cDNA Clone VIP2

• Plasmid: pFBDual6P-1 VIP2
• Parent Plasmid: pFastbacDual 6P1
• Expressed: GST VIP2
• Genbank: NP_056031.2
• Tag: GST
• Species: Human

Sequence of Insert

GAATTCATGAGTGAAGCCCCCAGATTCTTCGTTGGACCAGAAGATACAGA
AATAAATCCTGGAAATTATCGACATTTCTTCCACCATGCAGATGAAGACG
ATGAGGAGGAAGATGATTCTCCACCAGAAAGGCAGATTGTGGTTGGAATA
TGTTCCATGGCAAAGAAATCCAAATCCAAACCAATGAAGGAAATTCTTGA
ACGGATCTCCTTATTTAAATATATCACAGTAGTAGTATTTGAAGAGGAGG
TTATTTTGAATGAACCAGTGGAAAACTGGCCTTTATGTGATTGTCTTATT
TCTTTCCATTCTAAAGGATTTCCACTGGACAAAGCGGTTGCCTATGCAAA
ACTCAGGAATCCATTTGTAATCAATGACTTGAATATGCAGTATCTCATAC
AAGATAGGAGAGAAGTATATAGTATTCTTCAAGCTGAAGGTATTTTACTT
CCTCGTTATGCTATTTTGAACCGTGACCCAAATAATCCCAAAGAATGTAA
TCTGATTGAAGGGGAAGATCATGTAGAAGTAAATGGGGAAGTTTTTCAAA
AGCCATTTGTAGAAAAGCCAGTCAGTGCAGAAGATCACAATGTTTACATT
TATTACCCAACTTCTGCTGGTGGTGGAAGTCAAAGACTCTTTAGAAAGAT
TGGCAGTAGAAGTAGTGTTTATTCTCCAGAAAGCAATGTACGAAAAACAG
GCTCATATATATATGAAGAGTTTATGCCCACAGATGGTACTGATGTTAAG
GTTTATACAGTGGGTCCAGATTATGCCCATGCTGAAGCTCGAAAATCTCC
AGCACTTGATGGCAAGGTGGAACGAGACAGTGAAGGAAAAGAAGTAAGAT
ACCCTGTTATTCTCAATGCACGAGAGAAATTAATTGCTTGGAAAGTCTGC
CTTGCTTTTAAGCAAACAGTTTGTGGCTTTGATTTGTTACGGGCCAATGG
ACAGTCCTATGTCTGTGATGTCAATGGCTTCAGTTTTGTGAAAAATTCCA
TGAAGTATTATGATGACTGTGCAAAAATACTTGGAAATATTGTAATGCGA
GAACTTGCTCCACAATTTCATATTCCATGGTCAATACCCTTAGAAGCTGA
AGATATCCCAATTGTACCAACTACATCTGGAACTATGATGGAACTTAGAT
GTGTCATAGCTGTTATACGTCATGGGGATCGAACACCAAAACAAAAAATG
AAAATGGAAGTGAGACATCAGAAATTTTTTGATCTTTTTGAAAAGTGTGA
TGGATATAAATCAGGGAAATTAAAACTCAAAAAACCAAAACAGTTACAGG
AAGTGCTAGATATTGCACGACAGCTTCTTATGGAGCTAGGGCAAAATAAT
GATTCTGAAATTGAAGAAAACAAGCCAAAACTTGAACAACTTAAGACTGT
ATTAGAGATGTATGGTCATTTTTCTGGAATAAATCGTAAGGTTCAGTTGA
CCTATCTCCCTCATGGTTGTCCTAAAACATCTAGTGAAGAGGAGGACAGC
CGAAGAGAAGAACCATCTTTACTTTTGGTTCTAAAATGGGGAGGTGAATT
AACTCCTGCAGGCAGGGTCCAGGCTGAAGAACTTGGAAGAGCCTTCAGGT
GTATGTATCCTGGAGGTCAAGGAGATTATGCAGGATTTCCTGGTTGTGGT
TTACTTAGATTACATAGCACCTACAGACATGACCTCAAAATATATGCCTC
TGATGAAGGACGAGTCCAGATGACTGCAGCTGCTTTTGCAAAGGGGCTTT
TAGCTTTGGAAGGAGAGCTTACACCCATTCTTGTTCAAATGGTGAAAAGT
GCAAATATGAACGGTCTTTTGGATAGTGATAGTGACTCTCTGAGCAGTTG
TCAGCAACGTGTGAAGGCAAGGCTTCATGAAATACTTCAGAAAGACAGAG
ATTTTACTGCTGAAGATTATGAAAAGCTTACTCCATCTGGAAGCATTTCT
CTTATCAAATCAATGCATTTAATTAAAAACCCTGTGAAGACCTGTGATAA
AGTTTATTCCTTAATTCAGAGTTTGACTTCTCAAATCAGACATCGAATGG
AAGATCCTAAATCATCAGATATTCAGCTTTACCATAGTGAAACATTGGAG
CTTATGCTACGTAGATGGTCCAAGTTAGAGAAAGACTTTAAAACAAAGAA
TGGAAGATATGATATTAGTAAAATCCCTGACATATATGACTGTATAAAAT
ATGATGTCCAGCATAATGGTTCCTTGAAATTAGAAAACACAATGGAATTA
TATAGGCTTTCGAAGGCATTAGCAGATATTGTTATCCCTCAGGAATATGG
TATAACTAAAGCTGAAAAACTGGAGATTGCCAAAGGCTACTGTACTCCTC
TGGTTAGAAAAATTCGCTCAGACCTTCAGAGGACACAAGATGATGACACT
GTAAATAAACTTCATCCTGTGTATTCTAGAGGTGTTCTGTCTCCTGAACG
TCATGTTCGTACTAGATTATATTTTACCAGTGAAAGTCATGTACATTCTT
