cDNA Clone IPAH9.8

• Plasmid: pGEX6P-1-IPAH9.8
• Parent Plasmid: pGEX6P1
• Expressed: GST IPAH9.8
• Genbank: AY206445.1
• Tag: GST
• Species: Shigella flexneri

Sequence of Insert

GGATCCATGTTACCGATAAATAATAACTTTTCATTGCCCCAAAATTCTTT
TTATAACACTATTTCCGGTACATATGCTGATTACTTTTCAGCATGGGATA
AATGGGAAAAACAAGCGCTCCCCGGTGAAGAGCGTGATGAGGCTGTCTCC
CGACTTAAAGAATGTCTTATCAATAATTCCGATGAACTTCGACTGGACCG
TTTAAATCTGTCCTCGCTACCTGACAACTTACCAGCTCAGATAACGCTGC
TCAATGTATCATATAATCAATTAACTAACCTACCTGAACTGCCTGTTACG
CTAAAAAAATTATATTCCGCCAGCAATAAATTATCAGAATTGCCCGTGCT
ACCTCCTGCGCTGGAGTCACTTCAGGTACAACACAATGAGCTGGAAAACC
TGCCAGCTTTACCCGATTCGTTATTGACTATGAATATCAGCTATAACGAA
ATAGTCTCCTTACCATCGCTCCCACAGGCTCTTAAAAATCTCAGAGCGAC
CCGTAATTTCCTCACTGAGCTACCAGCATTTTCTGAGGGAAATAATCCCG
TTGTCAGAGAGTATTTTTTTGATAGAAATCAGATAAGTCATATCCCGGAA
AGCATTCTTAATCTGAGGAATGAATGTTCAATACATATTAGTGATAACCC
ATTATCATCCCATGCTCTGCAAGCCCTGCAAAGATTAACCTCTTCGCCGG
ACTACCACGGCCCACGGATTTACTTCTCCATGAGTGACGGACAACAGAAT
ACACTCCATCGCCCCCTGGCTGATGCCGTGACAGCATGGTTCCCGGAAAA
CAAACAATCTGATGTATCACAGATATGGCATGCTTTTGAACATGAAGAGC
ATGCCAACACCTTTTCCGCGTTCCTTGACCGCCTTTCCGATACCGTCTCT
GCACGCAATACCTCCGGATTCCGTGAACAGGTCGCTGCATGGCTGGAAAA
ACTCAGTGCCTCTGCGGAGCTTCGACAGCAGTCTTTCGCTGTTGCTGCTG
ATGCCACTGAGAGCTGTGAGGACCGTGTCGCGCTCACATGGAACAATCTC
CGGAAAACCCTCCTGGTCCATCAGGCATCAGAAGGCCTTTTCGATAATGA
TACCGGCGCTCTGCTCTCCCTGGGCAGGGAAATGTTCCGCCTCGAAATTC
TGGAGGACATTGCCCGGGATAAAGTCAGAACTCTCCATTTTGTGGATGAG
ATAGAAGTCTACCTGGCCTTCCAGACCATGCTCGCAGAGAAACTTCAGCT
CTCCACTGCCGTGAAGGAAATGCGTTTCTATGGCGTGTCGGGAGTGACAG
CAAATGACCTCCGCACTGCCGAAGCCATGGTCAGAAGCCGTGAAGAGAAT
GAATTTACGGACTGGTTCTCCCTCTGGGGACCATGGCATGCTGTACTGAA
GCGTACGGAAGCTGACCGCTGGGCGCTGGCAGAAGAGCAGAAATATGAGA
TGCTGGAGAATGAGTACCCTCAGAGGGTGGCTGACCGGCTGAAAGCATCA
GGTCTGAGCGGTGATGCGGATGCGGAGAGGGAAGCCGGTGCACAGGTGAT
GCGTGAGACTGAACAGCAGATTTACCGTCAGCTGACTGACGAGGTACTGG
CCCTGCGATTGCCTGAAAACGGCTCACAACTGCACCATTCATAAGCGGCC
GC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMLPINNNFSLPQNSFYNTI
SGTYADYFSAWDKWEKQALPGEERDEAVSRLKECLINNSDELRLDRLNLS
SLPDNLPAQITLLNVSYNQLTNLPELPVTLKKLYSASNKLSELPVLPPAL
ESLQVQHNELENLPALPDSLLTMNISYNEIVSLPSLPQALKNLRATRNFL
TELPAFSEGNNPVVREYFFDRNQISHIPESILNLRNECSIHISDNPLSSH
ALQALQRLTSSPDYHGPRIYFSMSDGQQNTLHRPLADAVTAWFPENKQSD
VSQIWHAFEHEEHANTFSAFLDRLSDTVSARNTSGFREQVAAWLEKLSAS
AELRQQSFAVAADATESCEDRVALTWNNLRKTLLVHQASEGLFDNDTGAL
LSLGREMFRLEILEDIARDKVRTLHFVDEIEVYLAFQTMLAEKLQLSTAV
KEMRFYGVSGVTANDLRTAEAMVRSREENEFTDWFSLWGPWHAVLKRTEA
DRWALAEEQKYEMLENEYPQRVADRLKASGLSGDADAEREAGAQVMRETE
QQIYRQLTDEVLALRLPENGSQLHHS*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU32826
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for bacterial expression; ; Estimated MW of expressed protein : 88816 Daltons