cDNA Clone LEF1

• Plasmid: pGEX6P-1-LEF1
• Parent Plasmid: pGEX6P1
• Expressed: GST LEF1
• Genbank: NP_057353.1
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGCCCCAACTCTCCGGAGGAGGTGGCGGCGGCGGGGGGGACCC
GGAACTCTGCGCCACGGACGAGATGATCCCCTTCAAGGACGAGGGCGATC
CTCAGAAGGAAAAGATCTTCGCCGAGATCAGTCATCCCGAAGAGGAAGGC
GATTTAGCTGACATCAAGTCTTCCTTGGTGAACGAGTCTGAAATCATCCC
GGCCAGCAACGGACACGAGGTGGCCAGACAAGCACAAACCTCTCAGGAGC
CCTACCACGACAAGGCCAGAGAACACCCCGATGACGGAAAGCATCCAGAT
GGAGGCCTCTACAACAAGGGACCCTCCTACTCGAGTTATTCCGGGTACAT
AATGATGCCAAATATGAATAACGACCCATACATGTCAAATGGATCTCTTT
CTCCACCCATCCCGAGAACATCAAATAAAGTGCCCGTGGTGCAGCCATCC
CATGCGGTCCATCCTCTCACCCCCCTCATCACTTACAGTGACGAGCACTT
TTCTCCAGGATCACACCCGTCACACATCCCATCAGATGTCAACTCCAAAC
AAGGCATGTCCAGACATCCTCCAGCTCCTGATATCCCTACTTTTTATCCC
TTGTCTCCGGGTGGTGTTGGACAGATCACCCCACCTCTTGGCTGGCAAGG
TCAGCCTGTATATCCCATCACGGGTGGATTCAGGCAACCCTACCCATCCT
CACTGTCAGTCGACACTTCCATGTCCAGGTTTTCCCATCATATGATTCCC
GGTCCTCCTGGTCCCCACACAACTGGCATCCCTCATCCAGCTATTGTAAC
ACCTCAGGTCAAACAGGAACATCCCCACACTGACAGTGACCTAATGCACG
TGAAGCCTCAGCATGAACAGAGAAAGGAGCAGGAGCCAAAAAGACCTCAC
ATTAAGAAGCCTCTGAATGCTTTTATGTTATACATGAAAGAAATGAGAGC
GAATGTCGTTGCTGAGTGTACTCTAAAAGAAAGTGCAGCTATCAACCAGA
TTCTTGGCAGAAGGTGGCATGCCCTCTCCCGTGAAGAGCAGGCTAAATAT
TATGAATTAGCACGGAAAGAAAGACAGCTACATATGCAGCTTTATCCAGG
CTGGTCTGCAAGAGACAATTATGGTAAGAAAAAGAAGAGGAAGAGAGAGA
AACTACAGGAATCTGCATCAGGTACAGGTCCAAGAATGACAGCTGCCTAC
ATCTGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMPQLSGGGGGGGGDPELCA
TDEMIPFKDEGDPQKEKIFAEISHPEEEGDLADIKSSLVNESEIIPASNG
HEVARQAQTSQEPYHDKAREHPDDGKHPDGGLYNKGPSYSSYSGYIMMPN
MNNDPYMSNGSLSPPIPRTSNKVPVVQPSHAVHPLTPLITYSDEHFSPGS
HPSHIPSDVNSKQGMSRHPPAPDIPTFYPLSPGGVGQITPPLGWQGQPVY
PITGGFRQPYPSSLSVDTSMSRFSHHMIPGPPGPHTTGIPHPAIVTPQVK
QEHPHTDSDLMHVKPQHEQRKEQEPKRPHIKKPLNAFMLYMKEMRANVVA
ECTLKESAAINQILGRRWHALSREEQAKYYELARKERQLHMQLYPGWSAR
DNYGKKKKRKREKLQESASGTGPRMTAAYI*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU32823
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for bacterial expression; ; Estimated MW of expressed protein : 71023 Daltons