cDNA Clone preScission Protease

• Plasmid: pMalp2X preScission Protease
• Parent Plasmid: pMALP2X
• Expressed: MBP preScission Protease
• Tag: MBP

Sequence of Insert

GGATCCGGTGGAGGACCAAACACAGAATTTGCACTATCCCTGTTAAGGAA
AAACATAATGACTATAACAACCTCAAAGGGAGAGTTCACAGGGTTAGGCA
TACATGATCGTGTCTGTGTGATACCCACACACGCACAGCCTGGTGATGAT
GTACTAGTAAATGGTCAGAAAATTAGAGTTAAGGATAAGTACAAATTAGT
AGATCCAGAGAACATTAATCTAGAGCTTACAGTGTTGACTTTAGATAGAA
ATGAAAAATTCAGAGATATCAGGGGATTTATATCAGAAGATCTAGAAGGT
GTGGATGCCACTTTGGTAGTACATTCAAATAACTTTACCAACACTATCTT
AGAAGTTGGCCCTGTAACAATGGCAGGACTTATTAATTTGAGTAGCACCC
CCACTAACAGAATGATTCGTTATGATTATGCAACAAAAACTGGGCAGTGT
GGAGGTGTGCTGTGTGCTACTGGTAAGATCTTTGGTATTCATGTTGGCGG
TAATGGAAGACAAGGATTTTCAGCTCAACTTAAAAAACAATATTTTGTAG
AGAAACAATGAGTCGAC

Amino Acid Sequence

MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYNGLAEVG
KKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSG
LLAEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPN
PPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENG
KYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGETAM
TINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKE
LAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENA
QKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQTNSSSNNNN
NNNNNNLGIEGRISEFGSGGGPNTEFALSLLRKNIMTITTSKGEFTGLGI
HDRVCVIPTHAQPGDDVLVNGQKIRVKDKYKLVDPENINLELTVLTLDRN
EKFRDIRGFISEDLEGVDATLVVHSNNFTNTILEVGPVTMAGLINLSSTP
TNRMIRYDYATKTGQCGGVLCATGKIFGIHVGGNGRQGFSAQLKKQYFVE
KQ*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU32719
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter MBP for bacterial expression; ; Estimated MW of expressed protein : 65703 Daltons