cDNA Clone B galactosidase

• Synonyms: GLB1
• Plasmid: pGEX6P-1-B galactosidase delta 11-41
• Parent Plasmid: pGEX6P1
• Expressed: GST B galactosidase delta 11-41
• Genbank: BAD83864
• Tag: GST

Sequence of Insert

GAATTCGCCCTTACCATGATTACGGATTCACTGGCGGTCGTTTTACAACG
TCGTGACCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCTTTG
CCTGGTTTCCGGCACCAGAAGCGGTGCCGGAAAGCTGGCTGGAGTGCGAT
CTTCCTGAGGCCGATACTGTCGTCGTCCCCTCAAACTGGCAGATGCACGG
TTACGATGCGCCCATCTACACCAACGTAACCTATCCCATTACGGTCAATC
CGCCGTTTGTTCCCACGGAGAATCCGACGGGTTGTTACTCGCTCACATTT
AATGTTGATGAAAGCTGGCTACAGGAAGGCCAGACGCGAATTATTTTTGA
TGGCGTTAACTCGGCGTTTCATCTGTGGTGCAACGGGCGCTGGGTCGGTT
ACGGCCAGGACAGTCGTTTGCCGTCTGAATTTGACCTGAGCGCATTTTTA
CGCGCCGGAGAAAACCGCCTCGCGGTGATGGTGCTGCGTTGGAGTGACGG
CAGTTATCTGGAAGATCAGGATATGTGGCGGATGAGCGGCATTTTCCGTG
ACGTCTCGTTGCTGCATAAACCGACTACACAAATCAGCGATTTCCATGTT
GCCACTCGCTTTAATGATGATTTCAGCCGCGCTGTACTGGAGGCTGAAGT
TCAGATGTGCGGCGAGTTGCGTGACTACCTACGGGTAACAGTTTCTTTAT
GGCAGGGTGAAACGCAGGTCGCCAGCGGCACCGCGCCTTTCGGCGGTGAA
ATTATCGATGAGCGTGGTGGTTATGCCGATCGCGTCACACTACGTCTGAA
CGTCGAAAACCCGAAACTGTGGAGCGCCGAAATCCCGAATCTCTATCGTG
CGGTGGTTGAACTGCACACCGCCGACGGCACGCTGATTGAAGCAGAAGCC
TGCGATGTCGGTTTCCGCGAGGTGCGGATTGAAAATGGTCTGCTGCTGCT
GAACGGCAAGCCGTTGCTGATTCGAGGCGTTAACCGTCACGAGCATCATC
CTCTGCATGGTCAGGTCATGGATGAGCAGACGATGGTGCAGGATATCCTG
CTGATGAAGCAGAACAACTTTAACGCCGTGCGCTGTTCGCATTATCCGAA
CCATCCGCTGTGGTACACGCTGTGCGACCGCTACGGCCTGTATGTGGTGG
ATGAAGCCAATATTGAAACCCACGGCATGGTGCCAATGAATCGTCTGACC
GATGATCCGCGCTGGCTACCGGCGATGAGCGAACGCGTAACGCGAATGGT
GCAGCGCGATCGTAATCACCCGAGTGTGATCATCTGGTCGCTGGGGAATG
AATCAGGCCACGGCGCTAATCACGACGCGCTGTATCGCTGGATCAAATCT
GTCGATCCTTCCCGCCCGGTGCAGTATGAAGGCGGCGGAGCCGACACCAC
GGCCACCGATATTATTTGCCCGATGTACGCGCGCGTGGATGAAGACCAGC
CCTTCCCGGCTGTGCCGAAATGGTCCATCAAAAAATGGCTTTCGCTACCT
GGAGAGACGCGCCCGCTGATCCTTTGCGAATACGCCCACGCGATGGGTAA
CAGTCTTGGCGGTTTCGCTAAATACTGGCAGGCGTTTCGTCAGTATCCCC
GTTTACAGGGCGGCTTCGTCTGGGACTGGGTGGATCAGTCGCTGATTAAA
TATGATGAAAACGGCAACCCGTGGTCGGCTTACGGCGGTGATTTTGGCGA
TACGCCGAACGATCGCCAGTTCTGTATGAACGGTCTGGTCTTTGCCGACC
GCACGCCGCATCCAGCGCTGACGGAAGCAAAACACCAGCAGCAGTTTTTC
CAGTTCCGTTTATCCGGGCAAACCATCGAAGTGACCAGCGAATACCTGTT
CCGTCATAGCGATAACGAGCTCCTGCACTGGATGGTGGCGCTGGATGGTA
AGCCGCTGGCAAGCGGTGAAGTGCCTCTGGATGTCGCTCCACAAGGTAAA
CAGTTGATTGAACTGCCTGAACTACCGCAGCCGGAGAGCGCCGGGCAACT
CTGGCTCACAGTACGCGTAGTGCAACCGAACGCGACCGCATGGTCAGAAG
CCGGGCACATCAGCGCCTGGCAGCAGTGGCGTCTGGCGGAAAACCTCAGT
