cDNA Clone UBE1

• Synonyms: Ubiquitin-Activating Enzyme E1
• Plasmid: pFastBac HTb Ube1
• Parent Plasmid: pFastBac HTb
• Expressed: 6His TEV UBE1
• Genbank: AAA61246.1
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCTCCAGCTCGCCGCTGTCCAAGAAACGTCGCGTGTCCGGGCCTGA
TCCAAAGCCGGGTTCTAACTGCTCCCCTGCCCAGTCCGTGTTGTCCGAAG
TGCCCTCGGTGCCAACCAACGGAATGGCCAAGAACGGCAGTGAAGCAGAC
ATAGACGAGGGCCTTTACTCCCGGCAGCTGTATGTGTTGGGCCATGAGGC
AATGAAGCGGCTCCAGACATCCAGTGTCCTGGTATCAGGCCTGCGGGGCC
TGGGCGTGGAGATCGCTAAGAACATCATCCTTGGTGGGGTCAAGGCTGTT
ACCCTACATGACCAGGGCACTGCCCAGTGGGCTGATCTTTCCTCCCAGTT
CTACCTGCGGGAGGAGGACATCGGTAAAAACCGGGCCGAGGTATCACAGC
CCCGCCTCGCTGAGCTCAACAGCTATGTGCCTGTCACTGCCTACACTGGA
CCCCTCGTTGAGGACTTCCTTAGTGGTTTCCAGGTGGTGGTGCTCACCAA
CACCCCCCTGGAGGACCAGCTGCGAGTGGGTGAGTTCTGTCACAACCGTG
GCATCAAGCTGGTGGTGGCAGACACGCGGGGCCTGTTTGGGCAGCTCTTC
TGTGACTTTGGAGAGGAAATGATCCTCACAGATTCCAATGGGGAGCAGCC
ACTCAGTGCTATGGTTTCTATGGTTACCAAGGACAACCCCGGTGTGGTTA
CCTGCCTGGATGAGGCCCGACACGGGTTTGAGAGCGGGGACTTTGTCTCC
TTTTCAGAAGTACAGGGCATGGTTGAACTCAACGGAAATCAGCCCATGGA
GATCAAAGTCCTGGGTCCTTATACCTTTAGCATCTGTGACACCTCCAACT
TCTCCGACTACATCCGTGGAGGCATCGTCAGTCAGGTCAAAGTACCTAAG
AAGATTAGCTTTAAATCCTTGGTGGCCTCACTGGCAGAACCTGACTTTGT
GGTGACGGACTTCGCCAAGTTTTCTCGCCCTGCCCAGCTGCACATTGGCT
TCCAGGCCCTGCACCAGTTCTGTGCTCAGCATGGCCGGCCACCTCGGCCC
CGCAATGAGGAGGATGCAGCAGAACTGGTAGCCTTAGCACAGGCTGTGAA
TGCTCGAGCCCTGCCAGCAGTGCAGCAAAATAACCTGGACGAGGACCTCA
TCCGGAAGCTGGCATATGTGGCTGCTGGGGATCTGGCACCCATAAACGCC
TTCATTGGGGGCCTGGCTGCCCAGGAAGTCATGAAGGCCTGCTCCGGGAA
GTTCATGCCCATCATGCAGTGGCTATACTTTGATGCCCTTGAGTGTCTCC
CTGAGGACAAAGAGGTCCTCACAGAGGACAAGTGCCTCCAGCGCCAGAAC
CGTTATGACGGGCAAGTGGCTGTGTTTGGCTCAGACCTGCAAGAGAAGCT
GGGCAAGCAGAAGTATTTCCTGGTGGGTGCGGGGGCCATTGGCTGTGAGC
TGCTCAAGAACTTTGCCATGATTGGGCTGGGCTGCGGGGAGGGTGGAGAA
ATCATCGTTACAGACATGGACACCATTGAGAAGTCAAATCTGAATCGACA
GTTTCTTTTCCGGCCCTGGGATGTCACGAAGTTAAAGTCTGACACGGCTG
CTGCAGCTGTGCGCCAAATGAATCCACATATCCGGGTGACAAGCCACCAG
AACCGTGTGGGTCCTGACACGGAGCGCATCTATGATGACGATTTTTTCCA
AAACCTAGATGGCGTGGCCAATGCCCTGGACAACGTGGATGCCCGCATGT
ACATGGACCGCCGCTGTGTCTACTACCGGAAGCCACTGCTGGAGTCAGGC
ACACTGGGCACCAAGGGCAATGTGCAGGTGGTGATCCCCTTCCTGACAGA
GTCGTACAGTTCCAGCCAGGACCCACCTGAGAAGTCCATCCCCATCTGTA
CCCTGAAGAACTTCCCTAATGCCATCGAGCACACCCTGCAGTGGGCTCGG
GATGAGTTTGAAGGCCTCTTCAAGCAGCCAGCAGAAAATGTCAACCAGTA
CCTCACAGACCCCAAGTTTGTGGAGCGAACACTGCGGCTGGCAGGCACTC
AGCCCTTGGAGGTGCTGGAGGCTGTGCAGCGCAGCCTGGTGCTGCAGCGA
CCACAGACCTGGGCTGACTGCGTGACCTGGGCCTGCCACCACTGGCACAC
CCAGTACTCGAACAACATCCGGCAGCTGCTGCACAACTTCCCTCCTGACC
AGCTCACAAGCTCAGGAGCGCCGTTCTGGTCTGGGCCCAAACGCTGTCCA
CACCCGCTCACCTTTGATGTCAACAATCCCCTGCATCTGGACTATGTGAT
GGCTGCTGCCAACCTGTTTGCCCAGACCTACGGGCTGACAGGCTCTCAGG
ACCGAGCTGCTGTGGCCACATTCCTGCAGTCTGTGCAGGTCCCCGAATTC
ACCCCCAAGTCTGGCGTCAAGATCCATGTTTCTGACCAGGAGCTGCAGAG
CGCCAATGCCTCTGTTGATGACAGTCGTCTAGAGGAGCTCAAAGCCACTC
TGCCCAGCCCAGACAAGCTCCCTGGATTCAAGATGTACCCCATTGACTTT
GAGAAGGATGATGACAGCAACTTTCATATGGATTTCATCGTGGCTGCATC
CAACCTCCGGGCAGAAAACTATGACATTCCTTCTGCAGACCGGCACAAGA
GCAAGCTGATTGCAGGGAAGATCATCCCAGCCATTGCCACGACCACAGCA
GCCGTGGTTGGCCTTGTGTGTCTGGAGCTGTACAAGGTTGTGCAGGGGCA
CCGACAGCTTGACTCCTACAAGAATGGTTTCCTCAACTTGGCCCTGCCTT
TCTTTGGTTTCTCTGAACCCCTTGCCGCACCACGTCACCAGTACTATAAC
CAAGAGTGGACATTGTGGGATCGCTTTGAGGTACAAGGGCTGCAGCCTAA
TGGTGAGGAGATGACCCTCAAACAGTTCCTCGACTATTTTAAGACAGAGC
ACAAATTAGAGATCACCATGCTGTCCCAGGGCGTGTCCATGCTCTATTCC
TTCTTCATGCCAGCTGCCAAGCTCAAGGAACGGTTGGATCAGCCGATGAC
AGAGATTGTGAGCCGTGTGTCGAAGCGAAAGCTGGGCCGCCACGTGCGGG
CGCTGGTGCTTGAGCTGTGCTGTAACGACGAGAGCGGCGAGGATGTCGAG
GTTCCCTATGTCCGATACACCATCCGCTGAGGATCC

