cDNA Clone USP7

• Plasmid: pEBG6P-USP7 50-550
• Parent Plasmid: pEBG6P
• Expressed: GST USP7 50-550
• Genbank: NP_003461.2
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCGATGGACACAACACCGCGGAGGAGGACATGGAGGATGACACCAG
TTGGCGCTCCGAGGCAACCTTTCAGTTCACTGTGGAGCGCTTCAGCAGAC
TGAGTGAGTCGGTCCTTAGCCCTCCGTGTTTTGTGCGAAATCTGCCATGG
AAGATTATGGTGATGCCACGCTTTTATCCAGACAGACCACACCAAAAAAG
CGTAGGATTCTTTCTCCAGTGCAATGCTGAATCTGATTCCACGTCATGGT
CTTGCCATGCACAAGCAGTGCTGAAGATAATAAATTACAGAGATGATGAA
AAGTCGTTCAGTCGTCGTATTAGTCATTTGTTCTTCCATAAAGAAAATGA
TTGGGGATTTTCCAATTTTATGGCCTGGAGTGAAGTGACCGATCCTGAGA
AAGGATTTATAGATGATGACAAAGTTACCTTTGAAGTCTTTGTACAGGCG
GATGCTCCCCATGGAGTTGCGTGGGATTCAAAGAAGCACACAGGCTACGT
CGGCTTAAAGAATCAGGGAGCGACTTGTTACATGAACAGCCTGCTACAGA
CGTTATTTTTCACGAATCAGCTACGAAAGGCTGTGTACATGATGCCAACC
GAGGGGGATGATTCGTCTAAAAGCGTCCCTTTAGCATTACAAAGAGTGTT
CTATGAATTACAGCATAGTGATAAACCTGTAGGAACAAAAAAGTTAACAA
AGTCATTTGGGTGGGAAACTTTAGATAGCTTCATGCAACATGATGTTCAG
GAGCTTTGTCGAGTGTTGCTCGATAATGTGGAAAATAAGATGAAAGGCAC
CTGTGTAGAGGGCACCATACCCAAATTATTCCGCGGCAAAATGGTGTCCT
ATATCCAGTGTAAAGAAGTAGACTATCGGTCTGATAGAAGAGAAGATTAT
TATGATATCCAGCTAAGTATCAAAGGAAAGAAAAATATATTTGAATCATT
TGTGGATTATGTGGCAGTAGAACAGCTCGATGGGGACAATAAATACGACG
CTGGGGAACATGGCTTACAGGAAGCAGAGAAAGGTGTGAAATTCCTAACA
TTGCCACCAGTGTTACATCTACAACTGATGAGATTTATGTATGACCCTCA
GACGGACCAAAATATCAAGATCAATGATAGGTTTGAATTCCCAGAGCAGT
TACCACTTGATGAATTTTTGCAAAAAACAGATCCTAAGGACCCTGCAAAT
TATATTCTTCATGCAGTCCTGGTTCATAGTGGAGATAATCATGGTGGACA
TTATGTGGTTTATCTAAACCCCAAAGGGGATGGCAAATGGTGTAAATTTG
ATGACGACGTGGTGTCAAGGTGTACTAAAGAGGAAGCAATTGAGCACAAT
TATGGGGGTCACGATGACGACCTGTCTGTTCGACACTGCACTAATGCTTA
CATGTTAGTCTACATCAGGGAATCAAAACTGAGTGAAGTTTTACAGGCGG
TCACCGACCATGATATTCCTCAGCAGTTGGTGGAGCGATTACAAGAAGAG
AAAAGGATCTAAGCGGCCGC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSDGHNTAEEDMEDD
TSWRSEATFQFTVERFSRLSESVLSPPCFVRNLPWKIMVMPRFYPDRPHQ
KSVGFFLQCNAESDSTSWSCHAQAVLKIINYRDDEKSFSRRISHLFFHKE
NDWGFSNFMAWSEVTDPEKGFIDDDKVTFEVFVQADAPHGVAWDSKKHTG
YVGLKNQGATCYMNSLLQTLFFTNQLRKAVYMMPTEGDDSSKSVPLALQR
VFYELQHSDKPVGTKKLTKSFGWETLDSFMQHDVQELCRVLLDNVENKMK
GTCVEGTIPKLFRGKMVSYIQCKEVDYRSDRREDYYDIQLSIKGKKNIFE
SFVDYVAVEQLDGDNKYDAGEHGLQEAEKGVKFLTLPPVLHLQLMRFMYD
PQTDQNIKINDRFEFPEQLPLDEFLQKTDPKDPANYILHAVLVHSGDNHG
GHYVVYLNPKGDGKWCKFDDDVVSRCTKEEAIEHNYGGHDDDLSVRHCTN
AYMLVYIRESKLSEVLQAVTDHDIPQQLVERLQEEKRI*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU32687
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for mammalian expression; ; Estimated MW of expressed protein : 85461 Daltons