cDNA Clone SENP1

• Plasmid: pFBDual6P-1 SENP1
• Parent Plasmid: pFastbacDual 6P1
• Expressed: GST SENP1
• Genbank: AK292060.1
• Tag: GST
• Species: Human

Sequence of Insert

GAATTCATGGATGATATTGCTGATAGGATGAGGATGGATGCTGGAGAAGT
GACTTTAGTGAACCACAACTCCGTATTCAAAACCCACCTCCTGCCACAAA
CAGGTTTTCCAGAGGACCAGCTTTCGCTTTCTGACCAGCAGATTTTATCT
TCCAGGCAAGGACATTTGGACCGATCTTTTACATGTTCCACAAGAAGTGC
AGCTTATAATCCAAGCTATTACTCAGATAATCCTTCCTCAGACAGTTTTC
TTGGCTCAGGCGATTTAAGAACCTTTGGCCAGAGTGCAAATGGCCAATGG
AGAAATTCTACCCCATCGTCAAGCTCATCTTTACAAAAATCAAGAAACAG
CCGAAGTCTTTACCTCGAAACCCGAAAGACCTCAAGTGGATTATCAAACA
GTTTTGCGGGAAAGTCAAACCATCACTGCCATGTATCTGCATATGAAAAA
TCTTTTCCTATTAAACCTGTTCCAAGTCCATCTTGGAGTGGTTCATGTCG
TCGAAGTCTCTTGAGCCCCAAGAAAACTCAGAGGCGACATGTTAGTACAG
CAGAAGAGACAGTTCAAGAAGAAGAAAGAGAGATTTACAGACAGCTGCTA
CAGATGGTCACAGGGAAACAGTTTACTATAGCCAAACCCACCACACATTT
TCCTTTACACCTGTCTCGATGTCTTAGTTCCAGTAAAAATACTTTGAAAG
ACTCACTGTTTAAAAATGGAAACTCTTGTGCATCTCAGATCATTGGCTCT
GATACTTCATCATCTGGATCTGCCAGCATTTTAACTAACCAGGAACAGCT
GTCCCACAGTGTATATTCCCTATCTTCTTATACCCCAGATGTTGCATTTG
GATCCAAAGATTCTGGTACTCTTCATCATCCCCATCATCACCACTCTGTT
CCACATCAGCCAGATAACTTAGCAGCTTCAAATACACAATCTGAAGGATC
AGACTCTGTGATTTTACTGAAAGTGAAAGATTCCCAGACTCCAACTCCCA
GTTCTACTTTCTTCCAGGCAGAGCTGTGGATCAAAGAATTAACTAGTGTT
TATGATTCTCGAGCACGAGAAAGATTGCGCCAGATTGACGAACAGAAGGC
ATTGGCCTTACAGCTTCAAAACCAGAGATTGCAGGAGCGGGAACATTCAG
TACATGATTCAGTAGAACTACATCTTCGTGTACCTCTTGAAAAGGAGATT
CCTGTTACTGTTGTCCAAGAAACACAAAAAAAAGGTCATAAATTAACTGA
TAGTGAAGATGAATTTCCTGAAATTACAGAGGAAATGGAGAAAGAAATAA
AGAATGTATTTCGTAATGGGAATCAGGATGAAGTTCTCAGTGAAGCATTT
CGCCTGACCATTACACGCAAAGATATTCAAACTCTAAACCATCTGAATTG
GCTCAATGATGAGATCATCAATTTCTACATGAATATGCTGATGGAGCGAA
GTAAAGAGAAGGGCTTGCCAAGTGTGCATGCATTTAATACCTTTTTCTTC
ACTAAATTAAAAACGGCTGGTTATCAGGCAGTGAAACGTTGGACAAAGAA
AGTAGATGTATTTTCTGTTGACATTCTTTTGGTGCCCATTCACCTGGGAG
TACACTGGTGTCTAGCTGTTGTGGACTTTAGAAAGAAGAATATTACCTAT
TACGACTCCATGGGTGGGATAAACAATGAAGCCTGCAGAATACTCTTGCA
ATACCTAAAGCAAGAAAGCATTGACAAGAAAAGGAAAGAGTTTGACACCA
ATGGCTGGCAGCTTTTCAGCAAGAAAAGCCAGGAGATTCCTCAGCAGATG
AATGGAAGTGACTGTGGGATGTTTGCCTGCAAATATGCTGACTGTATTAC
CAAAGACAGACCAATCAACTTCACACAGCAACACATGCCATACTTCCGGA
AGCGGATGGTCTGGGAGATCCTCCACCGAAAACTCTTGTGAGTCGAC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPEFMDDIADRMRMDAGEVT
LVNHNSVFKTHLLPQTGFPEDQLSLSDQQILSSRQGHLDRSFTCSTRSAA
YNPSYYSDNPSSDSFLGSGDLRTFGQSANGQWRNSTPSSSSSLQKSRNSR
SLYLETRKTSSGLSNSFAGKSNHHCHVSAYEKSFPIKPVPSPSWSGSCRR
SLLSPKKTQRRHVSTAEETVQEEEREIYRQLLQMVTGKQFTIAKPTTHFP
LHLSRCLSSSKNTLKDSLFKNGNSCASQIIGSDTSSSGSASILTNQEQLS
HSVYSLSSYTPDVAFGSKDSGTLHHPHHHHSVPHQPDNLAASNTQSEGSD
SVILLKVKDSQTPTPSSTFFQAELWIKELTSVYDSRARERLRQIDEQKAL
ALQLQNQRLQEREHSVHDSVELHLRVPLEKEIPVTVVQETQKKGHKLTDS
EDEFPEITEEMEKEIKNVFRNGNQDEVLSEAFRLTITRKDIQTLNHLNWL
NDEIINFYMNMLMERSKEKGLPSVHAFNTFFFTKLKTAGYQAVKRWTKKV
DVFSVDILLVPIHLGVHWCLAVVDFRKKNITYYDSMGGINNEACRILLQY
LKQESIDKKRKEFDTNGWQLFSKKSQEIPQQMNGSDCGMFACKYADCITK
DRPINFTQQHMPYFRKRMVWEILHRKLL*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU32820
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for insect expression. Sequence agrees with genbank except for E361D change which occurs in the original Hay construct.; ; Estimated MW of expressed protein : 100662 Daltons