cDNA Clone SIK2

• Synonyms: SIK2
• Plasmid: pcDNA5-FRT/TO-FLAG-2 QIK S358A
• Parent Plasmid: pcDNA5 FRT/TO FLAG2
• Expressed: FLAG QIK S358A
• Genbank: NP_056006.1
• Tag: FLAG
• Species: Human

Sequence of Insert

GCGGCCGCGATGGTCATGGCGGATGGCCCGAGGCACTTGCAGCGCGGGCC
GGTCCGGGTGGGGTTCTACGACATCGAGGGCACGCTGGGCAAGGGCAACT
TCGCTGTGGTGAAGCTGGGGCGGCACCGGATCACCAAGACGGAGGTGGCA
ATAAAAATAATCGATAAGTCTCAGCTGGATGCAGTGAACCTTGAGAAAAT
CTACCGAGAAGTACAAATAATGAAAATGTTAGACCACCCTCACATAATCA
AACTTTATCAGGTAATGGAGACCAAAAGTATGTTGTACCTTGTGACAGAA
TATGCCAAAAATGGAGAAATTTTTGACTATCTTGCTAATCATGGCCGGTT
AAATGAGTCTGAAGCCAGGCGAAAATTCTGGCAAATCCTGTCTGCTGTTG
ATTATTGTCATGGTCGGAAGATTGTGCACCGTGACCTCAAAGCTGAAAAT
CTCCTGCTGGATAACAACATGAATATCAAAATAGCAGATTTCGGTTTTGG
AAATTTCTTTAAAAGTGGTGAACTGCTGGCAACATGGTGTGGCAGCCCCC
CTTATGCAGCCCCAGAAGTCTTTGAAGGGCAGCAGTATGAAGGACCACAG
CTGGACATCTGGAGTATGGGAGTTGTTCTTTATGTCCTTGTCTGTGGAGC
TCTGCCCTTTGATGGACCGACTCTTCCAATTTTGAGGCAGAGGGTTCTGG
AAGGAAGATTCCGGATTCCGTATTTCATGTCAGAAGATTGCGAGCACCTT
ATCCGAAGGATGTTGGTCCTAGACCCATCCAAACGGCTAACCATAGCCCA
AATCAAGGAGCATAAATGGATGCTCATAGAAGTTCCTGTCCAGAGACCTG
TTCTCTATCCACAAGAGCAAGAAAATGAGCCATCCATCGGGGAGTTTAAT
GAGCAGGTTCTGCGACTGATGCACAGCCTTGGAATAGATCAGCAGAAAAC
CATTGAGTCTTTGCAGAACAAGAGCTATAACCACTTTGCTGCCATTTATT
TCTTGTTGGTGGAGCGCCTGAAATCACATCGGAGCAGTTTCCCAGTGGAG
CAGAGACTTGATGGCCGCCAGCGTCGGCCTGCCACCATTGCTGAGCAAAC
AGTTGCCAAGGCACAGACTGTGGGGCTCCCAGTGACCATGCATTCACCGA
ACATGAGGCTGCTGCGATCTGCCCTCCTCCCCCAGGCATCCAACGTGGAG
GCCTTTTCATTTCCAGCATCTGGCTGTCAGGCGGAAGCTGCATTCATGGA
AGAAGAGTGTGTGGACACTCCAAAGGTCAATGGCTGTCTGCTTGACCCTG
TGCCTCCTGTCCTGGTGCGGAAGGGATGCCAGTCACTGCCCAGCAACATG
ATGGAGACCTCCATTGACGAAGGGCTGGAGACAGAAGGAGAGGCCGAGGA
AGACCCCGCTCATGCCTTTGAGGCATTTCAGTCCACACGCAGCGGGCAGA
GACGGCACACTCTGTCAGAAGTGACCAATCAACTGGTCGTGATGCCTGGG
GCAGGGAAAATTTTCTCCATGAATGACAGCCCCTCCCTTGACAGTGTGGA
CTCTGAGTATGATATGGGGTCTGTTCAGAGGGACCTGAACTTTCTGGAAG
ACAACCCTTCCCTTAAGGACATCATGTTAGCCAATCAGCCTTCACCCCGC
ATGACATCTCCCTTCATAAGCCTGAGACCTACCAACCCAGCCATGCAGGC
TCTGAGCTCCCAGAAACGAGAGGTCCACAACAGGTCTCCAGTGAGCTTCA
GAGAGGGCCGCAGAGCATCAGATACCTCCCTCACCCAGGGAATTGTAGCA
TTTAGACAACATCTTCAGAATCTGGCTAGAACCAAAGGAATTCTAGAGTT
GAACAAAGTGCAGTTGTTGTATGAACAAATAGGACCGGAGGCAGACCCTA
ACCTGGCGCCGGCGGCTCCTCAGCTCCAGGACCTTGCTAGCAGCTGCCCT
CAGGAAGAAGTTTCTCAGCAGCAGGAAAGCGTCTCCACTCTCCCTGCCAG
CGTGCATCCCCAGCTGTCCCCACGGCAGAGCCTGGAGACCCAGTACCTGC
AGCACAGACTCCAGAAGCCCAGCCTTCTGTCAAAGGCCCAGAACACCTGT
CAGCTTTATTGCAAAGAACCACCGCGGAGCCTTGAGCAGCAGCTGCAGGA
ACATAGGCTCCAGCAGAAGCGACTCTTTCTTCAGAAGCAGTCTCAACTGC
AGGCCTATTTTAATCAGATGCAGATAGCAGAGAGCTCCTACCCACAGCCA
AGTCAGCAGCTGCCCCTTCCCCGCCAGGAGACTCCACCGCCTTCTCAGCA
GGCCCCACCGTTCAGCCTGACCCAGCCCCTGAGCCCCGTCCTGGAGCCTT
CCTCCGAGCAGATGCAATACAGCCCTTTCCTCAGCCAGTACCAAGAGATG
CAGCTTCAGCCCCTGCCCTCCACTTCCGGTCCCCGGGCTGCTCCTCCTCT
GCCCACGCAGCTACAGCAGCAGCAGCCGCCACCGCCACCACCCCCTCCAC
CACCACGACAGCCAGGAGCTGCCCCAGCCCCCTTACAGTTCTCCTATCAG
ACTTGTGAGCTGCCAAGCGCTGCTTCCCCTGCGCCAGACTATCCCACTCC
CTGTCAGTATCCTGTGGATGGAGCCCAGCAGAGCGACCTAACGGGGCCAG
ACTGTCCCAGAAGCCCAGGACTGCAAGAGGCCCCCTCCAGCTACGACCCA
CTAGCCCTCTCTGAGCTACCTGGACTCTTTGATTGTGAAATGCTAGACGC
TGTGGATCCACAACACAACGGGTATGTCCTGGTGAATTAGGCGGCCGC

