cDNA Clone IPAH9.8

• Plasmid: pEBG6P-IPAH9.8
• Parent Plasmid: pEBG6P
• Expressed: GST IPAH9.8
• Genbank: AY206445.1
• Tag: GST
• Species: Shigella flexneri

Sequence of Insert

GGATCCATGTTACCGATAAATAATAACTTTTCATTGCCCCAAAATTCTTT
TTATAACACTATTTCCGGTACATATGCTGATTACTTTTCAGCATGGGATA
AATGGGAAAAACAAGCGCTCCCCGGTGAAGAGCGTGATGAGGCTGTCTCC
CGACTTAAAGAATGTCTTATCAATAATTCCGATGAACTTCGACTGGACCG
TTTAAATCTGTCCTCGCTACCTGACAACTTACCAGCTCAGATAACGCTGC
TCAATGTATCATATAATCAATTAACTAACCTACCTGAACTGCCTGTTACG
CTAAAAAAATTATATTCCGCCAGCAATAAATTATCAGAATTGCCCGTGCT
ACCTCCTGCGCTGGAGTCACTTCAGGTACAACACAATGAGCTGGAAAACC
TGCCAGCTTTACCCGATTCGTTATTGACTATGAATATCAGCTATAACGAA
ATAGTCTCCTTACCATCGCTCCCACAGGCTCTTAAAAATCTCAGAGCGAC
CCGTAATTTCCTCACTGAGCTACCAGCATTTTCTGAGGGAAATAATCCCG
TTGTCAGAGAGTATTTTTTTGATAGAAATCAGATAAGTCATATCCCGGAA
AGCATTCTTAATCTGAGGAATGAATGTTCAATACATATTAGTGATAACCC
ATTATCATCCCATGCTCTGCAAGCCCTGCAAAGATTAACCTCTTCGCCGG
ACTACCACGGCCCACGGATTTACTTCTCCATGAGTGACGGACAACAGAAT
ACACTCCATCGCCCCCTGGCTGATGCCGTGACAGCATGGTTCCCGGAAAA
CAAACAATCTGATGTATCACAGATATGGCATGCTTTTGAACATGAAGAGC
ATGCCAACACCTTTTCCGCGTTCCTTGACCGCCTTTCCGATACCGTCTCT
GCACGCAATACCTCCGGATTCCGTGAACAGGTCGCTGCATGGCTGGAAAA
ACTCAGTGCCTCTGCGGAGCTTCGACAGCAGTCTTTCGCTGTTGCTGCTG
ATGCCACTGAGAGCTGTGAGGACCGTGTCGCGCTCACATGGAACAATCTC
CGGAAAACCCTCCTGGTCCATCAGGCATCAGAAGGCCTTTTCGATAATGA
TACCGGCGCTCTGCTCTCCCTGGGCAGGGAAATGTTCCGCCTCGAAATTC
TGGAGGACATTGCCCGGGATAAAGTCAGAACTCTCCATTTTGTGGATGAG
ATAGAAGTCTACCTGGCCTTCCAGACCATGCTCGCAGAGAAACTTCAGCT
CTCCACTGCCGTGAAGGAAATGCGTTTCTATGGCGTGTCGGGAGTGACAG
CAAATGACCTCCGCACTGCCGAAGCCATGGTCAGAAGCCGTGAAGAGAAT
GAATTTACGGACTGGTTCTCCCTCTGGGGACCATGGCATGCTGTACTGAA
GCGTACGGAAGCTGACCGCTGGGCGCTGGCAGAAGAGCAGAAATATGAGA
TGCTGGAGAATGAGTACCCTCAGAGGGTGGCTGACCGGCTGAAAGCATCA
GGTCTGAGCGGTGATGCGGATGCGGAGAGGGAAGCCGGTGCACAGGTGAT
GCGTGAGACTGAACAGCAGATTTACCGTCAGCTGACTGACGAGGTACTGG
CCCTGCGATTGCCTGAAAACGGCTCACAACTGCACCATTCATAAGCGGCC
GC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMLPINNNFSLPQN
SFYNTISGTYADYFSAWDKWEKQALPGEERDEAVSRLKECLINNSDELRL
DRLNLSSLPDNLPAQITLLNVSYNQLTNLPELPVTLKKLYSASNKLSELP
VLPPALESLQVQHNELENLPALPDSLLTMNISYNEIVSLPSLPQALKNLR
ATRNFLTELPAFSEGNNPVVREYFFDRNQISHIPESILNLRNECSIHISD
NPLSSHALQALQRLTSSPDYHGPRIYFSMSDGQQNTLHRPLADAVTAWFP
ENKQSDVSQIWHAFEHEEHANTFSAFLDRLSDTVSARNTSGFREQVAAWL
EKLSASAELRQQSFAVAADATESCEDRVALTWNNLRKTLLVHQASEGLFD
NDTGALLSLGREMFRLEILEDIARDKVRTLHFVDEIEVYLAFQTMLAEKL
QLSTAVKEMRFYGVSGVTANDLRTAEAMVRSREENEFTDWFSLWGPWHAV
LKRTEADRWALAEEQKYEMLENEYPQRVADRLKASGLSGDADAEREAGAQ
VMRETEQQIYRQLTDEVLALRLPENGSQLHHS*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU32827
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for mammalian expression; ; Estimated MW of expressed protein : 89409 Daltons