cDNA Clone PHLPP1

• Plasmid: pCMV-HA-2 PHLPP1 1-600
• Parent Plasmid: pCMV5 HA2
• Expressed: HA PHLPP1 1-600
• Genbank: NP_919431
• Tag: HA
• Species: Human

Sequence of Insert

GCGGCCGCGATGGACTCGGAGATTGGCTGCCTCATCCGCTTCTATGCAGG
AAAACCTCACAGCACGGGTAGCTCTGAACGGATTCAGCTCTCAGGAATGT
ATAATGTCCGTAAAGGCAAGATGCAGTTGCCAGTGAACCGATGGACAAGA
CGCCAAGTCATCCTATGTGGGACCTGCCTGATAGTATCATCTGTGAAAGA
CAGCTTGACCGGAAAGATGCATGTTCTGCCACTAATTGGTGGAAAAGTAG
AAGAAGTGAAAAAGCACCAACACTGTTTAGCATTTAGCTCCTCTGGACCC
CAAAGCCAGACTTACTACATTTGCTTTGATACTTTCACAGAATACTTAAG
GTGGCTGCGACAAGTCTCCAAGGTTGCATCCCAGCGCATTAGCTCAGTGG
ACCTCTCGTGTTGTAGCCTGGAACATCTGCCTGCCAACCTCTTCTACAGC
CAAGACCTCACTCATCTCAATTTAAAACAAAACTTCCTAAGGCAGAACCC
TAGCCTTCCAGCTGCCAGGGGGCTTAATGAACTGCAAAGGTTCACCAAGT
TGAAGAGTCTTAACCTTTCCAATAATCATTTAGGGGACTTCCCACTGGCA
GTCTGCAGTATTCCAACCCTGGCAGAGCTGAACGTGTCCTGCAATGCCCT
GCGATCAGTCCCGGCAGCCGTTGGAGTGATGCACAACTTACAGACATTTT
TGTTGGATGGAAACTTTCTCCAATCCCTTCCTGCTGAGTTGGAGAACATG
AAGCAGCTTAGTTATCTGGGTCTTTCTTTCAATGAATTTACTGACATTCC
CGAAGTATTGGAGAAATTGACTGCTGTGGATAAACTTTGTATGTCTGGAA
ACTGTGTGGAGACCCTTAGGCTACAGGCTTTAAGAAAAATGCCTCACATT
AAACATGTGGATCTAAGGTTGAACGTAATTAGGAAGCTGATAGCAGATGA
AGTGGACTTTCTACAGCATGTTACTCAGCTTGACCTACGAGACAATAAGC
TTGGTGATCTAGATGCTATGATTTTCAACAACATTGAAGTTTTACACTGT
GAAAGGAATCAACTGGTCACATTAGACATCTGTGGCTATTTCCTAAAAGC
GCTCTATGCCTCTTCTAATGAACTTGTTCAACTTGATGTTTACCCAGTTC
CAAATTATCTGTCCTACATGGATGTTTCAAGGAACCGCTTAGAAAATGTG
CCTGAGTGGGTATGTGAAAGCCGAAAGCTAGAAGTTTTGGATATTGGCCA
TAATCAAATATGTGAACTTCCTGCCCGCTTATTTTGTAATAGCAGTCTCC
GGAAACTACTGGCAGGACACAACCAGTTGGCAAGGCTGCCTGAAAGGCTA
GAAAGAACCTCGGTGGAGGTCTTGGATGTGCAACACAACCAGCTCCTTGA
GCTCCCACCTAACCTTCTGATGAAGGCTGACAGCCTGAGATTCCTGAACG
CCTCTGCGAACAAACTGGAAAGCCTTCCTCCAGCCACGCTTTCCGAAGAG
ACAAACAGTATCTTACAAGAGTTGTATTTGACAAATAACAGCCTCACAGA
CAAATGTGTGCCCTTGTTAACGGGACACCCCCATTTGAAGATCCTTCACA
TGGCCTATAACCGACTTCAGAGTTTTCCAGCAAGTAAAATGGCGAAACTG
GAGGAACTTGAAGAAATTGATCTCAGTGGGAATAAGCTGAAAGCCATCCC
AACAACGATCATGAATTGCAGGCGCATGCACACCGTGATTGCTCACTCCA
ACTGCATCGAGGTCTTTCCCGAAGTTATGCAGCTCCCAGAGATCAAGTGT
GTGGACCTGTAAGCGGCCGC

Amino Acid Sequence

MYPYDVPDYAGIPGIPGSTRAAAMDSEIGCLIRFYAGKPHSTGSSERIQL
SGMYNVRKGKMQLPVNRWTRRQVILCGTCLIVSSVKDSLTGKMHVLPLIG
GKVEEVKKHQHCLAFSSSGPQSQTYYICFDTFTEYLRWLRQVSKVASQRI
SSVDLSCCSLEHLPANLFYSQDLTHLNLKQNFLRQNPSLPAARGLNELQR
FTKLKSLNLSNNHLGDFPLAVCSIPTLAELNVSCNALRSVPAAVGVMHNL
QTFLLDGNFLQSLPAELENMKQLSYLGLSFNEFTDIPEVLEKLTAVDKLC
MSGNCVETLRLQALRKMPHIKHVDLRLNVIRKLIADEVDFLQHVTQLDLR
DNKLGDLDAMIFNNIEVLHCERNQLVTLDICGYFLKALYASSNELVQLDV
YPVPNYLSYMDVSRNRLENVPEWVCESRKLEVLDIGHNQICELPARLFCN
SSLRKLLAGHNQLARLPERLERTSVEVLDVQHNQLLELPPNLLMKADSLR
FLNASANKLESLPPATLSEETNSILQELYLTNNSLTDKCVPLLTGHPHLK
ILHMAYNRLQSFPASKMAKLEELEEIDLSGNKLKAIPTTIMNCRRMHTVI
AHSNCIEVFPEVMQLPEIKCVDL*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU32728
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter HA for mammalian expression; ; Estimated MW of expressed protein : 70271 Daltons