TGCTGTCTATTCTTCGCTATGGTGCCTTATGCAATGAATCAAAGGATGAA
CAGTGGAAACGAGCTATGGATTATTTAAACGTTGTCAATGAGCTCAACTA
CATGACTCAGATTGTTATCATGCTTTATGAGGATCCTAATAAGGATCTTT
CCTCAGAAGAACGCTTTCATGTTGAATTACACTTTAGTCCGGGAGCCAAA
GGTTGTGAAGAAGACAAAAATTTGCCATCTGGCTATGGATATAGACCAGC
TTCCAGAGAGAATGAAGGCAGGAGACCTTTTAAAATTGATAATGATGATG
AACCACATACTTCTAAAAGAGATGAAGTTGATCGAGCTGTGATATTGTTT
AAACCAATGGTATCAGAGCCAATTCATATACACAGGAAGTCTCCACTTCC
AAGATCTAGGAAGACGGCTACAAATGATGAAGAGAGCCCCCTGAGTGTGT
CTAGCCCAGAGGGTACTGGTACCTGGCTGCATTATACCAGTGGTGTGGGT
ACTGGGCGTCGAAGACGCAGATCAGGGGAACAAATCACTTCTTCCCCTGT
CTCCCCCAAATCATTGGCTTTCACATCCAGTATTTTTGGCTCATGGCAAC
AGGTTGTATCTGAAAATGCTAATTACCTGCGAACACCAAGAACTCTTGTG
GAACAGAAGCAGAATCCTACTGTAGGGTCTCACTGTGCGGGCCTGTTTAG
CACCTCGGTGCTCGGGGGTTCTTCAAGCGCACCTAACCTACAGGATTATG
CTCGTACTCATCGTAAAAAGCTGACCTCTTCTGGCTGCATAGATGGCTTT
GAATTGTATTCCATGGTGCCATCTATTTGTCCTCTAGAAACTCTTCATAA
TGCCCTATCTTTAAAGCAAGTGGATGAATTTCTTGCTTCCATTGCTTCTC
CATCATCTGACGTTCCACGGAAGACCGCTGAAATTTCCTCTACAGCTTTA
CGTTCCAGTCCAATAATGAGAAAAAAAGTATCTTTAAATACGTATACACC
TGCAAAGATCCTCCCAACACCACCAGCTACCTTAAAGAGCACTAAAGCAA
GCAGCAAACCAGCTACAAGTGGACCTTCTAGTGCAGTTGTTCCTAATACC
TCATCTCGGAAAAAGAATATAACTAGCAAAACAGAAACGCATGAACACAA
AAAAAACACTGGGAAAAAGAAATGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPEFMSEAPRFFVGPEDTEI
NPGNYRHFFHHADEDDEEEDDSPPERQIVVGICSMAKKSKSKPMKEILER
ISLFKYITVVVFEEEVILNEPVENWPLCDCLISFHSKGFPLDKAVAYAKL
RNPFVINDLNMQYLIQDRREVYSILQAEGILLPRYAILNRDPNNPKECNL
IEGEDHVEVNGEVFQKPFVEKPVSAEDHNVYIYYPTSAGGGSQRLFRKIG
SRSSVYSPESNVRKTGSYIYEEFMPTDGTDVKVYTVGPDYAHAEARKSPA
LDGKVERDSEGKEVRYPVILNAREKLIAWKVCLAFKQTVCGFDLLRANGQ
SYVCDVNGFSFVKNSMKYYDDCAKILGNIVMRELAPQFHIPWSIPLEAED
IPIVPTTSGTMMELRCVIAVIRHGDRTPKQKMKMEVRHQKFFDLFEKCDG
YKSGKLKLKKPKQLQEVLDIARQLLMELGQNNDSEIEENKPKLEQLKTVL
EMYGHFSGINRKVQLTYLPHGCPKTSSEEEDSRREEPSLLLVLKWGGELT
PAGRVQAEELGRAFRCMYPGGQGDYAGFPGCGLLRLHSTYRHDLKIYASD
EGRVQMTAAAFAKGLLALEGELTPILVQMVKSANMNGLLDSDSDSLSSCQ
QRVKARLHEILQKDRDFTAEDYEKLTPSGSISLIKSMHLIKNPVKTCDKV
YSLIQSLTSQIRHRMEDPKSSDIQLYHSETLELMLRRWSKLEKDFKTKNG
RYDISKIPDIYDCIKYDVQHNGSLKLENTMELYRLSKALADIVIPQEYGI
TKAEKLEIAKGYCTPLVRKIRSDLQRTQDDDTVNKLHPVYSRGVLSPERH
VRTRLYFTSESHVHSLLSILRYGALCNESKDEQWKRAMDYLNVVNELNYM
TQIVIMLYEDPNKDLSSEERFHVELHFSPGAKGCEEDKNLPSGYGYRPAS
RENEGRRPFKIDNDDEPHTSKRDEVDRAVILFKPMVSEPIHIHRKSPLPR
SRKTATNDEESPLSVSSPEGTGTWLHYTSGVGTGRRRRRSGEQITSSPVS
PKSLAFTSSIFGSWQQVVSENANYLRTPRTLVEQKQNPTVGSHCAGLFST
SVLGGSSSAPNLQDYARTHRKKLTSSGCIDGFELYSMVPSICPLETLHNA
LSLKQVDEFLASIASPSSDVPRKTAEISSTALRSSPIMRKKVSLNTYTPA
KILPTPPATLKSTKASSKPATSGPSSAVVPNTSSRKKNITSKTETHEHKK
NTGKKK

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU32983
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for baculovirus expression; ; Estimated MW of expressed protein : 165299 Daltons