cDNA Clone SGK2

• Plasmid: pCMV-HA-2 SGK2 alpha
• Parent Plasmid: pCMV5 HA2
• Expressed: HA SGK2 alpha
• Genbank: NM_170693
• Tag: HA
• Species: Human

Sequence of Insert

GGATCCAGCTGCCTGATCATTGCTACAGAATGAACTCTAGCCCAGCTGGG
ACCCCAAGTCCACAGCCCTCCAGGGCCAATGGGAACATCAACCTGGGGCC
TTCAGCCAACCCAAATGCCCAGCCCACGGACTTCGACTTCCTCAAAGTCA
TCGGCAAAGGGAACTACGGGAAGGTCCTACTGGCCAAGCGCAAGTCTGAT
GGGGCGTTCTATGCAGTGAAGGTACTACAGAAAAAGTCCATCTTAAAGAA
GAAAGAGCAGAGCCACATCATGGCAGAGCGCAGTGTGCTTCTGAAGAACG
TGCGGCACCCCTTCCTCGTGGGCCTGCGCTACTCCTTCCAGACACCTGAG
AAGCTCTACTTCGTGCTCGACTATGTCAACGGGGGAGAGCTCTTCTTCCA
CCTGCAGCGGGAGCGCCGGTTCCTGGAGCCCCGGGCCAGGTTCTACGCTG
CTGAGGTGGCCAGCGCCATTGGCTACCTGCACTCCCTCAACATCATTTAC
AGGGATCTGAAACCAGAGAACATTCTCTTGGACTGCCAGGGACACGTGGT
GCTGACGGATTTTGGCCTCTGCAAGGAAGGTGTAGAGCCTGAAGACACCA
CATCCACATTCTGTGGTACCCCTGAGTACTTGGCACCTGAAGTGCTTCGG
AAAGAGCCTTATGATCGAGCAGTGGACTGGTGGTGCTTGGGGGCAGTCCT
CTACGAGATGCTCCATGGCCTGCCGCCCTTCTACAGCCAAGATGTATCCC
AGATGTATGAGAACATTCTGCACCAGCCGCTACAGATCCCCGGAGGCCGG
ACAGTGGCCGCCTGTGACCTCCTGCAAAGCCTTCTCCACAAGGACCAGAG
GCAGCGGCTGGGCTCCAAAGCAGACTTTCTTGAGATTAAGAACCATGTAT
TCTTCAGCCCCATAAACTGGGATGACCTGTACCACAAGAGGCTAACTCCA
CCCTTCAACCCAAATGTGACAGGACCTGCTGACTTGAAGCATTTTGACCC
AGAGTTCACCCAGGAAGCTGTGTCCAAGTCCATTGGCTGTACCCCTGACA
CTGTGGCCAGCAGCTCTGGGGCCTCAAGTGCATTCCTGGGATTTTCTTAT
GCGCCAGAGGATGATGACATCTTGGATTGCTAGGCGGCCGC

Amino Acid Sequence

MYPYDVPDYAGIQLPDHCYRMNSSPAGTPSPQPSRANGNINLGPSANPNA
QPTDFDFLKVIGKGNYGKVLLAKRKSDGAFYAVKVLQKKSILKKKEQSHI
MAERSVLLKNVRHPFLVGLRYSFQTPEKLYFVLDYVNGGELFFHLQRERR
FLEPRARFYAAEVASAIGYLHSLNIIYRDLKPENILLDCQGHVVLTDFGL
CKEGVEPEDTTSTFCGTPEYLAPEVLRKEPYDRAVDWWCLGAVLYEMLHG
LPPFYSQDVSQMYENILHQPLQIPGGRTVAACDLLQSLLHKDQRQRLGSK
ADFLEIKNHVFFSPINWDDLYHKRLTPPFNPNVTGPADLKHFDPEFTQEA
VSKSIGCTPDTVASSSGASSAFLGFSYAPEDDDILDC*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU32892
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST for mammalian expression