCAGCACACTGACCGGGAGCCG
CCTGGCTGCTGCCGAAGAGGAGAGCGGCATGAGCTCCAGTAAGCGCTTCC
TGCGTTCTTGTTCGGCCTCCTGCATGCCCCATGGGGCAAGGGACACAGAG
TGGCGGTCGGTCACGCTGCCTCGAGACCTGCCGTCTGCTGGCAAGCAGTT
TGACTCATCCACCTTTGGAGGGCACAAAAGCGAAAAGCCAGCTCTGCCTC
GGAAACGCACCAGTGAGAGCAGGTCTGAGCAGGTGGCCAAAAGCACGGCG
ATGCCCCCTCCCCGGCTGGTGAAGAAGAACGAGGAGGCTGCTGAAGAAGG
CTTCAAAGACACAGAATCCAGCCCTGGCTCCAGCCCTCCCAGCTTGACTC
CCAAACTCCTCCGCAGGCAGGTCACTGCCTCTCCTTCCTCTGGCCTCTCT
CACAAGGAAGAGGCCACCAAGGGCAGTGCCTCAGGCATGGGGACTCCGGC
CACTGCAGAGCCAGCACCCCCCAGCAACAAAGTGGGCCTCAGCAAGGCCT
CCTCTGAGGAGATGCGCGTAAGGAGGCACAAGCACAGCTCGGAGTCCCCA
GGGAGAGACAAGGGGCGACTGGCTAAGCTCAAGCCTGCCCCGCCGCCTCC
TCCTGCCTGCACAGGAAAAGCAGGCAAGCCCGCACAGAGCCCCAGCCAAG
AGGCCGGGGAGGCAGGGGGGCCCACAAAGACAAAATGCACGAGTCTGGCT
ATGGATGCTGTGAACACTGACCCCACCAAGGCCGGCCCACCTGGAGAAGG
ACTGAGAAAGCCTGTGCCCCCATCTGTGCCAAAGCCCCAGTCGACGGCTA
AGCCTCCAGGGACTCCCACCAGCCCGGTCTCCACCCCCTCCACAGCACCA
GCTCCTTCACCCCTGGCTGGGGACCAGCAGCCATCTTCTGCCGCCTTCAT
CCCCCTCATATCAACCCGTGTGTCTCTTAGGAAGACCCGCCAGCCGCCAG
AGCGCATTGCCAGTGGCACCATCACCAAGGGTGTGGTTCTGGACAGTACT
GAGGCCCTGTGCCTTGCCATCTCCCGGAACTCAGAGCAGATGGCCAGCCA
CAGTGCTGTACTGGAGGCTGGCAAGAACCTGTACACTTTCTGTGTGAGCT
ATGTGGACTCTATCCAGCAGATGAGGAACAAGTTTGCCTTCCGTGAGGCT
ATCAACAAGCTGGAGAGCAACCTCCGAGAGCTGCAGATCTGCCCTGCCAC
AGCCTCCAGTGGGCCAGCTGCCACCCAAGACTTCAGCAAGCTGCTCAGCT
CTGTGAAGGAGATCAGCGACATTGTCCGGAGGTAGGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMGQQPGKVLGDQRRPSLPA
LHFIKGAGKRDSSRHGGPHCNVFVEHEALQRPVASDFEPQGLSEAARWNS
KENLLAGPSENDPNLFVALYDFVASGDNTLSITKGEKLRVLGYNHNGEWC
EAQTKNGQGWVPSNYITPVNSLEKHSWYHGPVSRNAAEYLLSSGINGSFL
VRESESSPGQRSISLRYEGRVYHYRINTASDGKLYVSSESRFNTLAELVH
HHSTVADGLITTLHYPAPKRNKPTIYGVSPNYDKWEMERTDITMKHKLGG
GQYGEVYEGVWKKYSLTVAVKTLKEDTMEVEEFLKEAAVMKEIKHPNLVQ
LLGVCTREPPFYIITEFMTYGNLLDYLRECNRQEVSAVVLLYMATQISSA
MEYLEKKNFIHRDLAARNCLVGENHLVKVADFGLSRLMTGDTYTAHAGAK
FPIKWTAPESLAYNKFSIKSDVWAFGVLLWEIATYGMSPYPGIDLSQVYE
LLEKDYRMERPEGCPEKVYELMRACWQWNPSDRPSFAEIHQAFETMFQES
SISDEVEKELGKRGTRGGAGSMLQAPELPTKTRTCRRAAEQKDAPDTPEL
LHTKGLGESDALDSEPAVSPLLPRKERGPPDGSLNEDERLLPRDRKTNLF
SALIKKKKKMAPTPPKRSSSFREMDGQPDRRGASEDDSRELCNGPPALTS
DAAEPTKSPKASNGAGVPNGAFREPGNSGFRSPHMWKKSSTLTGSRLAAA
EEESGMSSSKRFLRSCSASCMPHGARDTEWRSVTLPRDLPSAGKQFDSST
FGGHKSEKPALPRKRTSESRSEQVAKSTAMPPPRLVKKNEEAAEEGFKDT
ESSPGSSPPSLTPKLLRRQVTASPSSGLSHKEEATKGSASGMGTPATAEP
APPSNKVGLSKASSEEMRVRRHKHSSESPGRDKGRLAKLKPAPPPPPACT
GKAGKPAQSPSQEAGEAGGPTKTKCTSLAMDAVNTDPTKAGPPGEGLRKP
VPPSVPKPQSTAKPPGTPTSPVSTPSTAPAPSPLAGDQQPSSAAFIPLIS
TRVSLRKTRQPPERIASGTITKGVVLDSTEALCLAISRNSEQMASHSAVL
EAGKNLYTFCVSYVDSIQQMRNKFAFREAINKLESNLRELQICPATASSG
PAATQDFSKLLSSVKEISDIVRR*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU35108
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian N ter GST tag with hygromycin selection; ; Estimated MW of expressed protein : 151586 Daltons