cDNA Clone PHLPP1

• Plasmid: pCMV-HA-2 PHLPP1 150-600
• Parent Plasmid: pCMV5 HA2
• Expressed: HA PHLPP1 150-600
• Genbank: NP_919431
• Tag: HA
• Species: Human

Sequence of Insert

GCGGCCGCGCTCACTCATCTCAATTTAAAACAAAACTTCCTAAGGCAGAA
CCCTAGCCTTCCAGCTGCCAGGGGGCTTAATGAACTGCAAAGGTTCACCA
AGTTGAAGAGTCTTAACCTTTCCAATAATCATTTAGGGGACTTCCCACTG
GCAGTCTGCAGTATTCCAACCCTGGCAGAGCTGAACGTGTCCTGCAATGC
CCTGCGATCAGTCCCGGCAGCCGTTGGAGTGATGCACAACTTACAGACAT
TTTTGTTGGATGGAAACTTTCTCCAATCCCTTCCTGCTGAGTTGGAGAAC
ATGAAGCAGCTTAGTTATCTGGGTCTTTCTTTCAATGAATTTACTGACAT
TCCCGAAGTATTGGAGAAATTGACTGCTGTGGATAAACTTTGTATGTCTG
GAAACTGTGTGGAGACCCTTAGGCTACAGGCTTTAAGAAAAATGCCTCAC
ATTAAACATGTGGATCTAAGGTTGAACGTAATTAGGAAGCTGATAGCAGA
TGAAGTGGACTTTCTACAGCATGTTACTCAGCTTGACCTACGAGACAATA
AGCTTGGTGATCTAGATGCTATGATTTTCAACAACATTGAAGTTTTACAC
TGTGAAAGGAATCAACTGGTCACATTAGACATCTGTGGCTATTTCCTAAA
AGCGCTCTATGCCTCTTCTAATGAACTTGTTCAACTTGATGTTTACCCAG
TTCCAAATTATCTGTCCTACATGGATGTTTCAAGGAACCGCTTAGAAAAT
GTGCCTGAGTGGGTATGTGAAAGCCGAAAGCTAGAAGTTTTGGATATTGG
CCATAATCAAATATGTGAACTTCCTGCCCGCTTATTTTGTAATAGCAGTC
TCCGGAAACTACTGGCAGGACACAACCAGTTGGCAAGGCTGCCTGAAAGG
CTAGAAAGAACCTCGGTGGAGGTCTTGGATGTGCAACACAACCAGCTCCT
TGAGCTCCCACCTAACCTTCTGATGAAGGCTGACAGCCTGAGATTCCTGA
ACGCCTCTGCGAACAAACTGGAAAGCCTTCCTCCAGCCACGCTTTCCGAA
GAGACAAACAGTATCTTACAAGAGTTGTATTTGACAAATAACAGCCTCAC
AGACAAATGTGTGCCCTTGTTAACGGGACACCCCCATTTGAAGATCCTTC
ACATGGCCTATAACCGACTTCAGAGTTTTCCAGCAAGTAAAATGGCGAAA
CTGGAGGAACTTGAAGAAATTGATCTCAGTGGGAATAAGCTGAAAGCCAT
CCCAACAACGATCATGAATTGCAGGCGCATGCACACCGTGATTGCTCACT
CCAACTGCATCGAGGTCTTTCCCGAAGTTATGCAGCTCCCAGAGATCAAG
TGTGTGGACCTGTAAGCGGCCGC

Amino Acid Sequence

MYPYDVPDYAGIPGIPGSTRAAALTHLNLKQNFLRQNPSLPAARGLNELQ
RFTKLKSLNLSNNHLGDFPLAVCSIPTLAELNVSCNALRSVPAAVGVMHN
LQTFLLDGNFLQSLPAELENMKQLSYLGLSFNEFTDIPEVLEKLTAVDKL
CMSGNCVETLRLQALRKMPHIKHVDLRLNVIRKLIADEVDFLQHVTQLDL
RDNKLGDLDAMIFNNIEVLHCERNQLVTLDICGYFLKALYASSNELVQLD
VYPVPNYLSYMDVSRNRLENVPEWVCESRKLEVLDIGHNQICELPARLFC
NSSLRKLLAGHNQLARLPERLERTSVEVLDVQHNQLLELPPNLLMKADSL
RFLNASANKLESLPPATLSEETNSILQELYLTNNSLTDKCVPLLTGHPHL
KILHMAYNRLQSFPASKMAKLEELEEIDLSGNKLKAIPTTIMNCRRMHTV
IAHSNCIEVFPEVMQLPEIKCVDL*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU32722
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter HA for bacterial expression; ; Estimated MW of expressed protein : 53418 Daltons