Amino Acid Sequence

MDYKDDDDKGIPGIPGSTRAAAMVMADGPRHLQRGPVRVGFYDIEGTLGK
GNFAVVKLGRHRITKTEVAIKIIDKSQLDAVNLEKIYREVQIMKMLDHPH
IIKLYQVMETKSMLYLVTEYAKNGEIFDYLANHGRLNESEARRKFWQILS
AVDYCHGRKIVHRDLKAENLLLDNNMNIKIADFGFGNFFKSGELLATWCG
SPPYAAPEVFEGQQYEGPQLDIWSMGVVLYVLVCGALPFDGPTLPILRQR
VLEGRFRIPYFMSEDCEHLIRRMLVLDPSKRLTIAQIKEHKWMLIEVPVQ
RPVLYPQEQENEPSIGEFNEQVLRLMHSLGIDQQKTIESLQNKSYNHFAA
IYFLLVERLKSHRSSFPVEQRLDGRQRRPATIAEQTVAKAQTVGLPVTMH
SPNMRLLRSALLPQASNVEAFSFPASGCQAEAAFMEEECVDTPKVNGCLL
DPVPPVLVRKGCQSLPSNMMETSIDEGLETEGEAEEDPAHAFEAFQSTRS
GQRRHTLSEVTNQLVVMPGAGKIFSMNDSPSLDSVDSEYDMGSVQRDLNF
LEDNPSLKDIMLANQPSPRMTSPFISLRPTNPAMQALSSQKREVHNRSPV
SFREGRRASDTSLTQGIVAFRQHLQNLARTKGILELNKVQLLYEQIGPEA
DPNLAPAAPQLQDLASSCPQEEVSQQQESVSTLPASVHPQLSPRQSLETQ
YLQHRLQKPSLLSKAQNTCQLYCKEPPRSLEQQLQEHRLQQKRLFLQKQS
QLQAYFNQMQIAESSYPQPSQQLPLPRQETPPPSQQAPPFSLTQPLSPVL
EPSSEQMQYSPFLSQYQEMQLQPLPSTSGPRAAPPLPTQLQQQQPPPPPP
PPPPRQPGAAPAPLQFSYQTCELPSAASPAPDYPTPCQYPVDGAQQSDLT
GPDCPRSPGLQEAPSSYDPLALSELPGLFDCEMLDAVDPQHNGYVLVN*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU32756
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N TER FLAG FOR MAMMALIAN EXPRESSION ; ; Estimated MW of expressed protein : 106171 Daltons