cDNA Clone JAK3

• Plasmid: pFBDual 6P-1 JAK3
• Parent Plasmid: pFastbacDual 6P1
• Expressed: GST JAK3
• Genbank: NP_000206.2
• Tag: GST
• Species: Human

Sequence of Insert

GAATTCATGGCACCTCCAAGTGAAGAGACGCCCCTGATCCCTCAGCGTTC
ATGCAGCCTCTTGTCCACGGAGGCTGGTGCCCTGCATGTGCTGCTGCCCG
CTCGGGGCCCCGGGCCCCCCCAGCGCCTATCTTTCTCCTTTGGGGACCAC
TTGGCTGAGGACCTGTGCGTGCAGGCTGCCAAGGCCAGCGGCATCCTGCC
TGTGTACCACTCCCTCTTTGCTCTGGCCACGGAGGACCTGTCCTGCTGGT
TCCCCCCGAGCCACATCTTCTCCGTGGAGGATGCCAGCACCCAAGTCCTG
CTGTACAGGATTCGCTTTTACTTCCCCAATTGGTTTGGGCTGGAGAAGTG
CCACCGCTTCGGGCTACGCAAGGATTTGGCCAGTGCTATCCTTGACCTGC
CAGTCCTGGAGCACCTCTTTGCCCAGCACCGCAGTGACCTGGTGAGTGGG
CGCCTCCCCGTGGGCCTCAGTCTCAAGGAGCAGGGTGAGTGTCTCAGCCT
GGCCGTGTTGGACCTGGCCCGGATGGCGCGAGAGCAGGCCCAGCGGCCGG
GAGAGCTGCTGAAGACTGTCAGCTACAAGGCCTGCCTACCCCCAAGCCTG
CGCGACCTGATCCAGGGCCTGAGCTTCGTGACGCGGAGGCGTATTCGGAG
GACGGTGCGCAGAGCCCTGCGCCGCGTGGCCGCCTGCCAGGCAGACCGGC
ACTCGCTCATGGCCAAGTACATCATGGACCTGGAGCGGCTGGATCCAGCC
GGGGCCGCCGAGACCTTCCACGTGGGCCTCCCTGGGGCCCTTGGTGGCCA
CGACGGGCTGGGGCTGCTCCGCGTGGCTGGTGACGGCGGCATCGCCTGGA
CCCAGGGAGAACAGGAGGTCCTCCAGCCCTTCTGCGACTTTCCAGAAATC
GTAGACATTAGCATCAAGCAGGCCCCACGCGTTGGCCCGGCCGGAGAGCA
CCGCCTGGTCACTGTTACCAGGACAGACAACCAGATTTTAGAGGCCGAGT
TCCCAGGGCTGCCCGAGGCTCTGTCGTTCGTGGCGCTCGTGGACGGCTAC
TTCCGGCTGACCACGGACTCCCAGCACTTCTTCTGCAAGGAGGTGGCACC
GCCGAGGCTGCTGGAGGAAGTGGCCGAGCAGTGCCACGGCCCCATCACTC
TGGACTTTGCCATCAACAAGCTCAAGACTGGGGGCTCACGTCCTGGCTCC
TATGTTCTCCGCCGCAGCCCCCAGGACTTTGACAGCTTCCTCCTCACTGT
CTGTGTCCAGAACCCCCTTGGTCCTGATTATAAGGGCTGCCTCATCCGGC
GCAGCCCCACAGGAACCTTCCTTCTGGTTGGCCTCAGCCGACCCCACAGC
AGTCTTCGAGAGCTCCTGGCAACCTGCTGGGATGGGGGGCTGCACGTAGA
TGGGGTGGCAGTGACCCTCACTTCCTGCTGTATCCCCAGACCCAAAGAAA
AGTCCAACCTGATCGTGGTCCAGAGAGGTCACAGCCCACCCACATCATCC
TTGGTTCAGCCCCAATCCCAATACCAGCTGAGTCAGATGACATTTCACAA
GATCCCTGCTGACAGCCTGGAGTGGCATGAGAACCTGGGCCATGGGTCCT
TCACCAAGATTTACCGGGGCTGTCGCCATGAGGTGGTCGACGGGGAGGCC
CGAAAGACAGAGGTGCTGCTGAAGGTCATGGATGCCAAGCACAAGAACTG
CATGGAGTCATTCCTGGAAGCAGCGAGCTTGATGAGCCAAGTGTCGTACC
GGCATCTCGTGCTGCTCCACGGCGTGTGCATGGCTGGAGACAGCACCATG
GTGCAGGAATTTGTACACCTGGGGGCCATAGACATGTATCTGCGAAAACG
TGGCCACCTGGTGCCAGCCAGCTGGAAGCTGCAGGTGGTCAAACAGCTGG
CCTACGCCCTCAACTATCTGGAGGACAAAGGCCTGCCCCATGGCAATGTC
TCTGCCCGGAAGGTGCTCCTGGCTCGGGAGGGGGCTGATGGGAGCCCGCC
CTTCATCAAGCTGAGTGACCCTGGGGTCAGCCCCGCTGTGTTAAGCCTGG
AGATGCTCACCGACAGGATCCCCTGGGTGGCCCCCGAGTGTCTCCGGGAG
GCGCAGACACTTAGCTTGGAAGCTGACAAGTGGGGCTTCGGCGCCACGGT
CTGGGAAGTGTTTAGTGGCGTCACCATGCCCATCAGTGCCCTGGATCCTG
CTAAGAAACTCCAATTTTATGAGGACCGGCAGCAGCTGCCGGCCCCCAAG
TGGACAGAGCTGGCCCTGCTGATTCAACAGTGCATGGCCTATGAGCCGGT
