cDNA Clone B galactosidase

• Synonyms: GLB1
• Plasmid: pGEX6P-1-beta gal 3-94 C76S C tail
• Parent Plasmid: pGEX6P1
• Expressed: GST beta gal 3-94 C76S C tail
• Genbank: BAD83864
• Tag: GST

Sequence of Insert

GGATCCATTACGGATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGA
AAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCG
CCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAG
TTGCGCAGCCTGAATGGCGAATGGCGCTTTGCCTGGTTTCCGGCACCAGA
AGCGGTGCCGGAAAGCTGGCTGGAGTCCGATCTTCCTGAGGCCGATACTG
TCGTCGTCCCCTCAAACTGGCAGATGGGTTCTCAACATTGTCATCAATTT
CGTTTTCGTGGTCGTTCTCGTGCTGCTTGTGGTTAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSITDSLAVVLQRRDWENPGV
TQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRFAWFPAPEAVPE
SWLESDLPEADTVVVPSNWQMGSQHCHQFRFRGRSRAACG*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU32932
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for bacterial expression