GTGACGCTCCCCGCCGCGTCCCACGCCATCCCGCATCTGACCACCAGCGA
AATGGATTTTTGCATCGAGCTGGGTAATAAGCGTTGGCAATTTAACCGCC
AGTCAGGCTTTCTTTCACAGATGTGGATTGGCGATAAAAAACAACTGCTG
ACGCCGCTGCGCGATCAGTTCACCCGTGCACCGCTGGATAACGACATTGG
CGTAAGTGAAGCGACCCGCATTGACCCTAACGCCTGGGTCGAACGCTGGA
AGGCGGCGGGCCATTACCAGGCCGAAGCAGCGTTGTTGCAGTGCACGGCA
GATACACTTGCTGATGCGGTGCTGATTACGACCGCTCACGCGTGGCAGCA
TCAGGGGAAAACCTTATTTATCAGCCGGAAAACCTACCGGATTGATGGTA
GTGGTCAAATGGCGATTACCGTTGATGTTGAAGTGGCGAGCGATACACCG
CATCCGGCGCGGATTGGCCTGAACTGCCAGCTGGCGCAGGTAGCAGAGCG
GGTAAACTGGCTCGGATTAGGGCCGCAAGAAAACTATCCCGACCGCCTTA
CTGCCGCCTGTTTTGACCGCTGGGATCTGCCATTGTCAGACATGTATACC
CCGTACGTCTTCCCGAGCGAAAACGGTCTGCGCTGCGGGACGCGCGAATT
GAATTATGGCCCACACCAGTGGCGCGGCGACTTCCAGTTCAACATCAGCC
GCTACAGTCAACAGCAACTGATGGAAACCAGCCATCGCCATCTGCTGCAC
GCGGAAGAAGGCACATGGCTGAATATCGACGGTTTCCATATGGGGATTGG
TGGCGACGACTCCTGGAGCCCGTCAGTATCGGCGGAGTTCCAGCTGAGCG
CCGGTCGCTACCATTACCAGTTGGTCTGGTGTCAAAAATAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPEFALTMITDSLAVVLQRR
DPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGY
DAPIYTNVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDG
VNSAFHLWCNGRWVGYGQDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGS
YLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATRFNDDFSRAVLEAEVQ
MCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRVTLRLNV
ENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLN
GKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNH
PLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQ
RDRNHPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTA
TDIICPMYARVDEDQPFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGNS
LGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNPWSAYGGDFGDT
PNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYLFR
HSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLW
LTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEM
DFCIELGNKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGV
SEATRIDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQ
GKTLFISRKTYRIDGSGQMAITVDVEVASDTPHPARIGLNCQLAQVAERV
NWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLRCGTRELN
YGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGG
DDSWSPSVSAEFQLSAGRYHYQLVWCQK*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU32705
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for bacterial expression; ; Estimated MW of expressed protein : 140055 Daltons