cDNA Clone HPK1

• Synonyms: MAP4K1
• Plasmid: pGEX6P-2-HPK1
• Parent Plasmid: pGEX6P2
• Expressed: GST HPK1
• Genbank: NP_001036065.1
• Tag: GST
• Species: Human

Sequence of Insert

GCGGCCGCGATGGACGTCGTGGACCCTGACATTTTCAATAGAGACCCCCG
GGACCACTATGACCTGCTACAGCGGCTGGGTGGCGGCACGTATGGGGAAG
TCTTTAAGGCTCGAGACAAGGTGTCAGGGGACCTGGTGGCACTGAAGATG
GTGAAGATGGAGCCTGATGATGATGTCTCCACCCTTCAGAAGGAAATCCT
CATATTGAAAACTTGCCGGCACGCCAACATCGTGGCCTACCATGGGAGTT
ATCTCTGGTTGCAGAAACTCTGGATCTGCATGGAATTCTGTGGGGCTGGT
TCTCTCCAGGACATCTACCAAGTGACAGGCTCCCTGTCAGAGCTCCAGAT
TAGCTATGTCTGCCGGGAAGTGCTCCAGGGACTGGCCTATTTGCACTCAC
AGAAGAAGATACACAGGGACATCAAGGGAGCTAACATCCTCATCAATGAT
GCTGGGGAGGTCAGATTGGCTGACTTTGGCATCTCGGCCCAGATTGGGGC
TACACTGGCCAGACGCCTCTCTTTCATTGGGACACCCTACTGGATGGCTC
CGGAAGTGGCAGCTGTGGCCCTGAAGGGAGGATACAATGAGCTGTGTGAC
ATCTGGTCCCTGGGCATCACGGCCATCGAACTGGCCGAGCTACAGCCACC
GCTCTTTGATGTGCACCCTCTCAGAGTTCTCTTCCTCATGACCAAGAGTG
GCTACCAGCCTCCCCGACTGAAGGAAAAAGGCAAATGGTCGGCTGCCTTC
CACAACTTCATCAAAGTCACTCTGACTAAGAGTCCCAAGAAACGACCCAG
CGCCACCAAGATGCTCAGTCATCAACTGGTATCCCAGCCTGGGCTGAATC
GAGGCCTGATCCTGGATCTTCTTGACAAACTGAAGAATCCCGGGAAAGGA
CCCTCCATTGGGGACATTGAGGATGAGGAGCCCGAGCTACCCCCTGCTAT
CCCTCGGCGGATCAGATCCACCCACCGCTCCAGCTCTCTGGGGATCCCAG
ATGCAGACTGCTGTCGGCGGCACATGGAGTTCAGGAAGCTCCGAGGAATG
GAGACCAGACCCCCAGCCAACACCGCTCGCCTACAGCCTCCTCGAGACCT
CAGGAGCAGCAGCCCCAGGAAGCAACTGTCAGAGTCGTCTGACGATGACT
ATGACGACGTGGACATCCCCACCCCTGCAGAGGACACACCTCCTCCACTT
CCCCCCAAGCCCAAGTTCCGTTCTCCATCAGACGAGGGTCCTGGGAGCAT
GGGGGATGATGGGCAGCTGAGCCCGGGGGTGCTGGTCCGGTGTGCCAGTG
GGCCCCCACCAAACAGCCCCCGTCCTGGGCCTCCCCCATCCACCAGCAGC
CCCCACCTCACCGCCCATTCAGAACCCTCACTCTGGAACCCACCCTCCCG
GGAGCTTGACAAGCCCCCACTTCTGCCCCCCAAGAAGGAAAAGATGAAGA
GAAAGGGATGTGCCCTTCTCGTAAAGTTGTTCAATGGCTGCCCCCTCCGG
ATCCACAGCACGGCCGCCTGGACACATCCCTCCACCAAGGACCAGCACCT
GCTCCTGGGGGCAGAGGAAGGCATCTTTATCCTGAACCGGAATGACCAGG
AGGCCACGCTGGAAATGCTCTTTCCTAGCCGGACTACGTGGGTGTACTCC
ATCAACAACGTTCTCATGTCTCTCTCAGGAAAGACCCCCCACCTGTATTC