CCAGAGGCCCTCCTTCCGAGCCGTCATTCGTGACCTCAATAGCCTCATCT
CTTCAGACTATGAGCTCCTCTCAGACCCCACACCTGGTGCCCTGGCACCT
CGTGATGGGCTGTGGAATGGTGCCCAGCTCTATGCCTGCCAAGACCCCAC
GATCTTCGAGGAGAGACACCTCAAGTACATCTCACAGCTGGGCAAGGGCA
ACTTTGGCAGCGTGGAGCTGTGCCGCTATGACCCGCTAGGCGACAATACA
GGTGCCCTGGTGGCCGTGAAACAGCTGCAGCACAGCGGGCCAGACCAGCA
GAGGGACTTTCAGCGGGAGATTCAGATCCTCAAAGCACTGCACAGTGATT
TCATTGTCAAGTATCGTGGTGTCAGCTATGGCCCGGGCCGCCAGAGCCTG
CGGCTGGTCATGGAGTACCTGCCCAGCGGCTGCTTGCGCGACTTCCTGCA
GCGGCACCGCGCGCGCCTCGATGCCAGCCGCCTCCTTCTCTATTCCTCGC
AGATCTGCAAGGGCATGGAGTACCTGGGCTCCCGCCGCTGCGTGCACCGC
GACCTGGCCGCCCGAAACATCCTCGTGGAGAGCGAGGCACACGTCAAGAT
CGCTGACTTCGGCCTAGCTAAGCTGCTGCCGCTTGACAAAGACTACTACG
TGGTCCGCGAGCCAGGCCAGAGCCCCATTTTCTGGTATGCCCCCGAATCC
CTCTCGGACAACATCTTCTCTCGCCAGTCAGACGTCTGGAGCTTCGGGGT
CGTCCTGTACGAGCTCTTCACCTACTGCGACAAAAGCTGCAGCCCCTCGG
CCGAGTTCCTGCGGATGATGGGATGTGAGCGGGATGTCCCCGCCCTCTGC
CGCCTCTTGGAACTGCTGGAGGAGGGCCAGAGGCTGCCGGCGCCTCCTGC
CTGCCCTGCTGAGGTTCACGAGCTCATGAAGCTGTGCTGGGCCCCTAGCC
CACAGGACCGGCCATCATTCAGCGCCCTGGGCCCCCAGCTGGACATGCTG
TGGAGCGGAAGCCGGGGGTGTGAGACTCATGCCTTCACTGCTCACCCAGA
GGGCAAACACCACTCCCTGTCCTTTTCATAGGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPEFMAPPSEETPLIPQRSC
SLLSTEAGALHVLLPARGPGPPQRLSFSFGDHLAEDLCVQAAKASGILPV
YHSLFALATEDLSCWFPPSHIFSVEDASTQVLLYRIRFYFPNWFGLEKCH
RFGLRKDLASAILDLPVLEHLFAQHRSDLVSGRLPVGLSLKEQGECLSLA
VLDLARMAREQAQRPGELLKTVSYKACLPPSLRDLIQGLSFVTRRRIRRT
VRRALRRVAACQADRHSLMAKYIMDLERLDPAGAAETFHVGLPGALGGHD
GLGLLRVAGDGGIAWTQGEQEVLQPFCDFPEIVDISIKQAPRVGPAGEHR
LVTVTRTDNQILEAEFPGLPEALSFVALVDGYFRLTTDSQHFFCKEVAPP
RLLEEVAEQCHGPITLDFAINKLKTGGSRPGSYVLRRSPQDFDSFLLTVC
VQNPLGPDYKGCLIRRSPTGTFLLVGLSRPHSSLRELLATCWDGGLHVDG
VAVTLTSCCIPRPKEKSNLIVVQRGHSPPTSSLVQPQSQYQLSQMTFHKI
PADSLEWHENLGHGSFTKIYRGCRHEVVDGEARKTEVLLKVMDAKHKNCM
ESFLEAASLMSQVSYRHLVLLHGVCMAGDSTMVQEFVHLGAIDMYLRKRG
HLVPASWKLQVVKQLAYALNYLEDKGLPHGNVSARKVLLAREGADGSPPF
IKLSDPGVSPAVLSLEMLTDRIPWVAPECLREAQTLSLEADKWGFGATVW
EVFSGVTMPISALDPAKKLQFYEDRQQLPAPKWTELALLIQQCMAYEPVQ
RPSFRAVIRDLNSLISSDYELLSDPTPGALAPRDGLWNGAQLYACQDPTI
FEERHLKYISQLGKGNFGSVELCRYDPLGDNTGALVAVKQLQHSGPDQQR
DFQREIQILKALHSDFIVKYRGVSYGPGRQSLRLVMEYLPSGCLRDFLQR
HRARLDASRLLLYSSQICKGMEYLGSRRCVHRDLAARNILVESEAHVKIA
DFGLAKLLPLDKDYYVVREPGQSPIFWYAPESLSDNIFSRQSDVWSFGVV
LYELFTYCDKSCSPSAEFLRMMGCERDVPALCRLLELLEEGQRLPAPPAC
PAEVHELMKLCWAPSPQDRPSFSALGPQLDMLWSGSRGCETHAFTAHPEG
KHHSLSFS*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU32832
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for baculovirus expression; ; Estimated MW of expressed protein : 152291 Daltons