cDNA Clone SENP2

• Plasmid: pFBHTa SENP2
• Parent Plasmid: pSC-A
• Expressed: 6His SENP2
• Genbank: AK304402.1
• Tag: 6His
• Species: Human

Sequence of Insert

GAATTCATGTACAGATGGCTGGTTAGGATTCTCGGCACCATTTTCCGTTT
CTGCGACCGGTCGGTGCCCCCTGCCCGGGCCCTCCTGAAGAGGCGGCGCT
CAGACAGCACTCTGTTTTCTACAGTGGACACTGATGAAATACCAGCCAAA
AGACCAAGATTAGATTGCTTTATTCACCAAGTGAAAAACAGTCTCTACAA
TGCTGCCAGCTTATTTGGATTCCCATTCCAGCTGACCACAAAGCCCATGG
TAACTTCTGCTTGTAATGGAACACGGAATGTGGCCCCTTCAGGAGAGGTA
TTTTCGAACTCTTCATCTTGTGAACTGACAGGTTCTGGATCCTGGAACAA
CATGCTGAAACTGGGTAATAAATCTCCTAATGGAATAAGTGACTATCCAA
AGATCAGAGTGACAGTTACCCGAGATCAGCCACGCAGAGTCCTGCCTTCC
TTTGGTTTTACTTTGAACTCAGAAGGCTGTAATAGAAGACCAGGTGGCCG
TCGCCATAGCAAAGGTAATCCAGAGAGTTCTTTAATGTGGAAACCTCGGG
AACAGGCTGTAACAGAGATGATTTCTGAAGAGAGTGGCAAGGGTCTGAGG
CGTCCCCATTGTACTGTGGAGGAGGGTGTTCAAAAAGAGGAAAGAGAGAA
GTACCGAAAGTTATTGGAACGACTTAAAGAAAGTGGTCATGGAAACTCTG
TCTGTCCTGTAACTTCAAATTATCACAGTTCTCAAAGAAGTCAGATGGAC
ACATTAAAGACCAAAGGCTGGGGGGAAGAGCAAAATCACGGAGTCAAAAC
AACTCAGTTTGTTCCAAAACAATATAGACTTGTTGAAACAAGGGGACCTC
TATGTTCATTGAGAAGTGAAAAGAGGTGTTCAAAGGGGAAAATTACTGAT
ACAGAGACGATGGTCGGAATCAGATTTGAAAATGAAAGTAGGAGGGGATA
CCAACTGGAGCCTGACCTATCAGAAGAAGTGTCGGCCCGACTCCGCCTGG
GCAGTGGAAGCAATGGCTTACTCAGGAGGAAAGTGTCAATAATTGAGACA
AAGGAAAAGAATTGCTCAGGCAAAGAGAGGGACAGAAGAACGGACGATCT
CCTTGAACTTACAGAGGACATGGAAAAGGAAATCAGTAATGCCCTAGGCC
ATGGCCCACAGGATGAAATCCTAAGTAGTGCTTTCAAATTGCGAATTACT
CGAGGAGATATTCAGACATTAAAGAACTATCACTGGCTCAATGATGAAGT
CATTAATTTTTACATGAATCTTCTGGTGGAAAGAAATAAAAAGCAAGGCT
ATCCAGCACTTCATGTATTCAGTACTTTCTTCTATCCTAAATTAAAGTCT
AGGGGTTACCAAGCAGTGAAACGATGGACCAAAGGGGTAAATCTCTTTGA
ACAAGAAATTATTCTGGTGCCTATTCATCGGAAGGTACATTGGAGCCTGG
TGGTGATTGACCTAAGAAAAAAGTGTCTTAAATATCTGGATTCTATGGGA
CAAAAGGGCCACAGGATCTGTGAGATTCTCCTTCAGTATTTACAGGATGA
AAGTAAGACCAAAAGAAATAGTGATCTGAATCTTTTAGAGTGGACCCATC
ACAGCATGAAACCACACGAGATTCCTCAACAGCTGAATGGGAGTGATTGT
GGAATGTTTACTTGTAAATATGCAGATTATATTTCTAGGGACAAACCTAT
CACATTTACTCAGCACCAGATGCCTCTCTTCCGGAAGAAGATGGTGTGGG
AAATCCTTCATCAGCAGTTGCTGTGAGTCGAC

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMDPEFMYRWLVRILGTIFRFCDRSV
PPARALLKRRRSDSTLFSTVDTDEIPAKRPRLDCFIHQVKNSLYNAASLF
GFPFQLTTKPMVTSACNGTRNVAPSGEVFSNSSSCELTGSGSWNNMLKLG
NKSPNGISDYPKIRVTVTRDQPRRVLPSFGFTLNSEGCNRRPGGRRHSKG
NPESSLMWKPREQAVTEMISEESGKGLRRPHCTVEEGVQKEEREKYRKLL
ERLKESGHGNSVCPVTSNYHSSQRSQMDTLKTKGWGEEQNHGVKTTQFVP
KQYRLVETRGPLCSLRSEKRCSKGKITDTETMVGIRFENESRRGYQLEPD
LSEEVSARLRLGSGSNGLLRRKVSIIETKEKNCSGKERDRRTDDLLELTE
DMEKEISNALGHGPQDEILSSAFKLRITRGDIQTLKNYHWLNDEVINFYM
NLLVERNKKQGYPALHVFSTFFYPKLKSRGYQAVKRWTKGVNLFEQEIIL
VPIHRKVHWSLVVIDLRKKCLKYLDSMGQKGHRICEILLQYLQDESKTKR
NSDLNLLEWTHHSMKPHEIPQQLNGSDCGMFTCKYADYISRDKPITFTQH
QMPLFRKKMVWEILHQQLL*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU32814
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter 6HIS for baculovirus expression. Sequence agrees with genbank except for G449R change which occurs in the original Hay construct.; ; Estimated MW of expressed protein : 71697 Daltons