TCATAGCATCCTTGGCCTGCTGGAACGGAAAGAGACCAGAGCAGGAAACC
CCATCGCTCACATTAGCCCCCACCGCCTACTGGCAAGGAAGAACATGGTT
TCCACCAAGATCCAGGACACCAAAGGCTGCCGGGCGTGCTGTGTGGCGGA
GGGTGCGAGCTCTGGGGGCCCGTTCCTGTGCGGTGCATTGGAGACGTCCG
TTGTCCTGCTTCAGTGGTACCAGCCCATGAACAAATTCCTGCTTGTCCGG
CAGGTGCTGTTCCCACTGCCGACGCCTCTGTCCGTGTTCGCGCTGCTGAC
CGGGCCAGGCTCTGAGCTGCCCGCTGTGTGCATCGGCGTGAGCCCCGGGC
GGCCGGGGAAGTCGGTGCTCTTCCACACGGTGCGCTTTGGCGCGCTCTCT
TGCTGGCTGGGCGAGATGAGCACCGAGCACAGGGGACCCGTGCAGGTGAC
CCAGGTAGAGGAAGATATGGTGATGGTGTTGATGGATGGCTCTGTGAAGC
TGGTGACCCCGGAGGGGTCCCCAGTCCGGGGACTTCGCACACCTGAGATC
CCCATGACCGAAGCGGTGGAGGCCGTGGCTATGGTTGGAGGTCAGCTTCA
GGCCTTCTGGAAGCATGGAGTGCAGGTGTGGGCTCTAGGCTCGGATCAGC
TGCTACAGGAGCTGAGAGACCCTACCCTCACTTTCCGTCTGCTTGGCTCC
CCCAGGCCTGTAGTGGTGGAGACACGCCCAGTGGATGATCCTACTGCTCC
CAGCAACCTCTACATCCAGGAATGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPGIPGSTRAAAMDVVDPDI
FNRDPRDHYDLLQRLGGGTYGEVFKARDKVSGDLVALKMVKMEPDDDVST
LQKEILILKTCRHANIVAYHGSYLWLQKLWICMEFCGAGSLQDIYQVTGS
LSELQISYVCREVLQGLAYLHSQKKIHRDIKGANILINDAGEVRLADFGI
SAQIGATLARRLSFIGTPYWMAPEVAAVALKGGYNELCDIWSLGITAIEL
AELQPPLFDVHPLRVLFLMTKSGYQPPRLKEKGKWSAAFHNFIKVTLTKS
PKKRPSATKMLSHQLVSQPGLNRGLILDLLDKLKNPGKGPSIGDIEDEEP
ELPPAIPRRIRSTHRSSSLGIPDADCCRRHMEFRKLRGMETRPPANTARL
QPPRDLRSSSPRKQLSESSDDDYDDVDIPTPAEDTPPPLPPKPKFRSPSD
EGPGSMGDDGQLSPGVLVRCASGPPPNSPRPGPPPSTSSPHLTAHSEPSL
WNPPSRELDKPPLLPPKKEKMKRKGCALLVKLFNGCPLRIHSTAAWTHPS
TKDQHLLLGAEEGIFILNRNDQEATLEMLFPSRTTWVYSINNVLMSLSGK
TPHLYSHSILGLLERKETRAGNPIAHISPHRLLARKNMVSTKIQDTKGCR
ACCVAEGASSGGPFLCGALETSVVLLQWYQPMNKFLLVRQVLFPLPTPLS
VFALLTGPGSELPAVCIGVSPGRPGKSVLFHTVRFGALSCWLGEMSTEHR
GPVQVTQVEEDMVMVLMDGSVKLVTPEGSPVRGLRTPEIPMTEAVEAVAM
VGGQLQAFWKHGVQVWALGSDQLLQELRDPTLTFRLLGSPRPVVVETRPV
DDPTAPSNLYIQE*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU32901
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for bacterial expression; ; Estimated MW of expressed protein : 118225 Daltons