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGSSSSPLSKKRRVSGPDPKPGSNC
SPAQSVLSEVPSVPTNGMAKNGSEADIDEGLYSRQLYVLGHEAMKRLQTS
SVLVSGLRGLGVEIAKNIILGGVKAVTLHDQGTAQWADLSSQFYLREEDI
GKNRAEVSQPRLAELNSYVPVTAYTGPLVEDFLSGFQVVVLTNTPLEDQL
RVGEFCHNRGIKLVVADTRGLFGQLFCDFGEEMILTDSNGEQPLSAMVSM
VTKDNPGVVTCLDEARHGFESGDFVSFSEVQGMVELNGNQPMEIKVLGPY
TFSICDTSNFSDYIRGGIVSQVKVPKKISFKSLVASLAEPDFVVTDFAKF
SRPAQLHIGFQALHQFCAQHGRPPRPRNEEDAAELVALAQAVNARALPAV
QQNNLDEDLIRKLAYVAAGDLAPINAFIGGLAAQEVMKACSGKFMPIMQW
LYFDALECLPEDKEVLTEDKCLQRQNRYDGQVAVFGSDLQEKLGKQKYFL
VGAGAIGCELLKNFAMIGLGCGEGGEIIVTDMDTIEKSNLNRQFLFRPWD
VTKLKSDTAAAAVRQMNPHIRVTSHQNRVGPDTERIYDDDFFQNLDGVAN
ALDNVDARMYMDRRCVYYRKPLLESGTLGTKGNVQVVIPFLTESYSSSQD
PPEKSIPICTLKNFPNAIEHTLQWARDEFEGLFKQPAENVNQYLTDPKFV
ERTLRLAGTQPLEVLEAVQRSLVLQRPQTWADCVTWACHHWHTQYSNNIR
QLLHNFPPDQLTSSGAPFWSGPKRCPHPLTFDVNNPLHLDYVMAAANLFA
QTYGLTGSQDRAAVATFLQSVQVPEFTPKSGVKIHVSDQELQSANASVDD
SRLEELKATLPSPDKLPGFKMYPIDFEKDDDSNFHMDFIVAASNLRAENY
DIPSADRHKSKLIAGKIIPAIATTTAAVVGLVCLELYKVVQGHRQLDSYK
NGFLNLALPFFGFSEPLAAPRHQYYNQEWTLWDRFEVQGLQPNGEEMTLK
QFLDYFKTEHKLEITMLSQGVSMLYSFFMPAAKLKERLDQPMTEIVSRVS
KRKLGRHVRALVLELCCNDESGEDVEVPYVRYTIR*

• Vector Type: Baculovirus
• Antibiotic: Amp
• Full Snapgene Map: pFastBac HTb Ube1127.48 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU32888
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Nter 6HIS for baculovirus expression
• Low Temp: No