cDNA Clone PHLPP1

• Plasmid: pEBG6P-PHLPP1 1-600
• Parent Plasmid: pEBG6P
• Expressed: GST PHLPP1 1-600
• Genbank: NP_919431
• Tag: GST
• Species: Human

Sequence of Insert

GCGGCCGCGATGGACTCGGAGATTGGCTGCCTCATCCGCTTCTATGCAGG
AAAACCTCACAGCACGGGTAGCTCTGAACGGATTCAGCTCTCAGGAATGT
ATAATGTCCGTAAAGGCAAGATGCAGTTGCCAGTGAACCGATGGACAAGA
CGCCAAGTCATCCTATGTGGGACCTGCCTGATAGTATCATCTGTGAAAGA
CAGCTTGACCGGAAAGATGCATGTTCTGCCACTAATTGGTGGAAAAGTAG
AAGAAGTGAAAAAGCACCAACACTGTTTAGCATTTAGCTCCTCTGGACCC
CAAAGCCAGACTTACTACATTTGCTTTGATACTTTCACAGAATACTTAAG
GTGGCTGCGACAAGTCTCCAAGGTTGCATCCCAGCGCATTAGCTCAGTGG
ACCTCTCGTGTTGTAGCCTGGAACATCTGCCTGCCAACCTCTTCTACAGC
CAAGACCTCACTCATCTCAATTTAAAACAAAACTTCCTAAGGCAGAACCC
TAGCCTTCCAGCTGCCAGGGGGCTTAATGAACTGCAAAGGTTCACCAAGT
TGAAGAGTCTTAACCTTTCCAATAATCATTTAGGGGACTTCCCACTGGCA
GTCTGCAGTATTCCAACCCTGGCAGAGCTGAACGTGTCCTGCAATGCCCT
GCGATCAGTCCCGGCAGCCGTTGGAGTGATGCACAACTTACAGACATTTT
TGTTGGATGGAAACTTTCTCCAATCCCTTCCTGCTGAGTTGGAGAACATG
AAGCAGCTTAGTTATCTGGGTCTTTCTTTCAATGAATTTACTGACATTCC
CGAAGTATTGGAGAAATTGACTGCTGTGGATAAACTTTGTATGTCTGGAA
ACTGTGTGGAGACCCTTAGGCTACAGGCTTTAAGAAAAATGCCTCACATT
AAACATGTGGATCTAAGGTTGAACGTAATTAGGAAGCTGATAGCAGATGA
AGTGGACTTTCTACAGCATGTTACTCAGCTTGACCTACGAGACAATAAGC
TTGGTGATCTAGATGCTATGATTTTCAACAACATTGAAGTTTTACACTGT
GAAAGGAATCAACTGGTCACATTAGACATCTGTGGCTATTTCCTAAAAGC
GCTCTATGCCTCTTCTAATGAACTTGTTCAACTTGATGTTTACCCAGTTC
CAAATTATCTGTCCTACATGGATGTTTCAAGGAACCGCTTAGAAAATGTG
CCTGAGTGGGTATGTGAAAGCCGAAAGCTAGAAGTTTTGGATATTGGCCA
TAATCAAATATGTGAACTTCCTGCCCGCTTATTTTGTAATAGCAGTCTCC
GGAAACTACTGGCAGGACACAACCAGTTGGCAAGGCTGCCTGAAAGGCTA
GAAAGAACCTCGGTGGAGGTCTTGGATGTGCAACACAACCAGCTCCTTGA
GCTCCCACCTAACCTTCTGATGAAGGCTGACAGCCTGAGATTCCTGAACG
CCTCTGCGAACAAACTGGAAAGCCTTCCTCCAGCCACGCTTTCCGAAGAG
ACAAACAGTATCTTACAAGAGTTGTATTTGACAAATAACAGCCTCACAGA
CAAATGTGTGCCCTTGTTAACGGGACACCCCCATTTGAAGATCCTTCACA
TGGCCTATAACCGACTTCAGAGTTTTCCAGCAAGTAAAATGGCGAAACTG
GAGGAACTTGAAGAAATTGATCTCAGTGGGAATAAGCTGAAAGCCATCCC
AACAACGATCATGAATTGCAGGCGCATGCACACCGTGATTGCTCACTCCA
ACTGCATCGAGGTCTTTCCCGAAGTTATGCAGCTCCCAGAGATCAAGTGT
GTGGACCTGTAAGCGGCCGC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSTSGTIDAAAMDSE
IGCLIRFYAGKPHSTGSSERIQLSGMYNVRKGKMQLPVNRWTRRQVILCG
TCLIVSSVKDSLTGKMHVLPLIGGKVEEVKKHQHCLAFSSSGPQSQTYYI
CFDTFTEYLRWLRQVSKVASQRISSVDLSCCSLEHLPANLFYSQDLTHLN
LKQNFLRQNPSLPAARGLNELQRFTKLKSLNLSNNHLGDFPLAVCSIPTL
AELNVSCNALRSVPAAVGVMHNLQTFLLDGNFLQSLPAELENMKQLSYLG
LSFNEFTDIPEVLEKLTAVDKLCMSGNCVETLRLQALRKMPHIKHVDLRL
NVIRKLIADEVDFLQHVTQLDLRDNKLGDLDAMIFNNIEVLHCERNQLVT
LDICGYFLKALYASSNELVQLDVYPVPNYLSYMDVSRNRLENVPEWVCES
RKLEVLDIGHNQICELPARLFCNSSLRKLLAGHNQLARLPERLERTSVEV
LDVQHNQLLELPPNLLMKADSLRFLNASANKLESLPPATLSEETNSILQE
LYLTNNSLTDKCVPLLTGHPHLKILHMAYNRLQSFPASKMAKLEELEEID
LSGNKLKAIPTTIMNCRRMHTVIAHSNCIEVFPEVMQLPEIKCVDL*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU32720
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for mammalian expression; ; Estimated MW of expressed protein : 96112 Daltons